##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR7518416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16298947
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.585891714354307	32.0	32.0	32.0	27.0	32.0
2	29.402714727521968	32.0	32.0	32.0	12.0	32.0
3	31.492985344390654	32.0	32.0	37.0	22.0	37.0
4	32.29685476000382	37.0	32.0	37.0	22.0	37.0
5	33.90383440108125	37.0	37.0	37.0	27.0	37.0
6	35.11539947948785	37.0	32.0	41.0	22.0	41.0
7	35.4354261658744	41.0	32.0	41.0	22.0	41.0
8	37.60294011631549	41.0	37.0	41.0	27.0	41.0
9	37.7302331248761	41.0	37.0	41.0	27.0	41.0
10-14	37.306415929814364	41.0	37.0	41.0	28.0	41.0
15-19	37.746631055368184	41.0	37.0	41.0	28.0	41.0
20-24	37.46154303097004	41.0	37.0	41.0	27.0	41.0
25-29	37.61574036654024	41.0	37.0	41.0	27.0	41.0
30-34	37.56964473839936	41.0	37.0	41.0	27.0	41.0
35-39	37.21699896318455	41.0	37.0	41.0	27.0	41.0
40-44	37.244432453213086	41.0	37.0	41.0	27.0	41.0
45-49	37.05559859787262	41.0	37.0	41.0	27.0	41.0
50-54	37.03759569253155	41.0	37.0	41.0	27.0	41.0
55-59	36.982339632124706	41.0	37.0	41.0	27.0	41.0
60-64	36.84498374036065	41.0	37.0	41.0	23.0	41.0
65-69	36.76411715431678	41.0	37.0	41.0	22.0	41.0
70-74	36.550031471358245	41.0	37.0	41.0	22.0	41.0
75-79	36.13271988675096	40.2	36.0	41.0	22.0	41.0
80-84	36.57104659583223	41.0	37.0	41.0	22.0	41.0
85-89	36.35872833993509	41.0	37.0	41.0	22.0	41.0
90-94	36.08830091907164	41.0	37.0	41.0	22.0	41.0
95-99	35.698452973679835	41.0	35.0	41.0	22.0	41.0
100-104	35.33686982355363	41.0	32.0	41.0	22.0	41.0
105-109	34.84643274194339	41.0	32.0	41.0	16.0	41.0
110-114	34.21773643413896	37.8	32.0	41.0	12.0	41.0
115-119	33.623716366462205	37.0	31.0	41.0	12.0	41.0
120-124	32.80622398489915	37.0	27.0	41.0	12.0	41.0
125-129	32.06848922203379	37.0	27.0	41.0	12.0	41.0
130-134	31.296019982149765	37.0	25.0	41.0	12.0	41.0
135-139	30.526811063315932	37.0	22.0	41.0	12.0	41.0
140-144	29.85400391816723	32.0	22.0	41.0	12.0	41.0
145-149	28.99951915912114	32.0	22.0	41.0	12.0	41.0
150	28.61290646567536	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	10.0
12	91.0
13	701.0
14	3482.0
15	16080.0
16	47679.0
17	84966.0
18	109840.0
19	125348.0
20	140455.0
21	156693.0
22	176698.0
23	197389.0
24	222210.0
25	246500.0
26	276981.0
27	309547.0
28	345659.0
29	389688.0
30	436937.0
31	494841.0
32	564045.0
33	648549.0
34	755796.0
35	905473.0
36	1119250.0
37	1457592.0
38	2033216.0
39	2867118.0
40	2166113.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.841655009851102	18.3831418505321	11.99335879607618	40.78184434354062
2	22.498913154527184	29.690452296457696	28.706587924955045	19.104046624060075
3	21.70931233816363	28.14644997226057	23.66175488698129	26.48248280259451
4	23.673401881163237	30.985709775046555	21.751489210758955	23.589399133031254
5	25.53968137386192	31.731661472935812	22.814360921987394	19.91429623121487
6	22.203501796103573	35.10871566553631	21.68776738395669	21.00001515440343
7	21.94359280616319	20.884478416029086	33.035182384626324	24.136746393181408
8	22.471967951242146	25.543679464319354	25.607905579511847	26.37644700492666
