##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR7518416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16298947
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44160717867234	32.0	32.0	32.0	32.0	32.0
2	31.343521701125844	32.0	32.0	32.0	32.0	32.0
3	35.26170365484347	37.0	37.0	37.0	32.0	37.0
4	35.997569904362535	37.0	37.0	37.0	32.0	37.0
5	36.219153544090915	37.0	37.0	37.0	37.0	37.0
6	39.57305922891828	41.0	41.0	41.0	37.0	41.0
7	39.57494033203495	41.0	41.0	41.0	37.0	41.0
8	39.7048225876187	41.0	41.0	41.0	37.0	41.0
9	39.74495278744081	41.0	41.0	41.0	37.0	41.0
10-14	39.79338129021464	41.0	41.0	41.0	37.0	41.0
15-19	39.780862481484235	41.0	41.0	41.0	37.0	41.0
20-24	39.69708692224105	41.0	41.0	41.0	37.0	41.0
25-29	39.55591904188657	41.0	41.0	41.0	37.0	41.0
30-34	39.47691506696721	41.0	41.0	41.0	37.0	41.0
35-39	39.43666935048012	41.0	41.0	41.0	37.0	41.0
40-44	39.38135083205068	41.0	41.0	41.0	37.0	41.0
45-49	39.34370262078894	41.0	41.0	41.0	37.0	41.0
50-54	39.28480696329646	41.0	41.0	41.0	37.0	41.0
55-59	39.20057045402994	41.0	41.0	41.0	37.0	41.0
60-64	39.13635316440995	41.0	41.0	41.0	37.0	41.0
65-69	39.08278239078881	41.0	41.0	41.0	37.0	41.0
70-74	39.01012826166009	41.0	41.0	41.0	33.0	41.0
75-79	38.65403482813951	41.0	40.2	41.0	32.0	41.0
80-84	38.89383015970295	41.0	41.0	41.0	32.0	41.0
85-89	38.87172890371384	41.0	41.0	41.0	32.0	41.0
90-94	38.75758260947778	41.0	41.0	41.0	32.0	41.0
95-99	38.60156716872568	41.0	41.0	41.0	32.0	41.0
100-104	38.4915508590831	41.0	41.0	41.0	32.0	41.0
105-109	38.37344907005342	41.0	41.0	41.0	32.0	41.0
110-114	38.16013670085558	41.0	37.8	41.0	32.0	41.0
115-119	37.94181384846517	41.0	37.0	41.0	32.0	41.0
120-124	37.702020639738265	41.0	37.0	41.0	28.0	41.0
125-129	37.37651323119217	41.0	37.0	41.0	27.0	41.0
130-134	37.019487283442295	41.0	37.0	41.0	27.0	41.0
135-139	36.61084571905166	41.0	37.0	41.0	26.0	41.0
140-144	36.11357658872073	41.0	35.0	41.0	22.0	41.0
145-149	35.56633683145297	41.0	32.0	41.0	22.0	41.0
150	35.26596620014778	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	31.0
13	56.0
14	73.0
15	98.0
16	202.0
17	360.0
18	612.0
19	1274.0
20	2563.0
21	5417.0
22	10435.0
23	19160.0
24	32252.0
25	50643.0
26	74225.0
27	104655.0
28	141135.0
29	182779.0
30	230552.0
31	284824.0
32	343548.0
33	411802.0
34	488436.0
35	584113.0
36	708537.0
37	898752.0
38	1246221.0
39	2246418.0
40	8229774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63572723371943	8.5194190699693	9.341908101873308	40.50294559443796
2	24.479055162734458	11.10408735410185	37.13180116844309	27.285056314720602
3	24.341175390039815	12.52319709909489	23.139334506540404	39.99629300432489
4	26.9890250572702	19.817391905140642	23.46726508049858	29.726317957090576
5	28.51146324272163	23.23192077112389	27.396055539139795	20.860560447014684
6	25.104289016166454	27.80472295860294	27.274234342658932	19.816753682571672
7	20.022324141553437	22.567611269611465	41.10731815987867	16.30274642895642
8	21.410867830909567	24.632118872464584	33.85817500971075	20.0988382869151
9	20.65034524017907	22.036152896540592	36.916344820642664	20.397157042637673
10-14	23.620066989603682	26.659879316130052	29.06887788517872	20.651175809087544
15-19	23.873358181298393	25.252227345701495	28.94314114367474	21.931273329325375
20-24	23.68306104924119	25.57736284348129	28.391764703587004	22.347811403690518
