##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261777_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23460373
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.645279808637312	32.0	32.0	32.0	32.0	32.0
2	31.323392471210923	32.0	32.0	32.0	32.0	32.0
3	34.8597345830776	37.0	32.0	37.0	32.0	37.0
4	35.5895264751332	37.0	37.0	37.0	32.0	37.0
5	35.903798076867744	37.0	37.0	37.0	37.0	37.0
6	39.132303352551126	41.0	41.0	41.0	37.0	41.0
7	39.33076298488519	41.0	41.0	41.0	37.0	41.0
8	39.42559186931938	41.0	41.0	41.0	37.0	41.0
9	39.39881139144719	41.0	41.0	41.0	37.0	41.0
10-14	39.4274120705583	41.0	41.0	41.0	37.0	41.0
15-19	39.35604219080405	41.0	41.0	41.0	37.0	41.0
20-24	39.3169072546289	41.0	41.0	41.0	37.0	41.0
25-29	39.24495294256404	41.0	41.0	41.0	37.0	41.0
30-34	39.22764732683491	41.0	41.0	41.0	37.0	41.0
35-39	39.13072898713077	41.0	41.0	41.0	37.0	41.0
40-44	39.00677145244025	41.0	41.0	41.0	37.0	41.0
45-49	38.94168553074583	41.0	41.0	41.0	37.0	41.0
50-54	38.88685900262541	41.0	41.0	41.0	32.0	41.0
55-59	38.77877378164448	41.0	41.0	41.0	32.0	41.0
60-64	38.73631568432437	41.0	41.0	41.0	32.0	41.0
65-69	38.625369630738604	41.0	41.0	41.0	32.0	41.0
70-74	38.53223052335954	41.0	41.0	41.0	32.0	41.0
75-79	37.971740202084604	41.0	39.4	41.0	31.0	41.0
80-84	38.63821570100356	41.0	41.0	41.0	32.0	41.0
85-89	38.58520124125904	41.0	41.0	41.0	32.0	41.0
90-94	38.505057178758406	41.0	41.0	41.0	32.0	41.0
95-99	38.363306013932515	41.0	41.0	41.0	32.0	41.0
100-104	38.15243822423454	41.0	37.0	41.0	32.0	41.0
105-109	37.99289180952068	41.0	37.0	41.0	32.0	41.0
110-114	37.77055105645592	41.0	37.0	41.0	28.0	41.0
115-119	37.41919724805739	41.0	37.0	41.0	27.0	41.0
120-124	37.08810830075038	41.0	37.0	41.0	27.0	41.0
125-129	36.75065874698582	41.0	37.0	41.0	27.0	41.0
130-134	36.344111775204944	41.0	37.0	41.0	22.0	41.0
135-139	35.85162429429404	41.0	32.0	41.0	22.0	41.0
140-144	35.35808949840653	41.0	32.0	41.0	22.0	41.0
145-149	34.68120407122257	41.0	32.0	41.0	22.0	41.0
150	29.744410372332954	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	34.0
13	183.0
14	1374.0
15	6754.0
16	20854.0
17	38566.0
18	50082.0
19	59752.0
20	70865.0
21	83695.0
22	98153.0
23	114104.0
24	132291.0
25	151471.0
26	173968.0
27	197955.0
28	226910.0
29	261093.0
30	300746.0
31	350093.0
32	407228.0
33	482013.0
34	579768.0
35	712220.0
36	905913.0
37	1230043.0
38	1909797.0
39	4002688.0
40	1.0891759E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.594249962216956	24.529444141073437	7.181679489805508	29.6946264069041
2	27.21720191965656	25.652768546345662	29.633874189492815	17.496155344504967
3	20.79795576992744	26.44541926081056	28.725907299086845	24.03071767017515
4	23.05612958498145	35.17613722509868	21.39274597211221	20.374987217807664
5	25.807278954584195	36.58497089465042	18.780213818310532	18.827536332454855
6	22.090083563462525	36.444978943855666	19.39822951664068	22.06670797604113
7	20.851355096033966	20.59594907872634	34.49657683303444	24.056118992205256