9	22.096964311219942	23.41496704318278	28.766124397076027	25.72194424852125
10-14	24.46328054390039	27.654467613302725	23.209861814591985	24.672390028204898
15-19	23.710486120522134	27.285210804253296	24.658405456599187	24.345897618625386
20-24	23.78559866239401	27.293160061511458	24.57014488383698	24.351096392257553
25-29	23.75513819223986	27.10853999160847	24.51127413031139	24.625047685840286
30-34	23.316938299527855	27.290235046203907	24.761894068767358	24.63093258550088
35-39	23.346348521938467	27.107007875265047	24.814683603595064	24.73195999920142
40-44	23.46580210672677	26.64752573066671	24.94494233257306	24.94172983003346
45-49	23.519448663399466	26.645821749699262	24.907532381919946	24.92719720498133
50-54	23.672805790619105	26.74138804846506	24.865784923403165	24.72002123751267
55-59	23.78733362020936	26.731021283390138	24.686374349258617	24.79527074714189
60-64	23.73720050790454	26.54577351127973	25.006869826355675	24.71015615446005
65-69	23.973644637617262	26.448231067585787	25.19642363100814	24.381700663788806
70-74	23.99703450402206	26.51470583796097	25.03170997579899	24.456549682217975
75-79	24.050706800787164	26.52563970478988	25.022222581077568	24.401430913345383
80-84	24.01774228214224	26.780365294478308	24.898083973173673	24.30380845020578
85-89	23.890189226042036	26.67624166279748	24.68729090384489	24.746278207315598
90-94	23.900902038679863	26.981266158495405	24.633931789945056	24.483900012879676
95-99	23.891666754098097	27.15260625112012	24.603959864495398	24.35176713028639
100-104	24.33391447990008	27.33706627263705	24.181371328948405	24.147647918514462
105-109	24.365582165946158	27.640998242260572	24.039465122301376	23.953954469491894
110-114	24.644453832212402	27.637325986822702	23.82591701164587	23.892303169319025
115-119	24.725938433052765	27.651760221395232	23.789154623794058	23.833146721757945
120-124	25.05337599502561	27.655408969150162	23.787162043348562	23.504052992475664
125-129	25.373022862315114	27.70703211540403	23.674454506055216	23.245490516225644
130-134	25.914543511819303	27.56664230186039	23.533065106946022	22.985749079374283
135-139	25.89583077146545	27.69211548761556	23.605013161655698	22.807040579263298
140-144	25.860895233018876	27.87359130847505	23.432979967013164	22.832533491492907
145-149	26.131569266602945	27.548605215602723	23.614231941791424	22.70559357600291
150	25.833346832008253	27.75170488264252	23.84460546318371	22.57034282216551
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	678.0
1	447.5
2	199.5
3	190.5
4	197.5
5	198.5
6	195.5
7	205.5
8	209.5
9	191.5
10	205.5
11	261.0
12	315.0
13	361.5
14	459.5
15	606.5
16	795.0
17	1152.0
18	1748.5
19	2653.5
20	4411.0
21	6848.5
22	10076.0
23	15040.0
24	21139.5
25	28227.0
26	38025.0
27	53958.5
28	67546.0
29	85397.0
30	110697.5
31	139997.5
32	174173.0
33	207856.0
34	246758.5
35	281494.0
36	310600.5
37	349773.0
38	376391.5
39	383206.0
40	397930.5
41	427378.5
42	455892.5
43	488935.5
44	517812.0
45	547346.0
46	585254.5
47	613291.5
48	644352.5
49	681391.5
50	720358.0
51	705523.5
52	667491.5
53	663079.5