25-29	24.121712393130778	25.772953306810347	27.67071684125526	22.434617458803615
30-34	23.917236862980147	26.04348983863301	27.289435858833862	22.74983743955298
35-39	24.127311393240593	25.936082274175927	27.279732298773222	22.656874033810258
40-44	24.17751761198588	25.422036879877787	27.672175444683234	22.728270063453103
45-49	23.94781218375312	25.536708047807316	27.679413549141422	22.836066219298143
50-54	23.834840830521838	25.465473440405734	27.76300655007602	22.936679178996407
55-59	24.391152363831285	25.280123407378014	27.470277471862254	22.85844675692845
60-64	23.892366505593145	25.24102895613945	27.647309052816695	23.21929548545071
65-69	24.265038168313612	25.64694463293752	27.244186403220837	22.84383079552803
70-74	24.269235745982495	25.542986101912273	27.24694934204298	22.94082881006225
75-79	24.042554321972638	25.473291920984316	27.32060717709584	23.163546579947205
80-84	24.201768651404223	25.52705833933482	27.114647961260335	23.156525048000624
85-89	24.29798421705153	25.05330569027806	27.403216533019233	23.245493559651184
90-94	24.404453177686047	25.34944981841817	27.155075784374112	23.091021219521675
95-99	24.68636604973997	25.309503522594618	27.061453990054478	22.942676437610935
100-104	24.589536299231973	25.276890620124547	26.808638314893102	23.324934765750378
105-109	24.373731128061767	25.268552316290915	26.827339715276448	23.53037684037087
110-114	24.652472482686957	25.46586296736355	26.656387379723135	23.22527717022636
115-119	24.523572061092448	25.55270981282546	26.43122141853264	23.492496707549453
120-124	24.699931997932932	25.612679088910962	26.01251902139311	23.674869891763
125-129	24.699924947644842	25.81730581506904	25.778103408504883	23.704665828781234
130-134	24.73674944807019	25.55172703594724	25.669249061449058	24.04227445453351
135-139	24.651235450675852	25.665238919593108	25.449522767725213	24.234002862005827
140-144	24.710446826551014	25.678916365686995	25.41476343300866	24.195873374753333
145-149	24.718789024697905	25.47380976814997	25.40222646111457	24.405174746037556
150	24.43915671070103	25.30951403091416	25.54877501834782	24.70255424003699
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	581.0
1	388.5
2	251.0
3	314.5
4	339.0
5	376.0
6	401.5
7	396.5
8	417.5
9	433.0
10	422.0
11	460.5
12	502.0
13	547.0
14	658.5
15	855.5
16	1013.5
17	1373.0
18	2323.5
19	3487.5
20	5220.0
21	7800.5
22	10775.0
23	14854.0
24	21814.0
25	30811.0
26	40961.5
27	57344.5
28	76361.5
29	100545.5
30	134799.0
31	176640.0
32	217521.5
33	245121.0
34	265841.0
35	300121.0
36	342468.5
37	400013.0
38	447355.0
39	448451.0
40	441048.0
41	456674.0
42	476529.5
43	502053.0
44	542341.5
45	600266.5
46	620348.0
47	629558.5
48	659904.0
49	667802.5
50	699200.5
51	703899.5
52	678492.5
53	672176.5
54	640627.5
55	589359.0
56	526008.5
57	463437.5
58	424091.0
59	376981.5
60	317518.5
61	259986.5
62	211845.5
63	165878.0
64	127329.5
65	101503.0
66	76726.0
67	58992.0
68	46415.5
69	36243.5
70	28878.5
71	22436.0
72	20575.0
73	17428.5
74	12962.5
75	10458.5
76	9582.0
77	8264.0
78	6361.5
79	5197.5
80	4792.5
81	4447.0
82	3619.5
83	3102.5
84	2518.5
85	1656.5
86	1163.0
87	808.5
88	543.5
89	341.5
90	209.0
91	109.5
92	45.5
93	26.5
94	14.5
95	9.5
96	6.5
97	7.5
98	7.0
99	7.0
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05225490947359973
