##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR7518413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23525043
Sequences flagged as poor quality	0
Sequence length	150
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.977956257083143	32.0	32.0	32.0	27.0	32.0
2	31.57499346547422	32.0	32.0	32.0	32.0	32.0
3	35.6188707072714	37.0	37.0	37.0	32.0	37.0
4	36.285348341339905	37.0	37.0	37.0	37.0	37.0
5	36.51644220161468	37.0	37.0	37.0	37.0	37.0
6	40.71122981581798	42.0	42.0	42.0	37.0	42.0
7	40.82239003771428	42.0	42.0	42.0	37.0	42.0
8	40.961687593939786	42.0	42.0	42.0	37.0	42.0
9	41.008980302395194	42.0	42.0	42.0	37.0	42.0
10-14	41.079628793877234	42.0	42.0	42.0	37.0	42.0
15-19	41.040685817237396	42.0	42.0	42.0	37.0	42.0
20-24	40.97077637647676	42.0	42.0	42.0	37.0	42.0
25-29	40.88221114834944	42.0	42.0	42.0	37.0	42.0
30-34	40.833424015420505	42.0	42.0	42.0	37.0	42.0
35-39	40.795816934319745	42.0	42.0	42.0	37.0	42.0
40-44	40.75014354702774	42.0	42.0	42.0	37.0	42.0
45-49	40.69428514115786	42.0	42.0	42.0	37.0	42.0
50-54	40.6538657038799	42.0	42.0	42.0	37.0	42.0
55-59	40.590795612998456	42.0	42.0	42.0	37.0	42.0
60-64	40.540077261495334	42.0	42.0	42.0	37.0	42.0
65-69	40.46697716981856	42.0	42.0	42.0	37.0	42.0
70-74	40.364959494441734	42.0	42.0	42.0	37.0	42.0
75-79	39.96689101907274	42.0	41.0	42.0	36.0	42.0
80-84	40.29382780724354	42.0	42.0	42.0	37.0	42.0
85-89	40.2484028700819	42.0	42.0	42.0	37.0	42.0
90-94	40.17354806960395	42.0	42.0	42.0	37.0	42.0
95-99	40.08536418828224	42.0	42.0	42.0	37.0	42.0
100-104	39.97002646923961	42.0	42.0	42.0	37.0	42.0
105-109	39.82322729867061	42.0	42.0	42.0	37.0	42.0
110-114	39.72095665032366	42.0	42.0	42.0	35.0	42.0
115-119	39.504756781953596	42.0	42.0	42.0	32.0	42.0
120-124	39.18509209951285	42.0	39.0	42.0	32.0	42.0
125-129	38.81051744730074	42.0	37.0	42.0	32.0	42.0
130-134	38.51061939397943	42.0	37.0	42.0	32.0	42.0
135-139	38.116289504763074	42.0	37.0	42.0	30.0	42.0
140-144	37.57588861367862	42.0	37.0	42.0	27.0	42.0
145-149	36.86863637188676	42.0	36.0	42.0	27.0	42.0
150	36.45508082599466	42.0	32.0	42.0	22.0	42.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	24.0
12	68.0
13	95.0
14	157.0
15	228.0
16	341.0
17	666.0
18	1159.0
19	2155.0
20	3867.0
21	6762.0
22	11208.0
23	17990.0
24	28137.0
25	42107.0
26	56625.0
27	77442.0
28	105210.0
29	138343.0
30	178458.0
31	225850.0
32	282458.0
33	347719.0
34	425099.0
35	522419.0
36	651132.0
37	839646.0
38	1159186.0
39	1836511.0
40	4359533.0
41	1.2204447E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25508260049669	8.820488345341802	10.178289235002056	36.74613981915945
2	25.56852089624154	10.770137836064544	33.06499109339828	30.59635017429564
3	24.67491449369128	12.579404180648496	21.210032230118454	41.53564909554177
4	27.347276860818543	18.66525013329416	21.786826520187834	32.200646485699465
5	29.024495300603704	22.39828424543156	24.933038379568533	23.64418207439621
6	27.25806579137649	25.984025570241776	24.419101404532043	22.338807233849693
7	22.287750140457955	22.16349421056464	36.79883721172431	18.749918437253093
8	23.105802612135502	24.328729175967926	30.30506681751868	22.260401394377897
9	22.07747292959252	21.95976432434151	33.417188652960164	22.545574093105802
10-14	24.936786767713073	24.96017807386324	26.905420143276125	23.19761501514756
