##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR7518413_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23525043
Sequences flagged as poor quality	0
Sequence length	150
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.5171846444659	32.0	27.0	32.0	11.0	32.0
2	29.664073005095037	32.0	32.0	32.0	27.0	32.0
3	32.49549269686776	37.0	32.0	37.0	27.0	37.0
4	33.267850137404636	37.0	32.0	37.0	27.0	37.0
5	34.63225431723972	37.0	37.0	37.0	27.0	37.0
6	38.195421534404844	42.0	37.0	42.0	32.0	42.0
7	38.13276154266753	42.0	37.0	42.0	32.0	42.0
8	38.33673447483178	42.0	37.0	42.0	32.0	42.0
9	39.24884120296826	42.0	37.0	42.0	32.0	42.0
10-14	38.49839884245908	42.0	37.0	42.0	32.0	42.0
15-19	39.111570584589366	42.0	38.0	42.0	32.0	42.0
20-24	38.98462299941386	42.0	38.0	42.0	32.0	42.0
25-29	39.169092009736175	42.0	40.0	42.0	32.0	42.0
30-34	38.878822733714024	42.0	38.0	42.0	32.0	42.0
35-39	38.54967341823775	42.0	37.0	42.0	32.0	42.0
40-44	38.82506245790922	42.0	37.0	42.0	32.0	42.0
45-49	38.479657138139984	42.0	37.0	42.0	32.0	42.0
50-54	38.44497097837398	42.0	37.0	42.0	32.0	42.0
55-59	38.39517422348601	42.0	37.0	42.0	32.0	42.0
60-64	38.355255707715386	42.0	37.0	42.0	32.0	42.0
65-69	38.30322103980852	42.0	37.0	42.0	32.0	42.0
70-74	38.203573876570594	42.0	37.0	42.0	28.0	42.0
75-79	37.749796461583514	42.0	37.0	42.0	28.0	42.0
80-84	38.22112905808503	42.0	37.0	42.0	31.0	42.0
85-89	38.06913362071219	42.0	37.0	42.0	27.0	42.0
90-94	37.81875146413123	42.0	37.0	42.0	27.0	42.0
95-99	37.40983151444186	42.0	37.0	42.0	27.0	42.0
100-104	36.974969261480204	42.0	37.0	42.0	25.0	42.0
105-109	36.615875405626255	42.0	37.0	42.0	22.0	42.0
110-114	36.109361763972124	42.0	32.0	42.0	22.0	42.0
115-119	35.4324606675533	40.0	32.0	42.0	22.0	42.0
120-124	34.70370812074604	37.0	32.0	42.0	22.0	42.0
125-129	34.026759389982836	37.0	30.0	42.0	11.0	42.0
130-134	33.29731022383253	37.0	27.0	42.0	11.0	42.0
135-139	32.51386466753748	37.0	27.0	42.0	11.0	42.0
140-144	31.607549138167354	35.0	27.0	42.0	11.0	42.0
145-149	30.821495918200874	32.0	26.0	42.0	11.0	42.0
150	30.436842304602802	32.0	22.0	42.0	11.0	42.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	11.0
5	15.0
6	19.0
7	27.0
8	42.0
9	94.0
10	441.0
11	1796.0
12	5381.0
13	12181.0
14	23509.0
15	40469.0
16	61160.0
17	83321.0
18	101396.0
19	115519.0
20	128497.0
21	141896.0
22	157673.0
23	176156.0
24	197600.0
25	222186.0
26	249505.0
27	283339.0
28	322905.0
29	368983.0
30	424183.0
31	493138.0
32	577449.0
33	691223.0
34	840309.0
35	1045346.0
36	1352473.0
37	1827422.0
38	2605253.0
39	3764171.0
40	4611965.0
41	2597978.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.14197924013406	17.10800689568591	15.162751864519572	35.587261999660456
2	24.37449146425137	28.275973476645827	26.39251860486273	20.957016454240073
3	24.14775248934595	27.475058078220506	22.757293063499876	25.61989636893367
4	26.130046128391715	29.283624380557683	21.51035892000979	23.075970571040813
5	27.733713198862002	29.82976359942624	21.91305555801159	20.523467643700165
6	23.929565621737623	33.94733876592207	20.47699920666765	21.64609640567266
7	23.39685043902081	20.33017667061046	31.248168151833696	25.02480473853504
8	24.975035965170946	24.58483144818437	23.97035763426774	26.46977495237694
9	24.060953016067124	22.960782506705264	26.983918423478737	25.994346053748878