2	0.011509946010622649
3	2.0860243302834228E-4
4	0.010068135076456167
5	0.0014908938595849166
6	6.748902245034603E-5
7	0.0
8	0.0
9	2.4541462709216738E-5
10-14	0.0
15-19	2.466417002276282E-4
20-24	1.4724877625530043E-5
25-29	4.761043765588047E-4
30-34	5.325497407900031E-4
35-39	2.4541462709216738E-6
40-44	4.613794989332747E-4
45-49	0.0010405580188707897
50-54	8.822655843963417E-4
55-59	9.104882665119409E-4
60-64	8.098682694041523E-5
65-69	5.337768139254639E-4
70-74	5.276414482481599E-4
75-79	8.417721709261341E-4
80-84	0.001233208501138141
85-89	0.001337509717652312
90-94	0.0027928184563088647
95-99	0.0024087445649096225
100-104	0.002781774798089717
105-109	0.0019412297002990438
110-114	0.0017228106821870147
115-119	0.002752325042838657
120-124	0.001992766771988399
125-129	0.002808770407069855
130-134	2.7486438234322743E-4
135-139	1.7424438523543884E-4
140-144	0.0017338543404061626
145-149	0.0027879101637670214
150	5.521829109573766E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.6298947E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.383136418051734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55120884419576	26.320706566567516
2	11.08988615481717	7.62610137043484
3	4.1122456064978	4.241757050182433
4	2.0450697121326225	2.8126364358672697
5	1.2229713433522813	2.1024795266924734
6	0.8495529152048529	1.7526176266705193
7	0.5834841912036112	1.4043411580751242
8	0.43116007689960045	1.1859708593645313
9	0.3519634594317	1.0891446875828603
>10	2.2595481676776807	15.016622896892656
>50	0.2579424105271238	6.21442674287348
>100	0.206708654447857	14.362313787229708
>500	0.024429952659837457	5.8267533160670055
>1k	0.013149042071608667	8.249147851288846
>5k	5.876487615122943E-4	1.316830202070256
>10k+	9.182011898629598E-5	0.4781499221405316
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	19227	0.11796467587752756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.067682838652092E-5	0.0	0.0	0.0	6.135365677304184E-6
2	3.067682838652092E-5	6.135365677304184E-6	0.0	6.135365677304184E-6	6.135365677304184E-6
3	4.294755974112929E-5	6.135365677304184E-6	0.0	1.840609703191255E-5	6.135365677304184E-6
4	8.589511948225858E-5	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
5	9.203048515956276E-5	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
6	1.2270731354608368E-4	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
7	1.4111341057799625E-4	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
8	1.6565487328721297E-4	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
9	2.0860243302834225E-4	6.135365677304184E-6	0.0	2.4541462709216738E-5	6.135365677304184E-6
10-11	2.791591383173404E-4	6.135365677304184E-6	0.0	2.7609145547868828E-5	1.2270731354608367E-5
12-13	3.7118962347690315E-4	6.135365677304184E-6	6.135365677304184E-6	3.067682838652092E-5	3.3744511225173015E-5
14-15	4.8162620566837846E-4	9.203048515956277E-6	6.135365677304184E-6	3.067682838652092E-5	3.9879876902477195E-5
16-17	5.736566908279412E-4	1.2270731354608369E-5	6.135365677304184E-6	3.681219406382511E-5	4.294755974112929E-5
18-19	6.902286386967207E-4	1.2270731354608369E-5	1.2270731354608369E-5	4.294755974112929E-5	4.294755974112929E-5
20-21	8.068005865655003E-4	1.840609703191255E-5	1.2270731354608369E-5	4.601524257978138E-5	4.294755974112929E-5