15-19	24.964325038640737	23.78349319063944	27.000071370751588	24.252110399968238
20-24	24.549070506630436	24.119978806923587	26.800921166497925	24.530029519948048
25-29	24.894300718261004	24.21267641881662	26.231015889043103	24.662006973879272
30-34	24.719952580897036	24.145627369938147	26.123069799797694	25.011350249367126
35-39	24.78798016020292	24.110037819703507	26.12230058364622	24.979681436447354
40-44	24.81239757988965	24.078485212545626	26.16303315577361	24.946084051791107
45-49	24.716611144982817	23.807720989075342	26.311558282805265	25.16410958313657
50-54	24.513363057402277	23.9832097225072	26.24410505859649	25.25932216149403
55-59	25.016290299799156	23.81638855084251	25.886244083687703	25.281077065670633
60-64	24.678708557514646	23.893128696937982	26.040685239130063	25.387477506417312
65-69	24.68937333626751	24.018173655892884	26.020387382109135	25.272065625730473
70-74	24.859476334175145	23.984572014135917	25.778471544427916	25.377480107261025
75-79	24.760853245690406	23.908087554636165	25.829512374984336	25.501546824689097
80-84	24.9363063408618	24.05545376183258	25.71268719777239	25.295552699533232
85-89	24.912838966670524	23.74628519966279	25.876053475220512	25.464822358446177
90-94	25.266174488082232	24.070343911280027	25.48822301168463	25.17525858895311
95-99	25.330754418530738	24.262900634223016	25.32828896015518	25.078055987091062
100-104	25.188464905250118	24.362466840124373	25.067292756914405	25.3817754977111
105-109	25.180910672033157	24.592304548946146	24.727156629680085	25.499628149340616
110-114	25.444156661281692	24.919582877852672	24.470811969575198	25.165448491290437
115-119	25.253624223380704	25.3930441776082	23.944829849386014	25.408501749625078
120-124	25.27360237456666	25.440505431971033	23.64757450248721	25.638317690975093
125-129	24.91847219864333	25.883692129849745	23.45562256282588	25.742213108681046
130-134	24.60514439867336	25.83229539686707	23.4104090691779	26.152151135281663
135-139	24.346567949270803	26.107152430832443	23.11363741029675	26.432642209600004
140-144	23.969350168832467	26.41214169248094	23.127766781030214	26.49074135765638
145-149	23.670117588944013	26.365648564311584	23.306976787128917	26.657257059615485
150	23.383052600811975	26.244422714262903	23.385938906223725	26.986585778701393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5013.0
1	2921.0
2	896.0
3	1078.5
4	1186.5
5	1122.0
6	1013.0
7	955.5
8	907.0
9	797.0
10	709.5
11	685.0
12	698.0
13	698.0
14	713.5
15	754.5
16	811.0
17	959.0
18	1286.5
19	1572.0
20	2135.5
21	3153.0
22	4413.5
23	6304.0
24	9051.0
25	12781.5
26	17563.5
27	26057.0
28	37592.5
29	56976.0
30	83606.5
31	116538.5
32	146898.0
33	172452.0
34	205868.0
35	244546.0
36	291358.0
37	369271.5
38	442197.5
39	446946.5
40	439357.0
41	477132.5
42	534423.5
43	600433.0
44	665043.5
45	760442.5
46	837569.5
47	903039.5
48	971771.5
49	1013325.5
50	1092859.5
51	1128440.0
52	1124182.0
53	1122149.0
54	1077694.5
55	1033748.0
56	964666.5
57	890055.0
58	837364.0
59	751733.0
60	642961.0
61	535956.5
62	446890.5
63	358732.0
64	285172.5
65	237687.5
66	191988.0
67	157779.0
68	124878.5
69	94527.5
70	76790.5
71	63157.5
72	59162.0
73	51531.5
74	38753.5
75	30840.5
76	29133.0
77	26515.0
78	20631.0
79	16481.0
80	15336.5
81	15683.0
82	14126.5
83	13088.0