10-14	26.251297893690595	26.30817609474724	22.356914579912633	25.083611431649537
15-19	25.71441474539758	26.050292907303824	23.731259144931276	24.50403320236732
20-24	25.631090621805875	26.03800562119622	23.74923181116818	24.58167194582973
25-29	25.6705958602072	25.96152784260432	23.577484754285898	24.79039154290258
30-34	25.445803210081692	26.1628066532054	23.6251377956826	24.766252341030302
35-39	25.312243281531995	26.208755139300326	23.613605925349173	24.865395653818506
40-44	25.451836452119252	25.769258131522438	23.760286763304105	25.018618653054205
45-49	25.52732092963214	25.83646240485855	23.7607654218598	24.8754512436495
50-54	25.471555838344205	25.95284971696494	23.651376959362693	24.924217485328157
55-59	25.630841219423324	25.78684922632048	23.639768659296166	24.94254089496003
60-64	25.6989198715117	25.72864515224234	23.841939143872466	24.730495832373496
65-69	25.87077680524848	25.638747230908344	23.95758172334808	24.5328942404951
70-74	25.825527798328913	25.67689474746622	23.794957059758737	24.70262039444612
75-79	25.652522635467324	25.821247971623258	23.701776176415148	24.824453216494273
80-84	25.77434724846317	26.100022170304637	23.594431680642586	24.531198900589608
85-89	25.742264064548355	26.032511984423383	23.4956615820216	24.72956236900666
90-94	25.929584162271585	26.271193379859454	23.389036991814528	24.410185466054436
95-99	26.030618716606384	26.327247067293342	23.293592201152123	24.348542014948148
100-104	26.53877752419386	26.629899890131774	22.85802963802801	23.97329294764635
105-109	26.86898142870009	26.88812501241433	22.599148526319883	23.64374503256569
110-114	27.364157021439446	26.761617529410977	22.455112675430023	23.419112773719554
115-119	27.863820140564187	26.666713372267154	22.367393412328656	23.102073074839996
120-124	28.315453050204276	26.848533970281945	22.272655766672532	22.56335721284125
125-129	28.99436818487472	26.92089623000678	22.0326967876484	22.052038797470097
130-134	29.52212496611308	26.93028866276654	21.973465232072222	21.57412113904816
135-139	29.642556333255833	26.9599632170424	22.1720497659521	21.225430683749668
140-144	29.90886283146368	27.075749306273188	22.1040222981501	20.911365564113034
145-149	30.213195713334972	26.772711907693804	22.34735480746701	20.666737571504214
150	29.999747920996484	27.02324750787173	22.542787647754746	20.43421692337704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4853.0
1	2839.5
2	896.0
3	1063.0
4	1134.0
5	1012.0
6	885.0
7	863.0
8	823.0
9	730.5
10	673.0
11	679.0
12	726.5
13	751.5
14	768.0
15	840.0
16	958.0
17	1132.5
18	1398.0
19	1838.5
20	2647.5
21	3659.0
22	5116.5
23	7242.5
24	10221.0
25	14223.5
26	20049.0
27	30454.5
28	41756.0
29	57062.5
30	77356.5
31	100066.0
32	127874.5
33	171810.0
34	228736.5
35	264233.5
36	292539.5
37	350921.0
38	404193.0
39	423503.5
40	439451.0
41	479473.0
42	539278.0
43	617879.5
44	675431.5
45	744011.0
46	845195.5
47	912248.5
48	982188.5
49	1063767.5
50	1144855.0
51	1146295.0
52	1107766.5
53	1097438.0
54	1047185.5
55	1011871.5
56	967126.5
57	881161.5
58	804701.0
59	708923.0
60	596457.5
61	499484.5
62	421254.5
63	357030.0
64	290068.0
65	231925.0
66	188492.0
67	158348.0
68	133280.0
69	107344.0
70	89038.5
71	77367.0
72	72147.0
73	66299.5
74	58918.0
75	48114.5
76	37437.5
77	33707.0
78	29444.5
79	24623.5
80	22109.5