54	641846.5
55	621795.0
56	586012.0
57	514380.0
58	460498.0
59	409726.0
60	348713.5
61	287777.5
62	237296.0
63	205565.0
64	167425.5
65	126271.0
66	99607.5
67	82713.5
68	70347.0
69	59824.0
70	50460.0
71	42958.5
72	38180.0
73	33710.0
74	30301.5
75	25773.5
76	20478.5
77	17111.0
78	14541.0
79	12372.0
80	10670.0
81	9292.0
82	7968.5
83	6496.0
84	5024.0
85	3903.5
86	3043.0
87	2288.5
88	1625.0
89	1069.5
90	754.0
91	542.0
92	378.5
93	273.0
94	202.0
95	167.0
96	142.5
97	127.0
98	108.5
99	133.5
100	276.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1853493971113594
2	0.010933221636956056
3	0.005208925460031252
4	0.026026221203124353
5	0.008675407067708116
6	3.3130974657442593E-4
7	0.00781645587288553
8	0.0017240377553224759
9	2.6382072412407993E-4
10-14	0.0048236244954965495
15-19	5.632265691765242E-4
20-24	0.0027940455294443254
25-29	0.0037290752586654832
30-34	6.68754858826156E-4
35-39	5.669077885829066E-4
40-44	7.804185141530922E-4
45-49	0.004963510832939085
50-54	0.010716029691979488
55-59	9.362568023566184E-4
60-64	8.356368052488298E-4
65-69	0.002759687481651422
70-74	0.001782937265824596
75-79	0.0012896538653693395
80-84	0.0029449755251060084
85-89	0.003217385761178314
90-94	0.0024529191977862128
95-99	0.0028222682115599247
100-104	0.002925342354938635
105-109	0.0038456472065342626
110-114	0.004109467930658342
115-119	0.0034689357539477855
120-124	0.002587897242686905
125-129	0.003451756730051334
130-134	0.003530289410720828
135-139	0.003789201842303064
140-144	0.004958602540397242
145-149	0.004649380110261111
150	0.006448269326846698
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.6298947E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.17709863625338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54496402920154	35.640520538148216
2	8.644968436690927	7.465293097966026
3	2.97066246746948	3.8479375911883675
4	1.4836433360876078	2.5623765865309864
5	0.8723564041682113	1.883290925436909
6	0.591233680838122	1.5316652972773648
7	0.4177584136893596	1.2626317363793158
8	0.30445255763059803	1.051630248870076
9	0.23143858360159994	0.899356189716095
>10	1.5674458151483512	13.230741814614822
>50	0.1881974062550194	5.689865391911815
>100	0.1595695789258561	13.869520297050897
>500	0.01610244759723561	4.746892612911245
>1k	0.006857444170730429	4.9618563842365075
>5k	2.4749062225470865E-4	0.6800586806692207
>10k+	1.0190790328135062E-4	0.6763626070921148
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC	22519	0.13816229968721291	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4541462709216738E-5	0.0	0.0	6.135365677304184E-6	0.0
2	2.4541462709216738E-5	0.0	0.0	6.135365677304184E-6	0.0
3	2.4541462709216738E-5	0.0	0.0	6.135365677304184E-6	0.0
4	3.68121940638251E-5	0.0	0.0	6.135365677304184E-6	0.0
5	4.9082925418433475E-5	0.0	0.0	6.135365677304184E-6	0.0
6	6.135365677304184E-5	0.0	0.0	1.2270731354608369E-5	0.0
7	6.748902245034603E-5	0.0	0.0	1.2270731354608369E-5	0.0
8	8.589511948225858E-5	0.0	0.0	1.840609703191255E-5	0.0
9	1.165719478687795E-4	0.0	0.0	1.840609703191255E-5	0.0