84	11018.0
85	7451.0
86	6012.5
87	4140.0
88	2180.0
89	1259.5
90	853.5
91	460.0
92	185.5
93	89.0
94	48.5
95	35.0
96	26.0
97	20.5
98	15.5
99	10.0
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032947867512930794
2	0.0015217825531711037
3	0.0019681154249112315
4	2.5504735528007324E-5
5	0.0
6	8.374054831695737E-4
7	8.926657434802564E-4
8	0.0
9	0.0
10-14	2.6950003874594405E-4
15-19	0.0
20-24	6.801262807468619E-6
25-29	5.951104956535042E-6
30-34	3.910726114294456E-5
35-39	1.700315701867155E-5
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.501578509335774E-7
60-64	0.0
65-69	8.501578509335775E-6
70-74	8.501578509335775E-6
75-79	4.760883965228034E-5
80-84	7.141325947842051E-5
85-89	1.0031862641016214E-4
90-94	2.040378842240586E-5
95-99	2.465457767707375E-5
100-104	0.0
105-109	3.1455840484542366E-5
110-114	3.3071140401316166E-4
115-119	1.2752367764003662E-5
120-124	4.080757684481172E-5
125-129	1.7003157018671548E-6
130-134	0.0
135-139	1.3602525614937238E-5
140-144	2.7205051229874477E-5
145-149	3.485647188827668E-5
150	6.75875491492194E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3525043E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.398287758776775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51603373290394	22.34761548296754
2	11.90378304846676	7.237092450506832
3	4.576276904536444	4.173329466238292
4	2.387940231629011	2.9035717724727497
5	1.5051782616572589	2.287742096305638
6	0.9762910383747445	1.780654555249828
7	0.7066806814468004	1.5037317895731812
8	0.5352113335895832	1.3015606504171857
9	0.42541059270731857	1.163857825147397
>10	2.851418489379151	16.825359049501486
>50	0.32468541363250375	6.880634015080288
>100	0.2506943933628304	15.044760938599563
>500	0.02471037959846019	5.1742261719881615
>1k	0.014325396844694471	8.15617771322923
>5k	0.001082231595808167	2.2162840678346076
>10k+	2.7787027459939015E-4	1.0034019548879471
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.250789254667887E-5	0.0	0.0	8.501578509335775E-6	4.250789254667887E-6
2	5.100947105601465E-5	0.0	0.0	8.501578509335775E-6	1.2752367764003662E-5
3	6.80126280746862E-5	0.0	0.0	1.700315701867155E-5	2.1253946273339435E-5
4	8.926657434802564E-5	0.0	0.0	1.700315701867155E-5	2.5504735528007324E-5
5	1.1052052062136508E-4	0.0	0.0	1.700315701867155E-5	2.975552478267521E-5
6	1.4877762391337604E-4	0.0	0.0	1.700315701867155E-5	2.975552478267521E-5
7	1.8278393795071916E-4	0.0	0.0	1.700315701867155E-5	2.975552478267521E-5
8	2.040378842240586E-4	0.0	0.0	1.700315701867155E-5	2.975552478267521E-5
9	2.677997230440769E-4	0.0	0.0	2.1253946273339435E-5	3.825710329201099E-5
10-11	3.740694544107741E-4	4.250789254667887E-6	2.1253946273339437E-6	2.1253946273339435E-5	4.250789254667887E-5
12-13	5.228470783241501E-4	6.376183882001831E-6	4.250789254667887E-6	2.1253946273339435E-5	4.250789254667887E-5
14-15	6.950040431381995E-4	8.501578509335775E-6	4.250789254667887E-6	2.1253946273339435E-5	5.31348656833486E-5
16-17	8.395308777969078E-4	8.501578509335775E-6	4.250789254667887E-6	3.40063140373431E-5	5.526026031068254E-5
18-19	0.0010244402103749609	8.501578509335775E-6	4.250789254667887E-6	3.40063140373431E-5	5.526026031068254E-5
20-21	0.0013007415119283736	8.501578509335775E-6	4.250789254667887E-6	3.40063140373431E-5	5.738565493801648E-5
