##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23460373
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.767230597740284	32.0	32.0	32.0	32.0	32.0
2	31.461802887788696	32.0	32.0	32.0	32.0	32.0
3	35.38218024069779	37.0	32.0	37.0	32.0	37.0
4	36.17596898395435	37.0	37.0	37.0	32.0	37.0
5	36.3924806736875	37.0	37.0	37.0	37.0	37.0
6	39.814380615346565	41.0	41.0	41.0	37.0	41.0
7	39.919145616312235	41.0	41.0	41.0	37.0	41.0
8	40.1346470493031	41.0	41.0	41.0	37.0	41.0
9	40.17068556412125	41.0	41.0	41.0	37.0	41.0
10-14	40.172518211880096	41.0	41.0	41.0	37.0	41.0
15-19	40.13902987816947	41.0	41.0	41.0	37.0	41.0
20-24	40.11267756058269	41.0	41.0	41.0	37.0	41.0
25-29	40.05272733728488	41.0	41.0	41.0	37.0	41.0
30-34	40.00034175927211	41.0	41.0	41.0	37.0	41.0
35-39	39.98710208060204	41.0	41.0	41.0	37.0	41.0
40-44	39.92162053859928	41.0	41.0	41.0	37.0	41.0
45-49	39.87671760376529	41.0	41.0	41.0	37.0	41.0
50-54	39.885327091773	41.0	41.0	41.0	37.0	41.0
55-59	39.82027654036021	41.0	41.0	41.0	37.0	41.0
60-64	39.76187975357425	41.0	41.0	41.0	37.0	41.0
65-69	39.73789835310803	41.0	41.0	41.0	37.0	41.0
70-74	39.69536347098999	41.0	41.0	41.0	37.0	41.0
75-79	39.38219214161684	41.0	40.2	41.0	36.0	41.0
80-84	39.700315429767464	41.0	41.0	41.0	37.0	41.0
85-89	39.68603248550225	41.0	41.0	41.0	37.0	41.0
90-94	39.653218181995655	41.0	41.0	41.0	37.0	41.0
95-99	39.62668504034441	41.0	41.0	41.0	37.0	41.0
100-104	39.555482881708656	41.0	41.0	41.0	37.0	41.0
105-109	39.453194192607256	41.0	41.0	41.0	37.0	41.0
110-114	39.37945302915686	41.0	41.0	41.0	37.0	41.0
115-119	39.212167283103305	41.0	41.0	41.0	37.0	41.0
120-124	39.08010798464287	41.0	41.0	41.0	37.0	41.0
125-129	39.00863053626641	41.0	41.0	41.0	37.0	41.0
130-134	38.793829561021894	41.0	41.0	41.0	33.0	41.0
135-139	38.61338372582567	41.0	41.0	41.0	32.0	41.0
140-144	38.35648342846041	41.0	39.4	41.0	32.0	41.0
145-149	38.11454827252747	41.0	37.0	41.0	32.0	41.0
150	35.21356672376863	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	20.0
16	61.0
17	164.0
18	381.0
19	884.0
20	1932.0
21	4220.0
22	7763.0
23	14046.0
24	23511.0
25	37830.0
26	57620.0
27	82583.0
28	111753.0
29	146163.0
30	184214.0
31	227573.0
32	277290.0
33	333214.0
34	401498.0
35	493006.0
36	622539.0
37	830710.0
38	1227518.0
39	2454103.0
40	1.5919777E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.80750014586772	11.78411629526812	5.406433346416701	31.00195021244746
2	26.345362027828788	12.143370586012066	39.408662990607326	22.102604395551822
3	23.084701168220985	16.787699837508978	26.78324423912612	33.34435475514392
4	25.510229526188695	25.82350672770633	25.07573515561752	23.59052859048746
5	27.284327491297773	29.26621413905056	25.097465415404947	18.35199295424672
6	22.675720702743842	29.81909547567323	26.663897812662174	20.84128600892075
7	18.24479943264329	22.22491517931109	43.01096576767982	16.519319620365795
8	19.129082048269225	21.16126627654215	34.658813821928575	25.05083785326005
9	19.558798148690986	19.659516922429155	36.58143457480407	24.200250354075788
10-14	24.60149604714029	25.509784015648187	27.152191247558594	22.736528689652932
15-19	24.756512468432177	24.714182341128158	27.40887763211334	23.120427558326323
20-24	24.495043308868077	24.862062268382793	27.455912414401812	23.18698200834732
