##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22865678
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.710671645074335	32.0	32.0	32.0	27.0	32.0
2	31.439490488757865	32.0	32.0	32.0	32.0	32.0
3	34.98989100607469	37.0	32.0	37.0	32.0	37.0
4	36.02176506640214	37.0	37.0	37.0	32.0	37.0
5	36.291618862121645	37.0	37.0	37.0	37.0	37.0
6	39.630472929777106	41.0	41.0	41.0	37.0	41.0
7	39.76424237234514	41.0	41.0	41.0	37.0	41.0
8	40.02353483679775	41.0	41.0	41.0	37.0	41.0
9	40.06148162324336	41.0	41.0	41.0	37.0	41.0
10-14	40.059912686603916	41.0	41.0	41.0	37.0	41.0
15-19	40.0085003121272	41.0	41.0	41.0	37.0	41.0
20-24	39.952308809736586	41.0	41.0	41.0	37.0	41.0
25-29	39.86426583983208	41.0	41.0	41.0	37.0	41.0
30-34	39.81936037934234	41.0	41.0	41.0	37.0	41.0
35-39	39.72872597961014	41.0	41.0	41.0	37.0	41.0
40-44	39.606719608314265	41.0	41.0	41.0	37.0	41.0
45-49	39.582236109508756	41.0	41.0	41.0	37.0	41.0
50-54	39.622793682304106	41.0	41.0	41.0	37.0	41.0
55-59	39.570879210316875	41.0	41.0	41.0	37.0	41.0
60-64	39.51831518837972	41.0	41.0	41.0	37.0	41.0
65-69	39.494405728970726	41.0	41.0	41.0	37.0	41.0
70-74	39.44680478750729	41.0	41.0	41.0	37.0	41.0
75-79	39.09020180376895	41.0	40.2	41.0	35.0	41.0
80-84	39.49914007360726	41.0	41.0	41.0	37.0	41.0
85-89	39.48060579703781	41.0	41.0	41.0	37.0	41.0
90-94	39.44029290537547	41.0	41.0	41.0	37.0	41.0
95-99	39.40330003772466	41.0	41.0	41.0	37.0	41.0
100-104	39.315903451452435	41.0	41.0	41.0	37.0	41.0
105-109	39.194603370169034	41.0	41.0	41.0	37.0	41.0
110-114	39.10367840393799	41.0	41.0	41.0	37.0	41.0
115-119	38.91582373372004	41.0	41.0	41.0	35.0	41.0
120-124	38.748159437913884	41.0	41.0	41.0	32.0	41.0
125-129	38.655720385811435	41.0	41.0	41.0	32.0	41.0
130-134	38.403209360334735	41.0	37.0	41.0	32.0	41.0
135-139	38.18373995295482	41.0	37.0	41.0	32.0	41.0
140-144	37.87190072387095	41.0	37.0	41.0	32.0	41.0
145-149	37.56624418484332	41.0	37.0	41.0	29.0	41.0
150	34.42059977403688	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	69.0
17	168.0
18	364.0
19	997.0
20	2427.0
21	4988.0
22	9005.0
23	15797.0
24	26377.0
25	42407.0
26	63462.0
27	90884.0
28	123339.0
29	163024.0
30	207711.0
31	259146.0
32	317490.0
33	386159.0
34	472089.0
35	583019.0
36	741590.0
37	993335.0
38	1485104.0
39	3001783.0
40	1.3874937E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.20958800870727	11.83869629401438	5.928427095753208	33.02328860152514
2	25.894513009626248	12.697808211025693	39.82128824983762	21.58639052951044
3	23.18412775689398	17.078911895811704	26.27277441762278	33.46418592967154
4	25.569663842900265	26.139299258915482	24.909040527903876	23.381996370280383
5	27.33939050484311	29.42595010740552	25.181024590655042	18.05363479709633
6	22.39704365208145	30.07200440724775	26.79254375276306	20.738408187907744
7	18.26003147599647	22.27984667675282	42.94081723708346	16.519304610167257
8	19.201722336857888	21.434295541116253	34.525225974055964	24.838756147969896
9	19.482269452058233	19.833958127110858	36.796778997762495	23.886993423068407
10-14	24.45543456696354	25.6802527261881	27.376027695955436	22.488285010892927
15-19	24.581590396410817	24.92027107994633	27.587114687286764	22.911023836356094
20-24	24.36879876065042	24.990579764831043	27.60377836623488	23.03684310828365
