##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261778_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22865678
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80666779266287	32.0	32.0	32.0	32.0	32.0
2	31.24410791580289	32.0	32.0	32.0	32.0	32.0
3	34.91983404122108	37.0	32.0	37.0	32.0	37.0
4	35.49843289142793	37.0	37.0	37.0	32.0	37.0
5	35.83344543730564	37.0	37.0	37.0	32.0	37.0
6	39.01590663526356	41.0	41.0	41.0	37.0	41.0
7	39.2191349847575	41.0	41.0	41.0	37.0	41.0
8	39.38640367453788	41.0	41.0	41.0	37.0	41.0
9	39.35515566168648	41.0	41.0	41.0	37.0	41.0
10-14	39.372143445735574	41.0	41.0	41.0	37.0	41.0
15-19	39.294168526295174	41.0	41.0	41.0	37.0	41.0
20-24	39.268281815216675	41.0	41.0	41.0	37.0	41.0
25-29	39.11826158839462	41.0	41.0	41.0	37.0	41.0
30-34	39.109350687086554	41.0	41.0	41.0	37.0	41.0
35-39	39.05353670247609	41.0	41.0	41.0	37.0	41.0
40-44	38.947871993999044	41.0	41.0	41.0	36.0	41.0
45-49	38.86998426200177	41.0	41.0	41.0	32.0	41.0
50-54	38.80181011033218	41.0	41.0	41.0	32.0	41.0
55-59	38.677870474691375	41.0	41.0	41.0	32.0	41.0
60-64	38.61386250606695	41.0	41.0	41.0	32.0	41.0
65-69	38.4832522963019	41.0	41.0	41.0	32.0	41.0
70-74	38.370943227662	41.0	41.0	41.0	32.0	41.0
75-79	37.88228972698732	41.0	38.6	41.0	31.0	41.0
80-84	38.5493686301364	41.0	41.0	41.0	32.0	41.0
85-89	38.47749984059078	41.0	41.0	41.0	32.0	41.0
90-94	38.37706978118034	41.0	41.0	41.0	32.0	41.0
95-99	38.212118363601554	41.0	39.4	41.0	32.0	41.0
100-104	37.97569615036126	41.0	37.0	41.0	32.0	41.0
105-109	37.78778176619123	41.0	37.0	41.0	29.0	41.0
110-114	37.53843673474279	41.0	37.0	41.0	27.0	41.0
115-119	37.15718494767573	41.0	37.0	41.0	27.0	41.0
120-124	36.788667198059905	41.0	37.0	41.0	27.0	41.0
125-129	36.41062654691455	41.0	37.0	41.0	24.0	41.0
130-134	35.95594253535802	41.0	34.0	41.0	22.0	41.0
135-139	35.425126943535204	41.0	32.0	41.0	22.0	41.0
140-144	34.88452892584248	41.0	32.0	41.0	22.0	41.0
145-149	34.15420984236724	37.8	31.0	41.0	16.0	41.0
150	29.280855743704603	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	44.0
13	189.0
14	1335.0
15	6254.0
16	17364.0
17	29116.0
18	37912.0
19	47667.0
20	58648.0
21	72687.0
22	88931.0
23	107993.0
24	127937.0
25	150893.0
26	176025.0
27	206339.0
28	239348.0
29	278280.0
30	326604.0
31	380864.0
32	449456.0
33	533927.0
34	647548.0
35	798278.0
36	1021617.0
37	1387495.0
38	2115494.0
39	4143175.0
40	9414254.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.77797748414625	23.908864481779283	7.664818890852517	31.648339143221953
2	26.72871588879205	26.35009615642028	29.662895239981612	17.25829271480606
3	20.707835560353818	26.93805974176668	28.13208950112916	24.222015196750345
4	23.046852142324404	35.268234775281975	21.369416642707904	20.315496439685717
5	25.732088878672666	36.72392473619102	18.867221403962407	18.676764981173903
6	21.757378023078957	36.55795817644244	19.606433712571306	22.0782300879073
7	20.659340986142976	20.503303020097142	34.60021082334682	24.23714517041306
8	19.715273695361233	23.969838112825695	27.022356389344765	29.292531802468314