25-29	24.24836830480979	25.008345398643815	27.549985926397476	23.19330037014892
30-34	24.29814510850393	25.127551390115784	27.07407238801402	23.50023111336626
35-39	24.539778839046424	25.0098971421841	27.116692195459535	23.33363182330994
40-44	24.40936879005358	24.90826978536026	27.372703208835226	23.30965821575094
45-49	24.34782291407392	24.854007331526454	27.451278174776807	23.346891579622824
50-54	24.127131765023254	25.234711051747045	27.16753197017745	23.470625213052248
55-59	24.515960010762704	25.006011439151067	27.046603261094276	23.431425288991957
60-64	24.085936531539037	24.91021931954669	27.33296330562962	23.67088084328465
65-69	24.35809617848344	25.257772303433022	27.052305331729155	23.33182618635438
70-74	24.346268549834473	25.082672442142762	27.035999610402587	23.535059397620174
75-79	24.251369321631994	25.0555383723651	26.95319970410225	23.739892601900657
80-84	24.168705316335117	24.986134838578565	27.16035222480367	23.68480762028265
85-89	24.56101349395307	24.889521389018846	26.930899796528564	23.618565320499524
90-94	24.371967749842955	25.097972071094876	26.95377348931161	23.57628668975056
95-99	24.610836327730688	25.12407877396879	26.768454579970168	23.49663031833035
100-104	24.372583992910766	25.090176583727303	26.86787988658841	23.66935953677352
105-109	24.61831304873861	25.18276765963483	26.499337089146163	23.69958220248039
110-114	24.767041409057807	25.26220421446954	26.47283236306292	23.49792201340973
115-119	24.601975561475278	25.69729524783389	26.175643064611926	23.525086126078907
120-124	24.830628065218402	25.67165976248044	25.917373822206308	23.580338350094845
125-129	24.676598966076956	25.8668610955765	25.758065588640488	23.698474349706057
130-134	24.68742499155046	25.922729196516414	25.568597455645193	23.821248356287935
135-139	24.59574473091361	26.31928150704826	25.120493738115897	23.964480023922235
140-144	24.563908105889787	26.5042129766335	24.911129117430605	24.020749800046108
145-149	24.311769985255246	26.773127453984923	24.758686332112383	24.156416228647448
150	24.128415166845496	26.488624763575373	24.728583760857035	24.6543763087221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	114.0
2	77.5
3	72.0
4	74.0
5	83.5
6	102.0
7	122.0
8	126.5
9	143.5
10	170.0
11	191.5
12	231.5
13	284.0
14	356.5
15	439.0
16	599.5
17	959.0
18	1432.5
19	2103.0
20	3356.5
21	5551.5
22	8445.5
23	12051.0
24	17956.0
25	26478.5
26	37222.5
27	50511.5
28	69223.0
29	95746.0
30	123494.5
31	150413.5
32	187860.0
33	239928.5
34	300710.0
35	361355.0
36	412914.0
37	486232.5
38	542769.0
39	535913.5
40	539018.5
41	603810.5
42	675925.5
43	744565.5
44	825612.0
45	906135.5
46	975258.5
47	1022739.0
48	1063086.5
49	1123697.5
50	1198873.5
51	1201161.0
52	1157477.5
53	1097218.5
54	993846.0
55	919245.0
56	841466.0
57	737738.5
58	653705.0
59	570052.5
60	470426.0
61	369858.5
62	288380.5
63	220129.5
64	167754.0
65	123978.0
66	86436.5
67	63013.5
68	45442.0
69	30925.0
70	21495.5
71	14744.0
72	10762.0
73	7485.5
74	4918.0
75	3142.5
76	2063.5
77	1242.0
78	668.5
79	392.0
80	244.0
81	140.0
82	88.0
83	55.0
84	29.5
85	15.0
86	8.0
87	4.5
88	3.0
89	3.0
90	1.5
91	0.0
92	0.5
93	0.5
94	0.5
95	0.5
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5227763002745098
2	0.21226005230181125