25-29	24.122312245321716	25.09566328901391	27.784263562034077	22.997760903630304
30-34	24.16430511893515	25.215155446679987	27.2937437083584	23.326795726026468
35-39	24.393046981160996	25.091485800991535	27.34891897640164	23.166548241445824
40-44	24.24806219179682	25.08758147410093	27.564503477853762	23.099852856248493
45-49	24.18178180408429	25.052330763347847	27.61497290223811	23.150914530329747
50-54	23.9803802526545	25.37654357864548	27.309385383258487	23.33369078544153
55-59	24.413859103527173	25.168344628731802	27.12672450797955	23.29107175976147
60-64	23.93784130872641	25.121107724658405	27.442539524586685	23.4985114420285
65-69	24.24347653250676	25.348840642920173	27.289317997133995	23.118364827439066
70-74	24.149708581286024	25.214053415934224	27.22844854516573	23.407789457614022
75-79	24.156450629568845	25.217618309633288	27.029071619824542	23.59685944097333
80-84	23.997649748060475	25.12155382704838	27.32743509851041	23.553361326380738
85-89	24.414017261049157	24.958886426000294	27.124838817654982	23.502257495295567
90-94	24.375186695460158	25.241026785648295	27.047796688087374	23.335989830804177
95-99	24.61927650855321	25.29187035622232	26.768434458383616	23.320418676840855
100-104	24.274549950208595	25.211944434135408	26.973300958284383	23.54020465737161
105-109	24.600015915656606	25.28797358371867	26.545578107361827	23.566432393262897
110-114	24.76084246527756	25.451024133782692	26.46846523779486	23.319668163144897
115-119	24.591508115563	25.949360174069785	26.06496876412838	23.394162946238836
120-124	24.8702491147547	25.870794469820265	25.805623712911885	23.453332702513155
125-129	24.690251338583955	25.996291951793378	25.695398550193495	23.61805815942917
130-134	24.741922403887028	26.060259535396256	25.45299894107546	23.744819119641257
135-139	24.644817561220027	26.36673139877232	24.993194307725368	23.995256732282293
140-144	24.697850163623176	26.463646395332024	24.74018463655562	24.09831880448918
145-149	24.482758566398914	26.556203558533664	24.68187342448604	24.279164450581384
150	24.399576212348663	26.12785135505992	24.666238797125327	24.806333635466085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	121.0
2	74.0
3	72.0
4	93.5
5	105.0
6	115.0
7	138.0
8	151.5
9	165.0
10	195.0
11	231.5
12	265.5
13	310.5
14	384.0
15	501.0
16	653.0
17	890.5
18	1301.0
19	1934.0
20	3183.0
21	5249.0
22	7887.5
23	11188.5
24	16513.5
25	24606.5
26	34728.5
27	47583.5
28	66527.0
29	92793.0
30	120985.0
31	151439.0
32	193023.0
33	248456.0
34	317029.5
35	386512.0
36	435698.5
37	522038.0
38	596203.0
39	569292.5
40	546919.0
41	603194.0
42	670072.0
43	737380.5
44	821038.0
45	897312.0
46	957761.0
47	999965.0
48	1040336.5
49	1091961.0
50	1147646.0
51	1139355.5
52	1093167.5
53	1033511.0
54	933632.5
55	866740.0
56	795842.5
57	694722.0
58	611264.5
59	528205.5
60	435886.0
61	345672.0
62	270459.0
63	205561.0
64	155798.5
65	115098.5
66	80454.5
67	58605.0
68	41978.5
69	28232.0
70	19428.0
71	13174.5
72	9485.5
73	6443.0
74	4010.0
75	2545.5
76	1734.5
77	1059.5
78	540.0
79	284.5
80	174.0
81	122.0
82	78.0
83	43.5
84	28.0
85	12.5
86	5.0
87	4.5
88	2.5
89	3.5
90	4.0
91	2.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4192537828967942
2	0.20520274972821712
3	0.0
4	0.0
5	0.0
6	0.0066562644676444755
7	0.0
8	0.0
9	0.0
10-14	6.297648379374537E-5