8	19.740606852243996	23.820776421585453	26.989340706560803	29.449276019609748
9	21.03848050497748	22.354226848822904	29.319491211840493	27.28780143435912
10-14	24.318765214330433	27.17209370883054	23.859027465280455	24.65011361155857
15-19	24.192006581590967	26.720187560065717	24.424248682643032	24.663557175700284
20-24	24.043049943577483	26.95685041506321	24.64504811770584	24.35505152365347
25-29	23.810474229255117	26.738442184531273	24.909002699768084	24.54208088644552
30-34	23.81270172692459	26.88156396025809	24.84359761437779	24.462136698439533
35-39	23.619634365208096	27.037273767651904	24.796038227530975	24.54705363960902
40-44	23.65451070087679	26.80255795036609	25.01791026972769	24.52502107902943
45-49	23.605870277609462	26.79578104497655	25.010379887382218	24.58796879003177
50-54	23.713548088534232	26.887375567914155	24.9026948332839	24.49638151026771
55-59	23.785053662253397	26.784556200983626	24.8704275029654	24.559962633797575
60-64	23.672454729363896	26.709364776627027	25.19024437285543	24.427936121153643
65-69	23.934826345598744	26.67082303214591	25.21517980693838	24.179170815316965
70-74	23.951900120006396	26.764917055572017	25.109660170796648	24.17352265362494
75-79	23.880784031669954	26.816505145768467	25.04585348574001	24.256857336821568
80-84	23.718119174743418	27.001556669681687	24.992884161099113	24.287439994475786
85-89	23.825475557607792	26.87676576521793	24.84274251116828	24.455016166006
90-94	23.717232767601963	27.077838565660617	24.936764393725603	24.26816427301182
95-99	23.65970243217467	27.13190449031328	24.90997516946199	24.298417908050062
100-104	23.978712688471617	27.273403927758405	24.63183498907355	24.116048394696428
105-109	24.020723994026085	27.531239267225	24.44587348956419	24.002163249184726
110-114	24.061117377770003	27.505356194568726	24.425685406888523	24.007841020772748
115-119	24.34758840943217	27.59602977598109	24.29553805460128	23.760843759985462
120-124	24.632132390507095	27.708287086822814	24.125784836619893	23.533795686050194
125-129	25.117814359438583	27.888175672948247	23.834342797222646	23.159667170390527
130-134	25.74026839329051	27.8688747918376	23.567185985659066	22.823670829212826
135-139	26.136958183091615	27.959296876073626	23.5700698200523	22.33367512078246
140-144	26.520829011847795	28.116207805180025	23.308799742039067	22.05416344093311
145-149	27.207020312489487	27.862098778420663	23.34065431445296	21.590226594636892
150	27.055878486403152	27.88703935927595	23.59291847014737	21.46416368417353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	181.5
2	176.0
3	210.0
4	246.5
5	274.0
6	300.5
7	346.5
8	400.5
9	455.0
10	535.0
11	592.0
12	685.5
13	796.5
14	920.0
15	1105.0
16	1324.5
17	1670.0
18	2168.0
19	2981.0
20	4403.5
21	6444.5
22	9478.0
23	13476.0
24	19112.0
25	26440.5
26	36627.0
27	50577.0
28	68138.5
29	93865.5
30	119899.5
31	148295.0
32	187445.0
33	244438.5
34	308585.0
35	349788.0
36	395534.5
37	472800.5