3	0.0
4	0.0
5	0.0
6	0.007719399857794248
7	0.0
8	0.0
9	0.0
10-14	6.820010917984978E-5
15-19	5.797009280287231E-5
20-24	1.9607531389206814E-5
25-29	5.950459525941894E-4
30-34	1.065626705935153E-4
35-39	2.7876794627263596E-4
40-44	0.0013963972354574244
45-49	0.0029914272889011613
50-54	7.050186286466972E-4
55-59	4.288081864683055E-4
60-64	6.291460071841142E-4
65-69	6.513110426675655E-4
70-74	0.0010997267605250777
75-79	4.5523572877549727E-4
80-84	0.091261976099016
85-89	7.493486996135994E-4
90-94	0.0013631496822322476
95-99	4.918932874596665E-4
100-104	5.336658543323246E-4
105-109	8.738138988668252E-4
110-114	0.0019292105884250008
115-119	0.08338997849693183
120-124	0.001101431763254574
125-129	9.079139534567502E-4
130-134	0.0021994535210501554
135-139	0.0015225674374401464
140-144	9.138814630099871E-4
145-149	0.0015660450070423006
150	0.001982065673039384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3460373E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.50660706821909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19069709216491	25.600692326765994
2	12.305671657055035	8.492539531610372
3	4.435717638287054	4.591846968298205
4	2.2575813495179164	3.116058902094182
5	1.3880630837029144	2.3948673707618484
6	0.9323207389341747	1.9302735239971942
7	0.6506008261534244	1.571501894643446
8	0.49354982839203054	1.3624583997528612
9	0.3877887802821261	1.2043147559993348
>10	2.4895799584597125	16.48356336002222
>50	0.25581144387200994	6.137826160644041
>100	0.1798174513934235	12.224620033895658
>500	0.019120508586056396	4.505692722512829
>1k	0.01292020146463291	8.373842396853167
>5k	6.564665840128253E-4	1.5645459072014387
>10k+	1.0297515043338185E-4	0.4453557449474538
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0230016376977467E-4	0.0	0.0	0.0	4.262506823740612E-6
2	1.1508768424099651E-4	0.0	0.0	2.983754776618428E-5	1.2787520471221834E-5
3	1.4066272518344018E-4	0.0	0.0	2.983754776618428E-5	1.7050027294962447E-5
4	2.3870038212947425E-4	0.0	0.0	3.4100054589924895E-5	2.5575040942443668E-5
5	2.7280043671939916E-4	0.0	0.0	3.4100054589924895E-5	3.4100054589924895E-5
6	3.282130254280271E-4	0.0	0.0	3.4100054589924895E-5	3.8362561413665505E-5
7	3.367380390755083E-4	0.0	0.0	3.4100054589924895E-5	3.8362561413665505E-5
8	3.5378806637047076E-4	0.0	0.0	4.2625068237406115E-5	3.8362561413665505E-5
9	4.1772566872657995E-4	0.0	0.0	5.5412588708627946E-5	4.6887575061146725E-5
10-11	4.987132983776515E-4	0.0	2.131253411870306E-6	6.393760235610917E-5	5.1150081884887336E-5
12-13	6.13800982618648E-4	0.0	4.262506823740612E-6	7.24626160035904E-5	7.033136259172009E-5
14-15	7.224949066240336E-4	0.0	4.262506823740612E-6	7.672512282733101E-5	7.672512282733101E-5
16-17	8.311888306294193E-4	0.0	4.262506823740612E-6	7.885637623920131E-5	7.885637623920131E-5
18-19	9.164389671042314E-4	2.131253411870306E-6	4.262506823740612E-6	8.098762965107161E-5	8.098762965107161E-5
20-21	9.654577955772485E-4	4.262506823740612E-6	4.262506823740612E-6	8.525013647481223E-5	8.525013647481223E-5
22-23	0.0010421829184045794	4.262506823740612E-6	4.262506823740612E-6	9.803765694603407E-5	8.525013647481223E-5
24-25	0.001101858013936948	4.262506823740612E-6	4.262506823740612E-6	1.0656267059351527E-4	8.525013647481223E-5