9	20.8804086194164	22.6035939104889	29.503301848298573	27.012695621796123
10-14	24.163935865579504	27.37383897529592	23.87587234759828	24.5863528115263
15-19	24.05149191936235	26.885222486169862	24.524900805339154	24.538384789128635
20-24	23.951217188102124	27.095914443614554	24.717425145208296	24.235443223075023
25-29	23.675557063068375	27.003358569964455	24.914865435165066	24.40621893180211
30-34	23.64227037500838	27.14179136684594	24.86129773416535	24.35464052398033
35-39	23.404603849148227	27.295253647955963	24.83968544489412	24.46045705800169
40-44	23.45665117946895	27.00150176713525	25.08622867448946	24.455618378906344
45-49	23.43364740356971	27.024840736642393	25.08177310653661	24.459738753251283
50-54	23.57189059903914	27.111187534263152	24.94049682652657	24.37642504017114
55-59	23.6201725431974	26.988454420742098	24.97203333032761	24.4193397057329
60-64	23.55067541492743	26.81773495375724	25.345231433532994	24.286358197782338
65-69	23.871909070686527	26.697766260636353	25.410449774259536	24.019874894417587
70-74	23.907063609067638	26.846579940827688	25.247348439908592	23.999008010196086
75-79	23.834690797992977	26.918236563319798	25.141592440365486	24.10548019832174
80-84	23.630626342833402	27.150964602632044	25.045266602511028	24.173142452023523
85-89	23.72349751218285	27.029554691002893	24.900711188635878	24.34623660817838
90-94	23.623584364471146	27.256773618627978	24.9934177947266	24.12622422217428
95-99	23.531805444466833	27.271048371722223	25.035369277226106	24.16177690658484
100-104	23.998728082657202	27.494303591842232	24.641852091344337	23.865116234156226
105-109	23.980923113417305	27.793458603448258	24.406059134482334	23.819559148652107
110-114	24.081909900096527	27.738705455221275	24.402380382967422	23.777004261714772
115-119	24.387667092003056	27.786195638715345	24.305490611023696	23.520646658257903
120-124	24.791386798088293	27.972041722159315	24.055980406936293	23.180591072816103
125-129	25.34906841427349	28.07771725117102	23.77788258128681	22.79533175326868
130-134	26.0186860457886	28.106894771093977	23.43405206635887	22.44036711675856
135-139	26.366313258830466	28.172973995923307	23.52628257050008	21.93443017474615
140-144	26.809579659658382	28.35322681472713	23.22234163491083	21.614851890703658
145-149	27.531882634127463	28.01181486013786	23.31584852895382	21.14045397678085
150	27.47081284247614	28.0976890769625	23.40818583495089	21.023312245610473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	107.0
2	96.0
3	102.0
4	121.0
5	148.0
6	181.5
7	217.5
8	260.0
9	280.0
10	303.0
11	365.0
12	421.5
13	481.0
14	591.0
15	739.5
16	896.5
17	1137.5
18	1601.0
19	2318.5
20	3545.5
21	5409.0
22	8256.0
23	11928.0
24	17027.5
25	23913.5
26	34007.0
27	47861.5
28	64316.0
29	88811.5
30	114584.5
31	142843.0
32	183237.5
33	246210.5
34	327661.5
35	374904.0
36	409280.0
37	501302.5
38	556481.0
39	540477.5
40	536834.0
41	579172.0
42	642775.5
43	716799.5
44	776405.5
45	842509.5
46	929585.0
47	983624.5
48	1037883.5
49	1113784.5
50	1198966.5
51	1173165.5
52	1079147.0
53	1023222.0