38	513226.0
39	512969.0
40	527361.5
41	583797.0
42	656979.0
43	734955.0
44	796247.5
45	858848.5
46	944563.5
47	1001975.0
48	1054082.0
49	1120138.5
50	1202547.5
51	1201364.5
52	1135150.5
53	1084869.0
54	1005387.5
55	938373.5
56	871935.5
57	770126.5
58	668491.5
59	564139.5
60	467074.0
61	382073.0
62	304979.5
63	243480.0
64	186271.0
65	137150.5
66	102304.0
67	77883.0
68	61467.0
69	48229.5
70	35694.5
71	26791.5
72	21665.5
73	15825.0
74	10513.5
75	7040.0
76	4583.5
77	2981.0
78	1962.5
79	1324.0
80	829.5
81	530.5
82	356.0
83	252.0
84	164.0
85	106.5
86	91.5
87	70.0
88	47.0
89	45.0
90	48.0
91	39.5
92	26.5
93	20.0
94	18.5
95	14.0
96	9.5
97	12.0
98	12.0
99	10.0
100	21.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.568615298657016
2	0.027220368576407545
3	0.0
4	0.0
5	3.4526305272298955E-4
6	0.0
7	3.410005458992489E-4
8	0.0
9	0.0
10-14	7.331511736833852E-4
15-19	1.1594018560574462E-4
20-24	0.00100339410630854
25-29	2.625704203424217E-4
30-34	0.0024066113526839492
35-39	1.9351780979782378E-4
40-44	3.827731127719069E-4
45-49	4.2284067691506866E-4
50-54	2.216503548345118E-5
55-59	0.0019445556129904667
60-64	4.2454567964456493E-4
65-69	5.652084048280051E-4
70-74	5.251408406848434E-4
75-79	4.535307260460011E-4
80-84	1.4066272518344018E-4
85-89	9.309314903049495E-4
90-94	0.001072446716853138
95-99	2.8899796264961344E-4
100-104	2.1397784255177869E-4
105-109	4.586457342344898E-4
110-114	2.5660291078918483E-4
115-119	6.052759689711668E-5
120-124	1.8925530297408315E-4
125-129	1.3810522108919583E-4
130-134	3.606080772884557E-4
135-139	7.612837187200732E-4
140-144	3.248030199690346E-4
145-149	1.636802620316395E-4
150	0.0017646778250286132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3460373E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.539488265448302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15616239745368	23.073079254797207
2	12.214579552791099	7.704831769452792
3	4.670516423124255	4.419170937621329
4	2.4036134686972495	3.032349551626015
5	1.4811670601848943	2.3357625556935013
6	0.9901861318338959	1.873797833135088
7	0.7552362607639931	1.6673835628804912
8	0.5460072573384713	1.3776631588541062
9	0.4496269754770307	1.2762904245198137
>10	2.789393136641838	16.919558865745625
>50	0.2883871181554037	6.309217163510858
>100	0.21434790642978246	13.564480954849357
>500	0.023752061638616987	5.189018068308658
>1k	0.016223407504893705	9.458453152317013
>5k	7.586923876986535E-4	1.6206822752368133
>10k+	4.2149577094368406E-5	0.17826047145156815
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.967509553236856E-5	0.0	0.0	0.0	0.0
2	6.393760235610917E-5	0.0	0.0	0.0	0.0
3	6.393760235610917E-5	0.0	0.0	0.0	0.0
4	1.3640021835969958E-4	0.0	0.0	0.0	0.0
5	1.6197525930214323E-4	0.0	0.0	0.0	0.0
6	1.875503002445869E-4	0.0	0.0	4.262506823740612E-6	0.0
7	1.9607531389206814E-4	0.0	0.0	1.2787520471221834E-5	0.0
8	2.0886283436328997E-4	0.0	0.0	1.2787520471221834E-5	0.0
9	2.6853792989565853E-4	0.0	0.0	1.2787520471221834E-5	0.0