22-23	0.001662058598575144	8.501578509335775E-6	4.250789254667887E-6	3.825710329201099E-5	6.376183882001832E-5
24-25	0.0023634388255953454	1.4877762391337605E-5	4.250789254667887E-6	4.250789254667888E-5	6.376183882001832E-5
26-27	0.003283734699230943	1.912855164600549E-5	4.250789254667887E-6	4.675868180134676E-5	7.013802270202014E-5
28-29	0.004578100027277314	2.5504735528007324E-5	4.250789254667887E-6	4.88840764286807E-5	7.226341732935408E-5
30-31	0.006276290334517136	2.5504735528007324E-5	4.250789254667887E-6	5.100947105601465E-5	8.28903904660238E-5
32-33	0.008454819827534427	2.5504735528007324E-5	4.250789254667887E-6	5.31348656833486E-5	9.139196897535958E-5
34-35	0.01109668534931052	2.5504735528007324E-5	4.250789254667887E-6	5.526026031068254E-5	9.77681528573614E-5
36-37	0.013944714149938003	2.975552478267521E-5	4.250789254667887E-6	5.738565493801648E-5	1.2752367764003663E-4
38-39	0.017302837661125635	2.975552478267521E-5	4.250789254667887E-6	6.376183882001832E-5	1.8278393795071916E-4
40-41	0.021415476265016817	2.975552478267521E-5	4.250789254667887E-6	6.80126280746862E-5	2.0191248959672465E-4
42-43	0.02640802824462425	2.975552478267521E-5	4.250789254667887E-6	6.80126280746862E-5	2.1679025198806227E-4
44-45	0.03217634926320857	3.188091941000916E-5	4.250789254667887E-6	6.80126280746862E-5	2.3591880363406777E-4
46-47	0.039030746936360536	3.40063140373431E-5	4.250789254667887E-6	6.80126280746862E-5	2.422949875160696E-4
48-49	0.04658227404727804	3.40063140373431E-5	4.250789254667887E-6	6.80126280746862E-5	2.529219606527393E-4
50-51	0.05557906950478263	3.40063140373431E-5	4.250789254667887E-6	6.80126280746862E-5	2.8480288006274844E-4
52-53	0.06672676432514917	3.40063140373431E-5	6.376183882001831E-6	6.80126280746862E-5	2.954298531994182E-4
54-55	0.08036542164875107	3.40063140373431E-5	8.501578509335775E-6	7.226341732935408E-5	3.1880919410009155E-4
56-57	0.09666932383502977	3.40063140373431E-5	1.2752367764003662E-5	7.226341732935408E-5	3.1880919410009155E-4
58-59	0.11564272167323988	3.40063140373431E-5	1.700315701867155E-5	7.651420658402198E-5	3.1880919410009155E-4
60-61	0.1381719047229797	3.40063140373431E-5	1.700315701867155E-5	7.651420658402198E-5	3.251853779820934E-4
62-63	0.16567663659530824	3.40063140373431E-5	1.700315701867155E-5	7.863960121135592E-5	3.315615618640952E-4
64-65	0.2000740232440808	3.40063140373431E-5	1.700315701867155E-5	8.28903904660238E-5	3.3368695649142917E-4
66-67	0.2418337768819381	3.40063140373431E-5	1.700315701867155E-5	8.501578509335774E-5	3.4006314037343096E-4
68-69	0.29230339770260994	3.40063140373431E-5	1.700315701867155E-5	8.501578509335774E-5	3.613170866467704E-4
70-71	0.3534446249471255	3.613170866467704E-5	1.912855164600549E-5	8.926657434802564E-5	3.8257103292010984E-4
72-73	0.42882387080015116	3.825710329201099E-5	2.1253946273339435E-5	9.77681528573614E-5	3.868218221747777E-4
74-75	0.5231786398860142	3.825710329201099E-5	2.1253946273339435E-5	1.0626973136669718E-4	3.9744879531144746E-4
76-77	0.6375525009667358	3.825710329201099E-5	2.1253946273339435E-5	1.1477130987603296E-4	4.038249791934493E-4
78-79	0.7769103758917678	4.038249791934493E-5	2.1253946273339435E-5	1.1902209913070084E-4	4.1020116307545116E-4
80-81	0.9435349384908669	5.100947105601465E-5	2.1253946273339435E-5	1.1902209913070084E-4	4.2082813621212084E-4