10-11	1.6872255612586506E-4	3.067682838652092E-6	0.0	1.840609703191255E-5	0.0
12-13	2.3927926141486318E-4	6.135365677304184E-6	0.0	2.1473779870564644E-5	6.135365677304184E-6
14-15	3.435804779290343E-4	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
16-17	4.3867864592724915E-4	9.203048515956277E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
18-19	5.583182766346808E-4	1.2270731354608369E-5	0.0	2.4541462709216738E-5	6.135365677304184E-6
20-21	6.718225416648081E-4	1.2270731354608369E-5	0.0	2.4541462709216738E-5	6.135365677304184E-6
22-23	7.853268066949355E-4	1.2270731354608369E-5	0.0	2.4541462709216738E-5	6.135365677304184E-6
24-25	9.203048515956276E-4	1.2270731354608369E-5	0.0	2.7609145547868828E-5	6.135365677304184E-6
26-27	0.0011258396017853178	1.2270731354608369E-5	0.0	4.294755974112929E-5	6.135365677304184E-6
28-29	0.0014080664229413104	1.2270731354608369E-5	0.0	4.9082925418433475E-5	6.135365677304184E-6
30-31	0.0018160682404820385	1.2270731354608369E-5	0.0	4.9082925418433475E-5	6.135365677304184E-6
32-33	0.0021903255467975937	1.2270731354608369E-5	0.0	4.9082925418433475E-5	6.135365677304184E-6
34-35	0.0027670499204641872	1.2270731354608369E-5	0.0	4.9082925418433475E-5	6.135365677304184E-6
36-37	0.0033529773426467365	1.2270731354608369E-5	0.0	6.442133961169394E-5	1.2270731354608369E-5
38-39	0.004107627320955151	1.2270731354608369E-5	0.0	6.748902245034603E-5	1.2270731354608369E-5
40-41	0.005037135221066735	1.2270731354608369E-5	0.0	6.748902245034603E-5	1.2270731354608369E-5
42-43	0.006279546770720833	1.2270731354608369E-5	0.0	6.748902245034603E-5	1.2270731354608369E-5
44-45	0.0077152223392100115	1.2270731354608369E-5	0.0	6.748902245034603E-5	1.840609703191255E-5
46-47	0.00925519912421336	1.2270731354608369E-5	0.0	7.66920709663023E-5	1.840609703191255E-5
48-49	0.011034455170631575	1.2270731354608369E-5	0.0	7.975975380495439E-5	1.840609703191255E-5
50-51	0.013473263027359988	1.2270731354608369E-5	0.0	8.282743664360648E-5	1.840609703191255E-5
52-53	0.01669126232510603	1.2270731354608369E-5	0.0	8.589511948225858E-5	2.4541462709216738E-5
54-55	0.020771280500513314	2.4541462709216738E-5	0.0	9.203048515956276E-5	2.4541462709216738E-5
56-57	0.025535391948940014	2.4541462709216738E-5	0.0	9.816585083686695E-5	2.4541462709216738E-5
58-59	0.0311584545921893	2.4541462709216738E-5	0.0	1.0123353367551903E-4	2.4541462709216738E-5
60-61	0.03827854646070081	2.4541462709216738E-5	0.0	1.0430121651417112E-4	3.067682838652092E-5
62-63	0.04718709742414648	2.4541462709216738E-5	0.0	1.1043658219147531E-4	3.067682838652092E-5
64-65	0.05922468488301729	2.4541462709216738E-5	0.0	1.165719478687795E-4	3.68121940638251E-5
66-67	0.07414589421022107	2.4541462709216738E-5	0.0	1.4111341057799625E-4	3.68121940638251E-5
68-69	0.0928096766005804	2.4541462709216738E-5	0.0	1.472487762553004E-4	3.68121940638251E-5
70-71	0.1157712826478913	2.4541462709216738E-5	0.0	1.503164590939525E-4	3.68121940638251E-5
72-73	0.14447865865199758	2.4541462709216738E-5	0.0	1.6872255612586506E-4	4.294755974112929E-5