54	946794.0
55	886566.5
56	824747.0
57	723459.0
58	624307.0
59	525659.0
60	436563.5
61	357796.0
62	285378.0
63	227729.5
64	173805.5
65	128335.0
66	95776.5
67	72350.5
68	56499.5
69	43302.0
70	31831.5
71	23343.0
72	18017.0
73	12799.5
74	8260.5
75	5440.0
76	3520.5
77	2296.5
78	1478.0
79	979.0
80	639.0
81	426.5
82	295.5
83	208.0
84	145.0
85	101.5
86	81.0
87	58.5
88	47.5
89	47.0
90	35.0
91	29.0
92	26.5
93	21.0
94	17.0
95	13.5
96	15.0
97	15.0
98	13.0
99	15.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.461863496896965
2	0.021910568319907245
3	0.0
4	0.0
5	3.4986935440969646E-4
6	0.0
7	3.8922965678078736E-4
8	0.0
9	0.0
10-14	7.233548902420475E-4
15-19	1.3994774176387859E-4
20-24	9.385245432040108E-4
25-29	2.3353779406847243E-4
30-34	0.0022496599488543486
35-39	1.635639231865331E-4
40-44	3.603654350419874E-4
45-49	4.023497575711509E-4
50-54	2.97388951248242E-5
55-59	0.0018551822517574156
60-64	3.901043301668116E-4
65-69	5.580416202834659E-4
70-74	5.265533783865932E-4
75-79	3.7523488260439946E-4
80-84	1.504438223961695E-4
85-89	9.24529769027623E-4
90-94	0.0010539814301592106
95-99	3.096343786525814E-4
100-104	1.801827175209937E-4
105-109	4.4608342687236304E-4
110-114	2.0292422555762395E-4
115-119	5.073105638940599E-5
120-124	1.530678425542422E-4
125-129	1.3732372160580588E-4
130-134	3.524933745677692E-4
135-139	7.810833337196474E-4
140-144	2.7989548352775717E-4
145-149	1.4344643530797555E-4
150	0.0017449734051183613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2865678E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.532065065710402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8401572409121	23.283326425827276
2	12.023570256081186	7.582559992737845
3	4.523386583454127	4.278951602005111
4	2.4225260889311326	3.0554900103822966
5	1.4794234426718096	2.332463812703239
6	0.9841957850123422	1.862023531824426
7	0.6986098575491356	1.5420028038650213
8	0.5197311310441464	1.3110556672607427
9	0.3944351915631846	1.1193620512118656
>10	2.598020305617417	15.722662050347052
>50	0.2691758973559839	5.888753978621005
>100	0.20321121357679833	12.941074862091623
>500	0.0243874870441755	5.336563186546545
>1k	0.01796380295269691	10.72533573556148
>5k	9.904097636455954E-4	2.0580530348359782
>10k+	2.1530647035773704E-4	0.9603212541782125
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.186683465060603E-5	0.0	0.0	0.0	0.0
2	2.186683465060603E-5	0.0	0.0	4.373366930121206E-6	0.0
3	2.186683465060603E-5	0.0	0.0	4.373366930121206E-6	4.373366930121206E-6
4	6.56005039518181E-5	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
5	8.746733860242412E-5	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
6	1.0058743939278774E-4	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
7	1.0496080632290895E-4	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
8	1.2682764097351497E-4	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
9	1.8805477799521187E-4	0.0	0.0	8.746733860242412E-6	1.3120100790363619E-5
10-11	2.5365528194702994E-4	0.0	0.0	1.3120100790363619E-5	1.3120100790363619E-5