82-83	1.144903327062994	5.100947105601465E-5	2.1253946273339435E-5	1.1902209913070084E-4	4.2082813621212084E-4
84-85	1.3854299862491217	5.100947105601465E-5	2.337934090067338E-5	1.2114749375803478E-4	4.250789254667887E-4
86-87	1.6738247832320647	5.100947105601465E-5	2.5504735528007324E-5	1.3389986152203846E-4	4.335805039761245E-4
88-89	2.0135372334919857	5.100947105601465E-5	2.5504735528007324E-5	1.4877762391337604E-4	4.3783129323079237E-4
90-91	2.404990290559724	5.100947105601465E-5	2.5504735528007324E-5	1.5302841316804395E-4	4.4845826636746215E-4
92-93	2.8568810692503304	5.100947105601465E-5	2.975552478267521E-5	1.5302841316804395E-4	4.5058366099479607E-4
94-95	3.381902426278243	5.526026031068254E-5	2.975552478267521E-5	1.5302841316804395E-4	4.697122126408015E-4
96-97	3.9813891094694283	5.526026031068254E-5	3.40063140373431E-5	1.5302841316804395E-4	4.7608839652280336E-4
98-99	4.655128579361151	5.526026031068254E-5	3.825710329201099E-5	1.5727920242271184E-4	4.8033918577747124E-4
100-101	5.410625604382529	5.526026031068254E-5	3.825710329201099E-5	1.6152999167737972E-4	4.8033918577747124E-4
102-103	6.236364796442668	5.526026031068254E-5	3.825710329201099E-5	1.6365538630471366E-4	4.8033918577747124E-4
104-105	7.14575101945616	5.526026031068254E-5	3.825710329201099E-5	1.700315701867155E-4	4.845899750321392E-4
106-107	8.12658450826211	5.526026031068254E-5	3.825710329201099E-5	1.742823594413834E-4	4.845899750321392E-4
108-109	9.183494372358851	5.526026031068254E-5	3.825710329201099E-5	1.7640775406871733E-4	4.8671536965947315E-4
110-111	10.30249976588778	5.526026031068254E-5	3.825710329201099E-5	1.8278393795071916E-4	4.888407642868071E-4
112-113	11.487496537200805	5.526026031068254E-5	3.825710329201099E-5	1.8703472720538704E-4	4.930915535414749E-4
114-115	12.72807025262398	5.526026031068254E-5	3.825710329201099E-5	1.934109110873889E-4	4.930915535414749E-4
116-117	14.018597117973385	5.526026031068254E-5	3.825710329201099E-5	1.9553630571472283E-4	5.143454998148144E-4
118-119	15.343529871550075	5.526026031068254E-5	3.825710329201099E-5	1.9553630571472283E-4	5.185962890694823E-4
120-121	16.689856847445505	5.526026031068254E-5	3.825710329201099E-5	1.997870949693907E-4	5.185962890694823E-4
122-123	18.054744894621447	5.951104956535042E-5	3.825710329201099E-5	1.997870949693907E-4	5.185962890694823E-4
124-125	19.441095176744206	5.951104956535042E-5	3.825710329201099E-5	2.040378842240586E-4	5.29223262206152E-4
126-127	20.83327329093511	5.951104956535042E-5	3.825710329201099E-5	2.040378842240586E-4	5.738565493801648E-4
128-129	22.247774424896907	5.951104956535042E-5	3.825710329201099E-5	2.040378842240586E-4	5.738565493801648E-4
130-131	23.668851529835674	5.951104956535042E-5	3.825710329201099E-5	2.0828867347872648E-4	5.759819440074987E-4
132-133	25.098136908825204	5.951104956535042E-5	3.825710329201099E-5	2.1253946273339436E-4	5.781073386348326E-4
134-135	26.508565786681025	6.80126280746862E-5	3.825710329201099E-5	2.146648573607283E-4	5.802327332621667E-4
136-137	27.903568550331663	6.80126280746862E-5	3.825710329201099E-5	2.1679025198806224E-4	5.908597063988363E-4
138-139	29.28249695441577	7.013802270202014E-5	4.038249791934493E-5	2.1891564661539618E-4	6.01486679535506E-4
>>END_MODULE