15-19	4.46083426872363E-5
20-24	2.97388951248242E-5
25-29	5.011878501918902E-4
30-34	1.0496080632290894E-4
35-39	2.3266312068244817E-4
40-44	0.0012376628412243014
45-49	0.002372114222897742
50-54	6.297648379374537E-4
55-59	3.6561347535813283E-4
60-64	5.501695598092477E-4
65-69	4.7407297522513873E-4
70-74	8.475585110574897E-4
75-79	4.058484511152479E-4
80-84	0.09248971318497531
85-89	5.046865437359872E-4
90-94	0.0010137464544020956
95-99	3.5336804795379345E-4
100-104	3.7523488260439946E-4
105-109	6.262661443933566E-4
110-114	0.001722231897081731
115-119	0.08280008141459877
120-124	9.087856480791867E-4
125-129	7.504697652087989E-4
130-134	0.0017659655663829431
135-139	0.0011362007284454894
140-144	6.638770999923991E-4
145-149	0.0012350388210662286
150	0.0019811352193449066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2865678E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.9417117139971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.54376551237873	26.396284763233336
2	11.683382581964429	8.164747720466723
3	4.260055033777873	4.465608446280859
4	2.2103234009587704	3.0892993228401195
5	1.303234436031089	2.2768620979775953
6	0.8493636467977319	1.7806931812053364
7	0.5943139600073959	1.4536442940727698
8	0.4535859661174498	1.267925605247263
9	0.36447963281241014	1.1461988029819279
>10	2.2977571513947366	15.388850201602333
>50	0.23356042752960166	5.664167039865671
>100	0.17167257113375795	11.926936608680725
>500	0.019616571478837736	4.722283179982859
>1k	0.01385207027580345	9.444863253125106
>5k	9.074076736874286E-4	2.1906977885904526
>10k+	1.296296676696312E-4	0.6209376938472118
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT	23346	0.10210062435060968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0613568510848444E-5	4.373366930121206E-6	0.0	4.373366930121206E-6	0.0
2	3.4986935440969646E-5	1.3120100790363619E-5	0.0	4.373366930121206E-6	4.373366930121206E-6
3	6.56005039518181E-5	1.3120100790363619E-5	0.0	4.373366930121206E-6	4.373366930121206E-6
4	1.355743748337574E-4	1.3120100790363619E-5	0.0	1.7493467720484823E-5	8.746733860242412E-6
5	1.7056131027472704E-4	1.3120100790363619E-5	0.0	2.186683465060603E-5	1.3120100790363619E-5
6	1.9242814492533307E-4	1.3120100790363619E-5	0.0	2.186683465060603E-5	1.3120100790363619E-5
7	2.099216126458179E-4	1.3120100790363619E-5	0.0	2.186683465060603E-5	1.3120100790363619E-5
8	2.4053518115666633E-4	1.3120100790363619E-5	0.0	2.6240201580727238E-5	1.7493467720484823E-5
9	3.0613568510848445E-4	1.3120100790363619E-5	0.0	3.9360302371090855E-5	2.186683465060603E-5
10-11	3.8266960638560555E-4	1.7493467720484823E-5	0.0	4.810703623133327E-5	2.186683465060603E-5
12-13	5.18243981219363E-4	1.7493467720484823E-5	0.0	5.904045355663628E-5	2.6240201580727235E-5
14-15	6.406982552627567E-4	1.9680151185545428E-5	0.0	6.122713702169689E-5	3.280025197590905E-5
16-17	7.347256442603626E-4	2.186683465060603E-5	0.0	6.77871874168787E-5	3.4986935440969646E-5
18-19	8.353130836531503E-4	2.4053518115666633E-5	4.373366930121206E-6	7.21605543469999E-5	3.4986935440969646E-5
20-21	8.856068033495442E-4	2.6240201580727238E-5	8.746733860242412E-6	7.43472378120605E-5	3.4986935440969646E-5
22-23	9.271537891856957E-4	3.9360302371090855E-5	8.746733860242412E-6	8.309397167230292E-5	3.717361890603025E-5