26-27	0.0011530080958218353	4.262506823740612E-6	4.262506823740612E-6	1.1082517741725589E-4	8.525013647481223E-5
28-29	0.0012254707118254256	4.262506823740612E-6	4.262506823740612E-6	1.1082517741725589E-4	9.377515012229345E-5
30-31	0.001332033382418941	4.262506823740612E-6	4.262506823740612E-6	1.1508768424099651E-4	9.377515012229345E-5
32-33	0.001476958614426122	6.393760235610918E-6	4.262506823740612E-6	1.1508768424099651E-4	9.377515012229345E-5
34-35	0.0016069650725502105	8.525013647481224E-6	4.262506823740612E-6	1.1721893765286682E-4	9.377515012229345E-5
36-37	0.0018030403864422787	8.525013647481224E-6	4.262506823740612E-6	1.1935019106473713E-4	9.377515012229345E-5
38-39	0.002065184556102326	8.525013647481224E-6	4.262506823740612E-6	1.1935019106473713E-4	1.2361269788847774E-4
40-41	0.0024019225951778344	8.525013647481224E-6	4.262506823740612E-6	1.3213771153595895E-4	1.385314717715699E-4
42-43	0.002796204476373841	8.525013647481224E-6	4.262506823740612E-6	1.3640021835969958E-4	1.449252320071808E-4
44-45	0.0032821302542802707	8.525013647481224E-6	8.525013647481224E-6	1.4066272518344018E-4	1.491877388309214E-4
46-47	0.0037872373128935333	1.0656267059351529E-5	8.525013647481224E-6	1.4279397859531047E-4	1.513189922427917E-4
48-49	0.004496944699046345	1.2787520471221834E-5	8.525013647481224E-6	1.470564854190511E-4	1.6197525930214325E-4
50-51	0.005464533748035464	1.2787520471221834E-5	8.525013647481224E-6	1.491877388309214E-4	1.7050027294962446E-4
52-53	0.0065983605631504665	1.2787520471221834E-5	8.525013647481224E-6	1.491877388309214E-4	1.7476277977336506E-4
54-55	0.007917606425098186	1.2787520471221834E-5	8.525013647481224E-6	1.491877388309214E-4	1.7476277977336506E-4
56-57	0.009629002914830041	1.2787520471221834E-5	8.525013647481224E-6	1.513189922427917E-4	1.7476277977336506E-4
58-59	0.01163877488222374	1.2787520471221834E-5	8.525013647481224E-6	1.555814990665323E-4	1.7476277977336506E-4
60-61	0.014238904044705514	1.2787520471221834E-5	8.525013647481224E-6	1.5771275247840262E-4	1.8115654000897598E-4
62-63	0.01736119029309551	1.2787520471221834E-5	8.525013647481224E-6	1.6197525930214323E-4	1.918128070683275E-4
64-65	0.02134876542670485	1.2787520471221834E-5	8.525013647481224E-6	1.6197525930214323E-4	1.9607531389206814E-4
66-67	0.026382785985542515	1.2787520471221834E-5	8.525013647481224E-6	1.7476277977336506E-4	2.1099408777516026E-4
68-69	0.032522927065140865	1.491877388309214E-5	1.0656267059351529E-5	1.896815536564572E-4	2.2165035483451178E-4
70-71	0.040199701854697706	1.7050027294962447E-5	1.2787520471221834E-5	1.918128070683275E-4	2.259128616582524E-4
72-73	0.05024429918484245	1.9181280706832753E-5	1.2787520471221834E-5	2.0033782071580874E-4	2.3017536848199302E-4
74-75	0.0629593570400607	2.1312534118703058E-5	1.2787520471221834E-5	2.0460032753954934E-4	2.3870038212947422E-4
76-77	0.0787199760208416	2.1312534118703058E-5	1.2787520471221834E-5	2.0460032753954934E-4	2.4296288895321485E-4
78-79	0.0978479753923776	2.1312534118703058E-5	1.2787520471221834E-5	2.0460032753954934E-4	2.4296288895321485E-4
80-81	0.12213573927405161	2.1312534118703058E-5	1.2787520471221834E-5	2.1312534118703058E-4	2.4509414236508517E-4
82-83	0.15285136344592645	2.1312534118703058E-5	1.2787520471221834E-5	2.1312534118703058E-4	2.472253957769555E-4