10-11	3.4313179931111924E-4	0.0	0.0	1.2787520471221834E-5	0.0
12-13	4.518257233165048E-4	0.0	0.0	1.491877388309214E-5	0.0
14-15	5.562571404981498E-4	0.0	0.0	1.9181280706832753E-5	0.0
16-17	6.628198110916651E-4	0.0	0.0	2.5575040942443668E-5	0.0
18-19	7.565949612139586E-4	0.0	0.0	2.5575040942443668E-5	0.0
20-21	8.333200840412896E-4	0.0	0.0	2.983754776618428E-5	0.0
22-23	9.82507822872211E-4	4.262506823740612E-6	0.0	2.983754776618428E-5	0.0
24-25	0.0011402205753506135	4.262506823740612E-6	0.0	3.1968801178054586E-5	0.0
26-27	0.0013362958892426816	4.262506823740612E-6	0.0	4.2625068237406115E-5	0.0
28-29	0.0015622087509009341	6.393760235610918E-6	0.0	5.1150081884887336E-5	0.0
30-31	0.0017732028386760945	8.525013647481224E-6	0.0	6.180634894423887E-5	0.0
32-33	0.0020737095697498076	8.525013647481224E-6	0.0	7.033136259172009E-5	0.0
34-35	0.002406185102001575	8.525013647481224E-6	0.0	7.24626160035904E-5	0.0
36-37	0.0027876794627263598	8.525013647481224E-6	0.0	7.672512282733101E-5	4.262506823740612E-6
38-39	0.003233111425807254	8.525013647481224E-6	0.0	8.098762965107161E-5	4.262506823740612E-6
40-41	0.0037659247787748306	8.525013647481224E-6	0.0	8.311888306294192E-5	4.262506823740612E-6
42-43	0.004360544480686646	8.525013647481224E-6	0.0	8.525013647481223E-5	4.262506823740612E-6
44-45	0.005100089414605642	8.525013647481224E-6	0.0	8.525013647481223E-5	4.262506823740612E-6
46-47	0.005816190560994064	8.525013647481224E-6	0.0	8.951264329855283E-5	4.262506823740612E-6
48-49	0.00668787320644902	8.525013647481224E-6	0.0	8.951264329855283E-5	4.262506823740612E-6
50-51	0.00787071885003704	8.525013647481224E-6	0.0	8.951264329855283E-5	4.262506823740612E-6
52-53	0.009168652177866055	8.525013647481224E-6	0.0	8.951264329855283E-5	4.262506823740612E-6
54-55	0.010747910956061951	1.2787520471221834E-5	0.0	9.164389671042315E-5	4.262506823740612E-6
56-57	0.012732107882513208	1.2787520471221834E-5	0.0	9.377515012229345E-5	4.262506823740612E-6
58-59	0.015035992820745008	1.491877388309214E-5	0.0	9.377515012229345E-5	4.262506823740612E-6
60-61	0.017817278523235756	1.7050027294962447E-5	0.0	9.377515012229345E-5	4.262506823740612E-6
62-63	0.021150558859400915	1.7050027294962447E-5	0.0	9.377515012229345E-5	4.262506823740612E-6
64-65	0.025334209306902326	1.7050027294962447E-5	0.0	9.803765694603407E-5	4.262506823740612E-6
66-67	0.030525942618218387	1.7050027294962447E-5	0.0	9.803765694603407E-5	4.262506823740612E-6
68-69	0.036917571600417434	1.7050027294962447E-5	0.0	1.0016891035790437E-4	4.262506823740612E-6
70-71	0.044737140368569586	1.7050027294962447E-5	0.0	1.1082517741725589E-4	4.262506823740612E-6
72-73	0.05489256287613159	1.7050027294962447E-5	0.0	1.1082517741725589E-4	4.262506823740612E-6
74-75	0.06765877081323472	1.7050027294962447E-5	0.0	1.129564308291262E-4	4.262506823740612E-6
76-77	0.08336823971213075	1.7050027294962447E-5	0.0	1.1508768424099651E-4	4.262506823740612E-6