81	21321.5
82	20224.5
83	17865.0
84	14676.0
85	11752.0
86	8830.0
87	5692.5
88	3334.5
89	1951.0
90	1380.5
91	890.5
92	417.0
93	267.0
94	177.5
95	132.0
96	118.0
97	95.5
98	97.0
99	152.0
100	262.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047655598334081686
2	0.006899030960325981
3	0.00678000886119528
4	2.2104104124273015E-4
5	9.649291608096104E-4
6	9.521767930456067E-4
7	0.0012922399334190377
8	8.501578509335774E-4
9	9.989354748469537E-4
10-14	0.0016535570200658083
15-19	0.0011162572582757873
20-24	9.700301079152119E-4
25-29	0.0012769370921022333
30-34	0.0015226327110220374
35-39	0.0010431436830954995
40-44	0.0010533455773067025
45-49	0.001189370833456075
50-54	0.001229328252449953
55-59	9.55577424449341E-4
60-64	0.0012165758846859494
65-69	0.0023030776181790615
70-74	0.006832718647953161
75-79	0.01236469578397795
80-84	0.014834404340939992
85-89	0.014569155091448717
90-94	0.014826752920281593
95-99	0.01224822415840005
100-104	0.012948754227569319
105-109	0.01634938563130363
110-114	0.011481381776857964
115-119	0.0076990294980544775
120-124	0.010879470018396991
125-129	0.004749831913165897
130-134	0.0051834124171420215
135-139	0.0028497291163293516
140-144	0.0018567447464389332
145-149	0.0023591880363406778
150	0.0028565303791368204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3525043E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.50404166810849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.830341770285	25.406229303785366
2	10.932748461000376	7.32582519968611
3	4.1382702365699515	4.159463353197985
4	2.184598579569704	2.9277152735197594
5	1.3300530207510783	2.2281075914018853
6	0.9118933931761836	1.8331268545108617
7	0.6608275613081871	1.5498275904652814
8	0.49278525631541836	1.3208238208817038
9	0.3759353161779336	1.1335817247965123
>10	2.589803448744184	17.02798102425182
>50	0.3053696682215212	7.144251789200437
>100	0.21428784141784107	14.018774077513108
>500	0.020743072301346414	4.804874864000368
>1k	0.011698832741367663	7.26849300969184
>5k	4.826560649218628E-4	1.072132586766628
>10k+	1.6088535497394717E-4	0.7787919363300004
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT	24416	0.10378727044197114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.975552478267521E-5	0.0	0.0	4.250789254667887E-6	0.0
2	3.40063140373431E-5	0.0	0.0	8.501578509335775E-6	0.0
3	3.40063140373431E-5	4.250789254667887E-6	0.0	8.501578509335775E-6	0.0
4	3.40063140373431E-5	4.250789254667887E-6	0.0	8.501578509335775E-6	0.0
5	4.675868180134676E-5	4.250789254667887E-6	0.0	1.2752367764003662E-5	0.0
6	6.80126280746862E-5	4.250789254667887E-6	0.0	1.2752367764003662E-5	0.0
7	9.351736360269352E-5	4.250789254667887E-6	0.0	2.1253946273339435E-5	0.0
8	1.0626973136669718E-4	4.250789254667887E-6	0.0	2.1253946273339435E-5	0.0
9	1.3177446689470451E-4	8.501578509335775E-6	0.0	2.1253946273339435E-5	0.0
10-11	1.8703472720538704E-4	8.501578509335775E-6	0.0	2.337934090067338E-5	0.0
12-13	2.9968064245408606E-4	8.501578509335775E-6	0.0	3.188091941000916E-5	0.0
14-15	4.272043200941227E-4	8.501578509335775E-6	0.0	3.825710329201098E-5	0.0
16-17	6.0361207416284E-4	8.501578509335775E-6	0.0	4.250789254667887E-5	0.0
18-19	7.800198282315573E-4	8.501578509335775E-6	0.0	4.250789254667887E-5	0.0
20-21	0.001058446524412304	8.501578509335775E-6	0.0	4.250789254667887E-5	0.0