22-23	8.926957060477588E-4	1.840609703191255E-5	1.2270731354608369E-5	4.9082925418433475E-5	4.294755974112929E-5
24-25	9.816585083686695E-4	1.840609703191255E-5	1.2270731354608369E-5	6.442133961169394E-5	4.294755974112929E-5
26-27	0.0011166365532693616	1.840609703191255E-5	1.2270731354608369E-5	7.36243881276502E-5	4.294755974112929E-5
28-29	0.0013497804490069205	1.840609703191255E-5	1.2270731354608369E-5	7.975975380495439E-5	4.294755974112929E-5
30-31	0.0016228042216469567	1.840609703191255E-5	1.2270731354608369E-5	8.282743664360648E-5	4.601524257978138E-5
32-33	0.0019203694569962097	1.840609703191255E-5	1.2270731354608369E-5	8.589511948225858E-5	4.9082925418433475E-5
34-35	0.002426537125373805	3.067682838652092E-5	1.2270731354608369E-5	8.589511948225858E-5	6.135365677304184E-5
36-37	0.002929637110912748	3.067682838652092E-5	1.2270731354608369E-5	9.203048515956276E-5	6.135365677304184E-5
38-39	0.003601459652577556	3.067682838652092E-5	1.2270731354608369E-5	1.0736889935282322E-4	7.055670528899812E-5
40-41	0.00444200475036823	3.067682838652092E-5	1.2270731354608369E-5	1.165719478687795E-4	7.36243881276502E-5
42-43	0.005509558378219157	3.067682838652092E-5	1.533841419326046E-5	1.165719478687795E-4	7.975975380495439E-5
44-45	0.006840932730194165	3.68121940638251E-5	3.68121940638251E-5	1.165719478687795E-4	9.203048515956276E-5
46-47	0.00827047293300604	3.68121940638251E-5	3.68121940638251E-5	1.165719478687795E-4	9.203048515956276E-5
48-49	0.009939292397232779	3.68121940638251E-5	3.68121940638251E-5	1.165719478687795E-4	9.203048515956276E-5
50-51	0.012221648429189935	3.68121940638251E-5	3.68121940638251E-5	1.2270731354608368E-4	9.816585083686695E-5
52-53	0.015323075779067202	3.68121940638251E-5	3.9879876902477195E-5	1.2270731354608368E-4	1.0430121651417112E-4
54-55	0.019329469566346832	3.68121940638251E-5	4.294755974112929E-5	1.2884267922338787E-4	1.0430121651417112E-4
56-57	0.023961670652711493	4.294755974112929E-5	4.9082925418433475E-5	1.2884267922338787E-4	1.0430121651417112E-4
58-59	0.029434416836866824	4.9082925418433475E-5	4.9082925418433475E-5	1.3804572773934415E-4	1.0430121651417112E-4
60-61	0.03631522944396347	4.9082925418433475E-5	4.9082925418433475E-5	1.503164590939525E-4	1.0736889935282322E-4
62-63	0.045159359067797444	4.9082925418433475E-5	4.9082925418433475E-5	1.533841419326046E-4	1.1043658219147531E-4
64-65	0.05730124774318243	4.9082925418433475E-5	4.9082925418433475E-5	1.533841419326046E-4	1.1043658219147531E-4
66-67	0.07225313389877272	4.9082925418433475E-5	4.9082925418433475E-5	1.533841419326046E-4	1.165719478687795E-4
68-69	0.09104575896835543	4.9082925418433475E-5	4.9082925418433475E-5	1.533841419326046E-4	1.3497804490069206E-4
70-71	0.11409632781798726	4.9082925418433475E-5	5.215060825708556E-5	1.6258719044856087E-4	1.4724877625530043E-4
72-73	0.1430951336917655	4.9082925418433475E-5	5.5218291095737656E-5	1.8099328748047343E-4	1.533841419326046E-4
74-75	0.1809380691893777	4.9082925418433475E-5	5.5218291095737656E-5	1.9019633599642971E-4	1.5645182477125669E-4
76-77	0.22954857145065874	4.9082925418433475E-5	5.5218291095737656E-5	1.9019633599642971E-4	1.6565487328721297E-4
78-79	0.2907457763989293	4.9082925418433475E-5	5.5218291095737656E-5	1.932640188350818E-4	1.7179023896451715E-4