78-79	0.10241738270742753	1.7050027294962447E-5	0.0	1.1721893765286682E-4	4.262506823740612E-6
80-81	0.1266284214662742	2.1312534118703058E-5	0.0	1.1935019106473713E-4	4.262506823740612E-6
82-83	0.15707763896166527	2.1312534118703058E-5	0.0	1.2787520471221835E-4	4.262506823740612E-6
84-85	0.19629696424690263	2.1312534118703058E-5	0.0	1.2787520471221835E-4	4.262506823740612E-6
86-87	0.24550760552698797	2.1312534118703058E-5	0.0	1.3213771153595895E-4	4.262506823740612E-6
88-89	0.30577092700103276	2.1312534118703058E-5	0.0	1.3426896494782926E-4	8.525013647481224E-6
90-91	0.3779181174996663	2.1312534118703058E-5	0.0	1.3640021835969958E-4	8.525013647481224E-6
92-93	0.4690398571241813	2.5575040942443668E-5	0.0	1.4066272518344018E-4	1.2787520471221834E-5
94-95	0.5814570808401043	2.5575040942443668E-5	0.0	1.470564854190511E-4	1.2787520471221834E-5
96-97	0.7175802362562607	2.5575040942443668E-5	0.0	1.6410651271401354E-4	1.2787520471221834E-5
98-99	0.8810431104398895	2.5575040942443668E-5	0.0	1.7050027294962446E-4	1.2787520471221834E-5
100-101	1.077308105885614	2.5575040942443668E-5	0.0	1.7689403318523535E-4	1.2787520471221834E-5
102-103	1.3114156369125078	2.5575040942443668E-5	0.0	1.832877934208463E-4	1.2787520471221834E-5
104-105	1.5901004643020809	2.5575040942443668E-5	0.0	1.8541904683271661E-4	1.2787520471221834E-5
106-107	1.9141468893099014	2.7706294354313976E-5	0.0	1.875503002445869E-4	1.2787520471221834E-5
108-109	2.2911954554175247	2.983754776618428E-5	0.0	1.896815536564572E-4	1.7050027294962447E-5
110-111	2.7200995482893644	2.983754776618428E-5	0.0	1.9394406048019782E-4	1.7050027294962447E-5
112-113	3.203687341202972	2.983754776618428E-5	0.0	2.0033782071580874E-4	1.7050027294962447E-5
114-115	3.754673892013567	2.983754776618428E-5	0.0	2.0033782071580874E-4	1.7050027294962447E-5
116-117	4.373877602031307	3.4100054589924895E-5	0.0	2.0033782071580874E-4	1.7050027294962447E-5
118-119	5.057225645986106	3.62313080017952E-5	0.0	2.0033782071580874E-4	1.7050027294962447E-5
120-121	5.798965770919328	3.8362561413665505E-5	0.0	2.0460032753954934E-4	1.7050027294962447E-5
122-123	6.60965620623338	4.2625068237406115E-5	0.0	2.1312534118703058E-4	1.7050027294962447E-5
124-125	7.4867010852726	4.2625068237406115E-5	0.0	2.1738784801077118E-4	1.7050027294962447E-5
126-127	8.4198320290986	4.2625068237406115E-5	0.0	2.1738784801077118E-4	2.1312534118703058E-5
128-129	9.41808768343112	4.475632164927642E-5	0.0	2.237816082463821E-4	2.1312534118703058E-5
130-131	10.46137672235646	4.9018828473017034E-5	0.0	2.280441150701227E-4	2.1312534118703058E-5
132-133	11.54564123937842	5.1150081884887336E-5	0.0	2.3230662189386333E-4	2.5575040942443668E-5
134-135	12.690968297903874	5.1150081884887336E-5	0.0	2.3870038212947425E-4	2.7706294354313976E-5
136-137	13.865649109671018	5.1150081884887336E-5	0.0	2.472253957769555E-4	2.983754776618428E-5
138-139	15.065900699873783	5.7543842120498254E-5	0.0	2.536191560125664E-4	3.4100054589924895E-5
>>END_MODULE