22-23	0.0014941524230157625	8.501578509335775E-6	6.376183882001831E-6	4.675868180134676E-5	0.0
24-25	0.0023230563276760005	8.501578509335775E-6	8.501578509335775E-6	5.100947105601465E-5	0.0
26-27	0.0034686440318089963	8.501578509335775E-6	8.501578509335775E-6	5.526026031068254E-5	2.1253946273339437E-6
28-29	0.005007429741998771	8.501578509335775E-6	8.501578509335775E-6	5.738565493801648E-5	4.250789254667887E-6
30-31	0.007018053059456682	8.501578509335775E-6	8.501578509335775E-6	6.376183882001832E-5	8.501578509335775E-6
32-33	0.009662043975860107	8.501578509335775E-6	8.501578509335775E-6	6.376183882001832E-5	8.501578509335775E-6
34-35	0.012656725005773636	8.501578509335775E-6	8.501578509335775E-6	6.80126280746862E-5	8.501578509335775E-6
36-37	0.015891575628575898	8.501578509335775E-6	8.501578509335775E-6	6.80126280746862E-5	1.062697313666972E-5
38-39	0.01969178122224899	8.501578509335775E-6	8.501578509335775E-6	7.226341732935408E-5	1.2752367764003662E-5
40-41	0.024216746383842955	8.501578509335775E-6	8.501578509335775E-6	7.651420658402198E-5	1.700315701867155E-5
42-43	0.029645004262053846	8.501578509335775E-6	8.501578509335775E-6	7.651420658402198E-5	1.700315701867155E-5
44-45	0.03582565183834095	8.501578509335775E-6	8.501578509335775E-6	7.651420658402198E-5	1.700315701867155E-5
46-47	0.04319014422205307	8.501578509335775E-6	8.501578509335775E-6	7.651420658402198E-5	1.700315701867155E-5
48-49	0.05128364696294073	8.501578509335775E-6	8.501578509335775E-6	7.863960121135592E-5	1.700315701867155E-5
50-51	0.06078416094712345	8.501578509335775E-6	8.501578509335775E-6	8.076499583868986E-5	2.1253946273339435E-5
52-53	0.07243557429416814	8.501578509335775E-6	8.501578509335775E-6	8.71411797206917E-5	2.1253946273339435E-5
54-55	0.08674373092538024	8.501578509335775E-6	8.501578509335775E-6	9.351736360269352E-5	2.1253946273339435E-5
56-57	0.10383615451839981	1.062697313666972E-5	8.501578509335775E-6	1.020189421120293E-4	2.1253946273339435E-5
58-59	0.12348117705884747	1.2752367764003662E-5	8.501578509335775E-6	1.1477130987603296E-4	2.337934090067338E-5
60-61	0.14681163388309215	1.2752367764003662E-5	1.062697313666972E-5	1.1477130987603296E-4	2.763013015534127E-5
62-63	0.17514952044933563	1.2752367764003662E-5	1.2752367764003662E-5	1.1902209913070084E-4	2.975552478267521E-5
64-65	0.21031629995320306	1.2752367764003662E-5	1.2752367764003662E-5	1.1902209913070084E-4	2.975552478267521E-5
66-67	0.25317063182413735	1.2752367764003662E-5	1.2752367764003662E-5	1.1902209913070084E-4	2.975552478267521E-5
68-69	0.30497074968152027	1.700315701867155E-5	1.700315701867155E-5	1.1902209913070084E-4	2.975552478267521E-5
70-71	0.36762525790069756	1.700315701867155E-5	2.1253946273339435E-5	1.1902209913070084E-4	3.40063140373431E-5
72-73	0.4443264992119249	1.700315701867155E-5	2.1253946273339435E-5	1.1902209913070084E-4	3.825710329201098E-5
74-75	0.5403241983447171	1.700315701867155E-5	2.1253946273339435E-5	1.2752367764003663E-4	4.250789254667887E-5
76-77	0.6565237734103185	1.700315701867155E-5	2.1253946273339435E-5	1.360252561493724E-4	4.250789254667887E-5
78-79	0.7977945034999512	2.1253946273339435E-5	2.1253946273339435E-5	1.4452683465870816E-4	4.250789254667887E-5
80-81	0.9667612509783723	2.1253946273339435E-5	2.1253946273339435E-5	1.4877762391337604E-4	4.250789254667887E-5