80-81	0.36443458586619126	4.9082925418433475E-5	7.36243881276502E-5	2.024670673510381E-4	1.7485792180316925E-4
82-83	0.45360599062013024	4.9082925418433475E-5	7.36243881276502E-5	2.0553475018969015E-4	1.7792560464182134E-4
84-85	0.561692727757198	4.9082925418433475E-5	7.36243881276502E-5	2.1473779870564644E-4	1.7792560464182134E-4
86-87	0.6934926532370465	5.5218291095737656E-5	7.66920709663023E-5	2.300762128989069E-4	1.99399384512386E-4
88-89	0.8491898280299948	5.828597393438975E-5	7.975975380495439E-5	2.3927926141486318E-4	2.024670673510381E-4
90-91	1.0296524063793815	6.135365677304184E-5	7.975975380495439E-5	2.5154999276947156E-4	2.024670673510381E-4
92-93	1.2407457978727092	6.135365677304184E-5	7.975975380495439E-5	2.5768535844677574E-4	2.024670673510381E-4
94-95	1.4886912633067646	6.135365677304184E-5	7.975975380495439E-5	2.6382072412407993E-4	2.024670673510381E-4
96-97	1.7716696667582268	6.135365677304184E-5	7.975975380495439E-5	2.6382072412407993E-4	2.0860243302834225E-4
98-99	2.0952918001389906	6.135365677304184E-5	7.975975380495439E-5	2.6382072412407993E-4	2.2087316438295062E-4
100-101	2.458005415932698	6.135365677304184E-5	7.975975380495439E-5	2.66888406962732E-4	2.2087316438295062E-4
102-103	2.85609861790458	6.135365677304184E-5	7.975975380495439E-5	2.852945039946446E-4	2.2394084722160272E-4
104-105	3.2963356467138647	6.135365677304184E-5	7.975975380495439E-5	2.944975525106008E-4	2.270085300602548E-4
106-107	3.7705840751552846	6.442133961169394E-5	7.975975380495439E-5	2.975652353492529E-4	2.270085300602548E-4
108-109	4.285546790231296	6.748902245034603E-5	7.975975380495439E-5	3.00632918187905E-4	2.270085300602548E-4
110-111	4.831625012339754	7.055670528899812E-5	7.975975380495439E-5	3.00632918187905E-4	2.270085300602548E-4
112-113	5.414889685818354	7.36243881276502E-5	7.975975380495439E-5	3.00632918187905E-4	2.33143895737559E-4
114-115	6.035285592375999	7.36243881276502E-5	7.975975380495439E-5	3.037006010265571E-4	2.33143895737559E-4
116-117	6.685508579173857	7.36243881276502E-5	7.975975380495439E-5	3.1290364954251337E-4	2.33143895737559E-4
118-119	7.3675679784712464	7.36243881276502E-5	7.975975380495439E-5	3.1903901521981756E-4	2.33143895737559E-4
120-121	8.056502055010057	7.36243881276502E-5	7.975975380495439E-5	3.1903901521981756E-4	2.33143895737559E-4
122-123	8.760884982324317	7.36243881276502E-5	7.975975380495439E-5	3.1903901521981756E-4	2.33143895737559E-4
124-125	9.484858132246213	7.36243881276502E-5	7.975975380495439E-5	3.1903901521981756E-4	2.6075304128542784E-4
126-127	10.221190976325035	7.36243881276502E-5	7.975975380495439E-5	3.374451122517301E-4	2.730237726400362E-4
128-129	10.965920068333249	7.36243881276502E-5	7.975975380495439E-5	3.435804779290343E-4	2.760914554786883E-4
130-131	11.720005592999351	7.66920709663023E-5	7.975975380495439E-5	3.435804779290343E-4	2.791591383173404E-4
132-133	12.487634937398102	7.975975380495439E-5	7.975975380495439E-5	3.497158436063385E-4	2.822268211559925E-4
134-135	13.26278930780007	1.0430121651417112E-4	7.975975380495439E-5	3.497158436063385E-4	2.883621868332967E-4
136-137	14.043536064016896	1.0430121651417112E-4	7.975975380495439E-5	3.497158436063385E-4	2.944975525106008E-4
138-139	14.832040990132676	1.0736889935282322E-4	8.282743664360648E-5	3.527835264449906E-4	2.975652353492529E-4
>>END_MODULE