12-13	4.0453644103621156E-4	0.0	0.0	1.7493467720484823E-5	1.7493467720484823E-5
14-15	5.313640820097266E-4	0.0	0.0	1.7493467720484823E-5	1.7493467720484823E-5
16-17	6.34138204867575E-4	4.373366930121206E-6	0.0	2.186683465060603E-5	1.7493467720484823E-5
18-19	7.500324285157869E-4	4.373366930121206E-6	0.0	2.186683465060603E-5	1.7493467720484823E-5
20-21	8.221929828627867E-4	4.373366930121206E-6	0.0	2.186683465060603E-5	1.7493467720484823E-5
22-23	8.987269041399079E-4	4.373366930121206E-6	0.0	2.4053518115666633E-5	1.7493467720484823E-5
24-25	0.0010124344443230592	4.373366930121206E-6	0.0	2.6240201580727238E-5	1.7493467720484823E-5
26-27	0.00112395530104115	4.373366930121206E-6	0.0	2.842688504578784E-5	1.7493467720484823E-5
28-29	0.001283583193990574	4.373366930121206E-6	0.0	3.9360302371090855E-5	1.7493467720484823E-5
30-31	0.0014891314397062708	4.373366930121206E-6	0.0	4.373366930121206E-5	1.7493467720484823E-5
32-33	0.0017012397358171492	4.373366930121206E-6	0.0	4.373366930121206E-5	1.7493467720484823E-5
34-35	0.0019986286870653912	4.373366930121206E-6	0.0	4.373366930121206E-5	1.7493467720484823E-5
36-37	0.0023572447753353303	4.373366930121206E-6	0.0	4.592035276627266E-5	1.7493467720484823E-5
38-39	0.0027246075974655115	6.560050395181809E-6	0.0	4.810703623133327E-5	1.7493467720484823E-5
40-41	0.003231918161359571	8.746733860242412E-6	0.0	5.029371969639387E-5	1.7493467720484823E-5
42-43	0.0037676556102994195	8.746733860242412E-6	0.0	5.2480403161454476E-5	2.186683465060603E-5
44-45	0.004528621456140509	8.746733860242412E-6	0.0	5.2480403161454476E-5	2.186683465060603E-5
46-47	0.00536612122325872	8.746733860242412E-6	0.0	7.872060474218171E-5	2.186683465060603E-5
48-49	0.006404795869162506	8.746733860242412E-6	0.0	8.309397167230292E-5	2.4053518115666633E-5
50-51	0.007856753689962746	8.746733860242412E-6	0.0	8.528065513736352E-5	2.6240201580727238E-5
52-53	0.009520819806873866	8.746733860242412E-6	0.0	9.184070553254532E-5	2.6240201580727238E-5
54-55	0.01134888718366453	8.746733860242412E-6	0.0	9.621407246266653E-5	2.6240201580727238E-5
56-57	0.013647091505443224	8.746733860242412E-6	0.0	9.621407246266653E-5	2.6240201580727238E-5
58-59	0.016533513679323222	1.0933417325303016E-5	0.0	9.840075592772713E-5	3.0613568510848444E-5
60-61	0.020145914763603334	1.3120100790363619E-5	0.0	1.0058743939278774E-4	3.0613568510848444E-5
62-63	0.02465922943548842	1.3120100790363619E-5	0.0	1.0714748978796955E-4	3.0613568510848444E-5
64-65	0.030580768258872536	1.3120100790363619E-5	0.0	1.0933417325303015E-4	3.0613568510848444E-5
66-67	0.03781650384475807	1.3120100790363619E-5	0.0	1.0933417325303015E-4	3.0613568510848444E-5
68-69	0.046453903531747456	1.3120100790363619E-5	0.0	1.0933417325303015E-4	3.0613568510848444E-5
70-71	0.05766065629018304	1.3120100790363619E-5	0.0	1.0933417325303015E-4	3.0613568510848444E-5
72-73	0.07151329604134196	1.3120100790363619E-5	0.0	1.1589422364821197E-4	3.280025197590905E-5
74-75	0.08937631326742204	1.3120100790363619E-5	0.0	1.1808090711327257E-4	3.4986935440969646E-5