84-85	0.19235627668835445	2.983754776618428E-5	1.2787520471221834E-5	2.1738784801077118E-4	2.5148790260069606E-4
86-87	0.24196333110304769	2.983754776618428E-5	1.2787520471221834E-5	2.3230662189386333E-4	2.5148790260069606E-4
88-89	0.3027317596357057	2.983754776618428E-5	1.2787520471221834E-5	2.3870038212947425E-4	2.5148790260069606E-4
90-91	0.3760063831892187	2.983754776618428E-5	1.2787520471221834E-5	2.3870038212947425E-4	2.5148790260069606E-4
92-93	0.46859229390768853	2.983754776618428E-5	1.2787520471221834E-5	2.3870038212947425E-4	2.5148790260069606E-4
94-95	0.5828423955578199	2.983754776618428E-5	1.2787520471221834E-5	2.4083163554134454E-4	2.642754230719179E-4
96-97	0.7209667979277227	3.8362561413665505E-5	1.491877388309214E-5	2.493566491888258E-4	2.6853792989565853E-4
98-99	0.8865332192288673	3.8362561413665505E-5	1.7050027294962447E-5	2.6001291624817727E-4	2.6853792989565853E-4
100-101	1.0849465181137572	3.8362561413665505E-5	1.7050027294962447E-5	2.7066918330752884E-4	2.6853792989565853E-4
102-103	1.3215795844337173	3.8362561413665505E-5	1.7050027294962447E-5	2.7280043671939916E-4	2.7280043671939916E-4
104-105	1.6033376792432072	3.8362561413665505E-5	1.7050027294962447E-5	2.7706294354313974E-4	2.7280043671939916E-4
106-107	1.9322902496051533	3.8362561413665505E-5	1.7050027294962447E-5	2.8558795719062094E-4	2.7280043671939916E-4
108-109	2.313403115969213	4.0493814825535814E-5	1.7050027294962447E-5	2.919817174262319E-4	2.7706294354313974E-4
110-111	2.7474136920158942	4.6887575061146725E-5	1.7050027294962447E-5	3.0690049130932404E-4	2.7706294354313974E-4
112-113	3.2380410149489096	4.6887575061146725E-5	1.7050027294962447E-5	3.090317447211943E-4	2.7706294354313974E-4
114-115	3.796970747225545	4.6887575061146725E-5	1.7050027294962447E-5	3.1755675836867556E-4	2.8558795719062094E-4
116-117	4.425366553208681	4.6887575061146725E-5	1.7050027294962447E-5	3.196880117805459E-4	2.8558795719062094E-4
118-119	5.120338453271821	4.9018828473017034E-5	1.7050027294962447E-5	3.196880117805459E-4	2.8558795719062094E-4
120-121	5.875637612411363	5.328133529675764E-5	1.7050027294962447E-5	3.196880117805459E-4	2.8558795719062094E-4
122-123	6.700925002343313	5.5412588708627946E-5	1.7050027294962447E-5	3.196880117805459E-4	2.8771921060249126E-4
124-125	7.594237738675339	5.5412588708627946E-5	1.7050027294962447E-5	3.282130254280271E-4	3.005067310737131E-4
126-127	8.547862815309884	5.5412588708627946E-5	1.7050027294962447E-5	3.282130254280271E-4	3.026379844855834E-4
128-129	9.566495383513296	5.5412588708627946E-5	1.7050027294962447E-5	3.282130254280271E-4	3.026379844855834E-4
130-131	10.632298983481634	5.5412588708627946E-5	1.7050027294962447E-5	3.282130254280271E-4	3.047692378974537E-4
132-133	11.742142803952861	5.967509553236856E-5	1.7050027294962447E-5	3.367380390755083E-4	3.111629981330646E-4
134-135	12.920210177391468	5.967509553236856E-5	1.7050027294962447E-5	3.367380390755083E-4	3.2395051860428645E-4
136-137	14.128984649988302	5.967509553236856E-5	1.7050027294962447E-5	3.367380390755083E-4	3.410005458992489E-4
138-139	15.368042102314401	6.180634894423887E-5	1.9181280706832753E-5	3.4313179931111924E-4	3.4739430613485987E-4
>>END_MODULE
