[2019-07-05 10:57:28] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-07-05 10:57:28] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-07-05 10:57:28] Checking for Bowtie index files (genome).. [2019-07-05 10:57:28] Checking for reference FASTA file [2019-07-05 10:57:28] Generating SAM header for Bowtie2Index/genome [2019-07-05 10:57:31] Reading known junctions from GTF file [2019-07-05 10:57:34] Preparing reads left reads: min. length=150, max. length=150, 21134222 kept reads (1698 discarded) right reads: min. length=150, max. length=150, 21121472 kept reads (14448 discarded) [2019-07-05 11:15:11] Building transcriptome data files /scratch/7534469.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-07-05 11:15:20] Building Bowtie index from RefSeq_GeneBody.fa [2019-07-05 11:18:52] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-07-05 11:31:49] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-07-05 11:45:51] Resuming TopHat pipeline with unmapped reads [2019-07-05 11:45:51] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-07-05 11:53:39] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2019-07-05 11:54:21] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2019-07-05 11:55:04] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2019-07-05 11:55:53] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2019-07-05 11:56:43] Mapping left_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2019-07-05 11:57:27] Mapping left_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2019-07-05 11:58:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-07-05 12:06:58] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/6) [2019-07-05 12:08:05] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/6) [2019-07-05 12:09:13] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/6) [2019-07-05 12:10:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/6) [2019-07-05 12:11:39] Mapping right_kept_reads.m2g_um_seg5 to genome genome with Bowtie2 (5/6) [2019-07-05 12:12:44] Mapping right_kept_reads.m2g_um_seg6 to genome genome with Bowtie2 (6/6) [2019-07-05 12:13:48] Searching for junctions via segment mapping [2019-07-05 13:24:28] Retrieving sequences for splices [2019-07-05 13:25:35] Indexing splices [2019-07-05 13:26:41] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2019-07-05 13:27:21] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2019-07-05 13:28:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2019-07-05 13:28:49] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2019-07-05 13:29:34] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2019-07-05 13:30:17] Mapping left_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2019-07-05 13:30:56] Joining segment hits [2019-07-05 13:33:59] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/6) [2019-07-05 13:36:03] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/6) [2019-07-05 13:38:03] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/6) [2019-07-05 13:40:01] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/6) [2019-07-05 13:41:55] Mapping right_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/6) [2019-07-05 13:43:33] Mapping right_kept_reads.m2g_um_seg6 to genome segment_juncs with Bowtie2 (6/6) [2019-07-05 13:45:09] Joining segment hits [2019-07-05 13:48:31] Reporting output tracks ----------------------------------------------- [2019-07-05 14:37:09] A summary of the alignment counts can be found in /scratch/7534469.1.linga/tophat2/align_summary.txt [2019-07-05 14:37:09] Run complete: 03:39:40 elapsed