24-25	0.0010036877104628167	3.9360302371090855E-5	8.746733860242412E-6	8.528065513736352E-5	3.9360302371090855E-5
26-27	0.0010627281640194532	3.9360302371090855E-5	1.0933417325303016E-5	8.746733860242412E-5	3.9360302371090855E-5
28-29	0.0011480088191568164	4.154698583615145E-5	1.3120100790363619E-5	8.965402206748471E-5	3.9360302371090855E-5
30-31	0.0012595296758749074	4.373366930121206E-5	1.3120100790363619E-5	9.621407246266653E-5	3.9360302371090855E-5
32-33	0.001371050532592998	4.592035276627266E-5	1.3120100790363619E-5	9.621407246266653E-5	5.029371969639387E-5
34-35	0.0015612919940532705	5.2480403161454476E-5	1.5306784255424222E-5	9.621407246266653E-5	5.685377009157568E-5
36-37	0.0018105739090701792	5.2480403161454476E-5	1.7493467720484823E-5	1.0058743939278774E-4	6.122713702169689E-5
38-39	0.002088282709132876	5.2480403161454476E-5	1.7493467720484823E-5	1.0933417325303015E-4	7.872060474218171E-5
40-41	0.0024862590997739055	5.2480403161454476E-5	1.7493467720484823E-5	1.2682764097351497E-4	8.309397167230292E-5
42-43	0.002932342526646269	5.466708662651508E-5	1.7493467720484823E-5	1.2682764097351497E-4	9.402738899760594E-5
44-45	0.0035599206811186617	5.685377009157568E-5	1.7493467720484823E-5	1.2901432443857558E-4	9.621407246266653E-5
46-47	0.004294646325379025	5.685377009157568E-5	1.7493467720484823E-5	1.355743748337574E-4	9.621407246266653E-5
48-49	0.005239293582285205	5.685377009157568E-5	1.7493467720484823E-5	1.5088115908918161E-4	9.840075592772713E-5
50-51	0.0065491169778565065	5.685377009157568E-5	1.7493467720484823E-5	1.5306784255424223E-4	1.1370754018315136E-4
52-53	0.00808198208686399	5.685377009157568E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.246409575084544E-4
54-55	0.009820395441587169	6.56005039518181E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.2682764097351497E-4
56-57	0.012018012323973076	6.56005039518181E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.312010079036362E-4
58-59	0.01479291364113498	6.997387088193929E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.355743748337574E-4
60-61	0.018293793868697006	6.997387088193929E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.355743748337574E-4
62-63	0.022658414064957968	6.997387088193929E-5	1.7493467720484823E-5	1.6618794334460584E-4	1.355743748337574E-4
64-65	0.028446565196973386	7.43472378120605E-5	2.186683465060603E-5	1.7056131027472704E-4	1.443211086939998E-4
66-67	0.035472379170213106	7.872060474218171E-5	2.186683465060603E-5	1.7930804413496945E-4	1.5525452601930284E-4
68-69	0.04400481805087958	7.872060474218171E-5	2.4053518115666633E-5	1.8368141106509065E-4	1.6181457641448462E-4
70-71	0.0551875172911995	7.872060474218171E-5	2.842688504578784E-5	1.8368141106509065E-4	1.6181457641448462E-4
72-73	0.06889364925019936	7.872060474218171E-5	3.0613568510848444E-5	1.9024146146027246E-4	1.6618794334460584E-4
74-75	0.08684850718181197	7.872060474218171E-5	3.0613568510848444E-5	1.9898819532051487E-4	1.6618794334460584E-4
76-77	0.10874595540092885	7.872060474218171E-5	3.0613568510848444E-5	2.0117487878557549E-4	1.7274799373978762E-4
78-79	0.13573837609363693	7.872060474218171E-5	3.4986935440969646E-5	2.0336156225063607E-4	1.7712136066990884E-4
80-81	0.16917932632480875	7.872060474218171E-5	3.9360302371090855E-5	2.077349291807573E-4	1.8149472760003007E-4