82-83	1.1700339931365906	2.1253946273339435E-5	2.1253946273339435E-5	1.5090301854071E-4	4.250789254667887E-5
84-85	1.4123183536795236	2.1253946273339435E-5	2.1253946273339435E-5	1.551538077953779E-4	4.463328717401282E-5
86-87	1.7023539553147682	2.5504735528007324E-5	2.1253946273339435E-5	1.5940459705004578E-4	4.88840764286807E-5
88-89	2.0437093356216183	2.5504735528007324E-5	2.1253946273339435E-5	1.6365538630471366E-4	5.100947105601465E-5
90-91	2.437366426917902	3.40063140373431E-5	2.1253946273339435E-5	1.7853314869605127E-4	5.100947105601465E-5
92-93	2.8918480616592284	3.40063140373431E-5	2.1253946273339435E-5	1.8278393795071916E-4	5.100947105601465E-5
94-95	3.4195134096035447	3.613170866467704E-5	2.1253946273339435E-5	1.849093325780531E-4	5.526026031068254E-5
96-97	4.0219926484300155	3.825710329201099E-5	2.1253946273339435E-5	1.8916012183272098E-4	5.526026031068254E-5
98-99	4.7001529391465935	3.825710329201099E-5	2.1253946273339435E-5	1.955363057147228E-4	5.526026031068254E-5
100-101	5.458714783220588	3.825710329201099E-5	2.1253946273339435E-5	1.997870949693907E-4	6.163644419268437E-5
102-103	6.287916243128652	3.825710329201099E-5	2.1253946273339435E-5	1.997870949693907E-4	6.376183882001832E-5
104-105	7.198826374089943	3.825710329201099E-5	2.1253946273339435E-5	1.997870949693907E-4	6.376183882001832E-5
106-107	8.179770383416514	3.825710329201099E-5	2.1253946273339435E-5	1.997870949693907E-4	6.376183882001832E-5
108-109	9.233266863741758	4.463328717401282E-5	2.1253946273339435E-5	2.0616327885139254E-4	6.376183882001832E-5
110-111	10.34603634943409	5.31348656833486E-5	2.5504735528007324E-5	2.3166801437939986E-4	6.588723344735226E-5
112-113	11.523528352317996	5.526026031068254E-5	2.5504735528007324E-5	2.3804419826140168E-4	6.80126280746862E-5
114-115	12.753915051292362	5.526026031068254E-5	2.5504735528007324E-5	2.3804419826140168E-4	7.226341732935408E-5
116-117	14.031224087454378	5.738565493801648E-5	2.5504735528007324E-5	2.4654577677073745E-4	7.438881195668802E-5
118-119	15.342932635659794	5.951104956535042E-5	2.5504735528007324E-5	2.486711713980714E-4	7.651420658402198E-5
120-121	16.6737527323542	5.951104956535042E-5	2.5504735528007324E-5	2.571727499074072E-4	7.651420658402198E-5
122-123	18.015416592437262	5.951104956535042E-5	2.5504735528007324E-5	2.6354893378940903E-4	7.651420658402198E-5
124-125	19.373093175642655	5.951104956535042E-5	2.975552478267521E-5	2.6354893378940903E-4	7.651420658402198E-5
126-127	20.733970603156813	5.951104956535042E-5	2.975552478267521E-5	2.6567432841674294E-4	7.651420658402198E-5
128-129	22.11182568295412	5.951104956535042E-5	2.975552478267521E-5	2.720505122987448E-4	7.651420658402198E-5
130-131	23.493801902933825	5.951104956535042E-5	2.975552478267521E-5	2.890536693174163E-4	7.651420658402198E-5
132-133	24.87829459015229	5.951104956535042E-5	2.975552478267521E-5	2.975552478267521E-4	8.28903904660238E-5
134-135	26.241431312155306	5.951104956535042E-5	2.975552478267521E-5	3.081822209634218E-4	8.501578509335774E-5
136-137	27.583016532637156	6.376183882001832E-5	2.975552478267521E-5	3.2305998335475944E-4	8.501578509335774E-5
138-139	28.90601092631372	6.588723344735226E-5	3.188091941000916E-5	3.358123511187631E-4	8.71411797206917E-5
>>END_MODULE
