-----------------------
Start of variable list:
-----------------------
Sample_ID:
IC57-1170
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE111828
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE111828/Scripts/00_Setup_Pipeline
Bowtie2Index_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
STRANDEDNESS:
fr-firststrand
DISTANCE_BT_READ_PAIR:
-86
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Mon Jun 24 22:49:15 EDT 2019
Running on node : scc-kb7
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE111828/Scripts/04_TopHat_Paired_End
Current job ID : 7056813
Current job name : Step_04_IC57-1170
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/7056813.1.linga

Loading required modules...


Here is the Left_List:
SRR6833773_1.fastq.gz


Here is the Right_List:
SRR6833773_2.fastq.gz


List files in the scratch directory:

total 1.1G
drwx------  4 varsha97 waxmanlab 4.0K Jun 24 22:49 .
drwxrwxrwt. 7 root     root      4.0K Jun 24 22:49 ..
drwxr-xr-x  2 varsha97 waxmanlab 4.0K Jun 24 22:49 Bowtie2Index
-rw-r--r--  1 varsha97 waxmanlab  72M Jun 24 22:49 RefSeq_GeneBody.gtf
-rw-r--r--  1 varsha97 waxmanlab 501M Jun 24 22:49 SRR6833773_1.fastq.gz
-rw-r--r--  1 varsha97 waxmanlab 525M Jun 24 22:49 SRR6833773_2.fastq.gz
drwxr-xr-x  2 varsha97 waxmanlab 4.0K Jun 24 22:49 tophat2

Starting to run my commands


Starting tophat

tophat --no-coverage-search -o /scratch/7056813.1.linga/tophat2 -G RefSeq_GeneBody.gtf -p 16 --library-type fr-firststrand -r -86 --mate-std-dev 150 Bowtie2Index/genome SRR6833773_1.fastq.gz SRR6833773_2.fastq.gz

Ending tophat


Starting samtools commands


Ending samtools commands


Starting spliced_read_counts


Ending spliced_read_counts


List files in scratch

total 1.1G
drwx------  4 varsha97 waxmanlab  20K Jun 25 00:21 .
drwxrwxrwt. 8 root     root      4.0K Jun 25 00:07 ..
drwxr-xr-x  2 varsha97 waxmanlab 4.0K Jun 24 22:49 Bowtie2Index
-rw-r--r--  1 varsha97 waxmanlab  72M Jun 24 22:49 RefSeq_GeneBody.gtf
-rw-r--r--  1 varsha97 waxmanlab 501M Jun 24 22:49 SRR6833773_1.fastq.gz
-rw-r--r--  1 varsha97 waxmanlab 525M Jun 24 22:49 SRR6833773_2.fastq.gz
-rw-r--r--  1 varsha97 waxmanlab    9 Jun 25 00:21 numunique_hits.txt
drwxr-xr-x  3 varsha97 waxmanlab 4.0K Jun 25 00:40 tophat2
==========================================================
Finished on : Tue Jun 25 00:41:00 EDT 2019
1 hours, 51 minutes and 45 seconds elapsed.
==========================================================