82-83	0.21164034584935554	7.872060474218171E-5	3.9360302371090855E-5	2.099216126458179E-4	1.8368141106509065E-4
84-85	0.26491670179209204	7.872060474218171E-5	5.904045355663628E-5	2.142949795759391E-4	1.8368141106509065E-4
86-87	0.3315099600370477	7.872060474218171E-5	6.122713702169689E-5	2.142949795759391E-4	1.8805477799521187E-4
88-89	0.414151288232083	7.872060474218171E-5	6.122713702169689E-5	2.142949795759391E-4	1.9242814492533307E-4
90-91	0.5128078861252223	8.090728820724232E-5	6.122713702169689E-5	2.2741508036630271E-4	1.9242814492533307E-4
92-93	0.6338561227005821	8.309397167230292E-5	6.122713702169689E-5	2.3178844729642394E-4	1.9242814492533307E-4
94-95	0.782511238022332	8.528065513736352E-5	6.122713702169689E-5	2.3616181422654513E-4	2.2741508036630271E-4
96-97	0.9612922914422217	9.402738899760594E-5	6.341382048675749E-5	2.4490854808678755E-4	2.2741508036630271E-4
98-99	1.1747738247691584	9.621407246266653E-5	6.56005039518181E-5	2.492819150169088E-4	2.2741508036630271E-4
100-101	1.4288839368769208	9.621407246266653E-5	6.56005039518181E-5	2.558419654120906E-4	2.2741508036630271E-4
102-103	1.7234520664552346	9.621407246266653E-5	6.56005039518181E-5	2.5802864887715116E-4	2.2960176383136333E-4
104-105	2.073629743233505	9.621407246266653E-5	6.56005039518181E-5	2.5802864887715116E-4	2.3616181422654513E-4
106-107	2.482662005473881	9.621407246266653E-5	6.56005039518181E-5	2.624020158072724E-4	2.4053518115666633E-4
108-109	2.947395655619746	9.621407246266653E-5	6.56005039518181E-5	2.711487496675148E-4	2.4490854808678755E-4
110-111	3.468353311019249	9.621407246266653E-5	6.56005039518181E-5	2.711487496675148E-4	2.4490854808678755E-4
112-113	4.0500854599631815	1.0058743939278774E-4	6.56005039518181E-5	2.820821669928178E-4	2.4490854808678755E-4
114-115	4.707406882927329	1.0058743939278774E-4	6.56005039518181E-5	2.886422173879996E-4	2.4490854808678755E-4
116-117	5.441340947773339	1.0058743939278774E-4	6.56005039518181E-5	2.930155843181208E-4	2.4490854808678755E-4
118-119	6.248323360453165	1.0058743939278774E-4	6.56005039518181E-5	2.930155843181208E-4	2.492819150169088E-4
120-121	7.118277883559806	1.0058743939278774E-4	6.56005039518181E-5	2.930155843181208E-4	2.492819150169088E-4
122-123	8.056102688054997	1.0496080632290895E-4	6.56005039518181E-5	2.97388951248242E-4	2.492819150169088E-4
124-125	9.05861396281361	1.0496080632290895E-4	6.56005039518181E-5	3.0176231817836323E-4	2.5365528194702994E-4
126-127	10.11880776069706	1.0496080632290895E-4	6.56005039518181E-5	3.0613568510848445E-4	2.5802864887715116E-4
128-129	11.248354411358369	1.0496080632290895E-4	6.56005039518181E-5	3.126957355036662E-4	2.624020158072724E-4
130-131	12.43294644488565	1.0496080632290895E-4	6.56005039518181E-5	3.1488241896872684E-4	2.624020158072724E-4
132-133	13.651961249519914	1.0496080632290895E-4	6.56005039518181E-5	3.1488241896872684E-4	2.624020158072724E-4
134-135	14.916465630277834	1.0933417325303015E-4	6.56005039518181E-5	3.1488241896872684E-4	2.711487496675148E-4
136-137	16.21154421924423	1.0933417325303015E-4	6.56005039518181E-5	3.1925578589884807E-4	2.842688504578784E-4
138-139	17.53159910674855	1.1152085671809075E-4	6.77871874168787E-5	3.2144246936390865E-4	2.995756347133026E-4
>>END_MODULE