76-77	0.11128906827079434	1.3120100790363619E-5	0.0	1.1808090711327257E-4	3.4986935440969646E-5
78-79	0.13812404775401804	1.7493467720484823E-5	0.0	1.2245427404339378E-4	3.4986935440969646E-5
80-81	0.171606544971026	1.7493467720484823E-5	0.0	1.2245427404339378E-4	3.4986935440969646E-5
82-83	0.21363897453642094	1.7493467720484823E-5	0.0	1.2682764097351497E-4	3.4986935440969646E-5
84-85	0.26639927318140316	1.7493467720484823E-5	0.0	1.2682764097351497E-4	3.9360302371090855E-5
86-87	0.33256175478374184	1.7493467720484823E-5	0.0	1.2682764097351497E-4	3.9360302371090855E-5
88-89	0.4144705440179819	1.7493467720484823E-5	0.0	1.355743748337574E-4	3.9360302371090855E-5
90-91	0.5117364112273426	2.186683465060603E-5	0.0	1.443211086939998E-4	3.9360302371090855E-5
92-93	0.6312364759094395	2.186683465060603E-5	0.0	1.443211086939998E-4	3.9360302371090855E-5
94-95	0.7781181909410253	2.186683465060603E-5	0.0	1.5088115908918161E-4	3.9360302371090855E-5
96-97	0.9550449367825437	2.186683465060603E-5	0.0	1.5306784255424223E-4	3.9360302371090855E-5
98-99	1.1666568557468535	2.6240201580727235E-5	0.0	1.5306784255424223E-4	4.154698583615145E-5
100-101	1.418608711274601	3.0613568510848444E-5	0.0	1.5306784255424223E-4	4.373366930121206E-5
102-103	1.7100586302317384	3.0613568510848444E-5	0.0	1.5306784255424223E-4	4.373366930121206E-5
104-105	2.0571071629715068	3.0613568510848444E-5	0.0	1.5744120948436342E-4	4.373366930121206E-5
106-107	2.459686522306489	3.0613568510848444E-5	0.0	1.5744120948436342E-4	4.373366930121206E-5
108-109	2.9208318248861893	3.4986935440969646E-5	0.0	1.6618794334460584E-4	4.810703623133327E-5
110-111	3.4362134374497884	3.4986935440969646E-5	0.0	1.6837462680966645E-4	4.810703623133327E-5
112-113	4.011744152086809	3.4986935440969646E-5	0.0	1.7274799373978762E-4	4.810703623133327E-5
114-115	4.660229187168646	3.4986935440969646E-5	0.0	1.7493467720484823E-4	4.810703623133327E-5
116-117	5.384546218135321	3.4986935440969646E-5	0.0	1.7712136066990884E-4	4.810703623133327E-5
118-119	6.181161564507294	3.4986935440969646E-5	0.0	1.7930804413496945E-4	4.810703623133327E-5
120-121	7.036530908901979	3.4986935440969646E-5	0.0	1.7930804413496945E-4	4.810703623133327E-5
122-123	7.959092662811048	3.4986935440969646E-5	0.0	1.7930804413496945E-4	4.810703623133327E-5
124-125	8.944106096482248	3.4986935440969646E-5	0.0	1.7930804413496945E-4	4.810703623133327E-5
126-127	9.98193230920159	3.4986935440969646E-5	0.0	1.8586809453015126E-4	5.2480403161454476E-5
128-129	11.089371590031137	3.4986935440969646E-5	0.0	1.9242814492533307E-4	5.2480403161454476E-5
130-131	12.249918852176613	3.4986935440969646E-5	0.0	1.9680151185545426E-4	5.2480403161454476E-5
132-133	13.439640845112924	3.9360302371090855E-5	0.0	2.0117487878557549E-4	5.2480403161454476E-5
134-135	14.669350718574801	3.9360302371090855E-5	0.0	2.0992161264581788E-4	5.2480403161454476E-5
136-137	15.92857863213153	3.9360302371090855E-5	0.0	2.1648166304099968E-4	5.685377009157568E-5
138-139	17.207044986813862	4.3733669301212065E-5	0.0	2.230417134361815E-4	5.904045355663628E-5
>>END_MODULE