74-75	0.1821866161047091	2.4541462709216738E-5	0.0	1.8406097031912553E-4	4.294755974112929E-5
76-77	0.23047501166793166	2.4541462709216738E-5	0.0	1.963317016737339E-4	4.294755974112929E-5
78-79	0.29096358188047367	2.4541462709216738E-5	0.0	2.0860243302834225E-4	4.294755974112929E-5
80-81	0.36388547063807253	2.4541462709216738E-5	0.0	2.2087316438295062E-4	4.9082925418433475E-5
82-83	0.45213350285757725	3.067682838652092E-5	0.0	2.2087316438295062E-4	5.5218291095737656E-5
84-85	0.5585974357729981	3.067682838652092E-5	0.0	2.2394084722160272E-4	5.5218291095737656E-5
86-87	0.68786345522812	3.3744511225173015E-5	0.0	2.362115785762111E-4	6.135365677304184E-5
88-89	0.8408334599775065	3.68121940638251E-5	0.0	2.4541462709216737E-4	6.748902245034603E-5
90-91	1.0183817396301738	3.68121940638251E-5	0.0	2.791591383173404E-4	7.055670528899812E-5
92-93	1.2253122855114507	3.68121940638251E-5	0.0	2.822268211559925E-4	7.975975380495439E-5
94-95	1.4674230181863894	3.68121940638251E-5	0.0	2.852945039946446E-4	7.975975380495439E-5
96-97	1.7439776937737144	3.68121940638251E-5	0.0	3.00632918187905E-4	7.975975380495439E-5
98-99	2.0590287213032843	3.68121940638251E-5	0.0	3.00632918187905E-4	7.975975380495439E-5
100-101	2.4104716703477838	3.68121940638251E-5	0.0	3.00632918187905E-4	8.282743664360648E-5
102-103	2.7959689665841605	3.68121940638251E-5	0.0	3.00632918187905E-4	8.589511948225858E-5
104-105	3.221990353119131	3.68121940638251E-5	0.0	3.2210669805846965E-4	8.589511948225858E-5
106-107	3.6808574198075497	3.68121940638251E-5	0.0	3.3130974657442593E-4	8.589511948225858E-5
108-109	4.178058251247765	3.9879876902477195E-5	0.0	3.497158436063385E-4	9.203048515956276E-5
110-111	4.703303839198937	4.9082925418433475E-5	0.0	3.589188921222948E-4	1.0430121651417112E-4
112-113	5.263603838947387	5.215060825708556E-5	0.0	3.6198657496094687E-4	1.0430121651417112E-4
114-115	5.855408941448794	6.135365677304184E-5	0.0	3.7425730631555524E-4	1.0430121651417112E-4
116-117	6.474501082800011	6.135365677304184E-5	0.0	3.895957205088157E-4	1.135042650301274E-4
118-119	7.120509073377562	6.135365677304184E-5	0.0	3.98798769024772E-4	1.2270731354608368E-4
120-121	7.771124723578769	6.135365677304184E-5	0.0	3.98798769024772E-4	1.3191036206203997E-4
122-123	8.436606364816083	6.135365677304184E-5	0.0	4.172048660566845E-4	1.3497804490069206E-4
124-125	9.118497041557347	6.135365677304184E-5	0.0	4.4174632876590124E-4	1.4111341057799625E-4
126-127	9.811732009435946	6.135365677304184E-5	0.0	4.540170601205096E-4	1.4111341057799625E-4
128-129	10.51247359722073	6.135365677304184E-5	0.0	4.66287791475118E-4	1.472487762553004E-4
130-131	11.21836275680877	6.135365677304184E-5	0.0	4.8162620566837846E-4	1.472487762553004E-4
132-133	11.932804616150971	6.135365677304184E-5	0.0	5.00032302700291E-4	1.503164590939525E-4
134-135	12.651559637564317	6.748902245034603E-5	0.0	5.123030340548994E-4	1.5645182477125669E-4
136-137	13.370360674220242	6.748902245034603E-5	0.0	5.276414482481599E-4	1.5951950760990878E-4
138-139	14.09362212172357	7.66920709663023E-5	0.0	5.368444967641161E-4	1.6258719044856087E-4
>>END_MODULE
