anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 anaconda3/5.2.0(77):ERROR:150: Module 'anaconda3/5.2.0' conflicts with the currently loaded module(s) 'anaconda2/5.2.0' anaconda3/5.2.0(77):ERROR:102: Tcl command execution failed: conflict python2 python3 python2-intel python3-intel anaconda2 anaconda3 [2019-06-24 21:19:30] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-06-24 21:19:30] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-06-24 21:19:30] Checking for Bowtie index files (genome).. [2019-06-24 21:19:30] Checking for reference FASTA file [2019-06-24 21:19:30] Generating SAM header for Bowtie2Index/genome [2019-06-24 21:19:35] Reading known junctions from GTF file [2019-06-24 21:19:38] Preparing reads left reads: min. length=32, max. length=36, 22773669 kept reads (33842 discarded) right reads: min. length=32, max. length=36, 22640595 kept reads (166916 discarded) [2019-06-24 21:29:21] Building transcriptome data files /scratch/7056803.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-06-24 21:29:40] Building Bowtie index from RefSeq_GeneBody.fa [2019-06-24 21:36:45] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-24 21:47:34] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-24 21:58:12] Resuming TopHat pipeline with unmapped reads Warning: you have only one segment per read. If the read length is greater than or equal to 45bp, we strongly recommend that you decrease --segment-length to about half the read length because TopHat will work better with multiple segments [2019-06-24 21:58:12] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-24 22:00:36] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-24 22:04:09] Searching for junctions via segment mapping [2019-06-24 22:06:28] Retrieving sequences for splices [2019-06-24 22:08:37] Indexing splices Building a SMALL index [2019-06-24 22:08:50] Mapping left_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1) [2019-06-24 22:08:53] Joining segment hits [2019-06-24 22:14:58] Mapping right_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1) [2019-06-24 22:15:01] Joining segment hits [2019-06-24 22:21:25] Reporting output tracks ----------------------------------------------- [2019-06-24 23:15:28] A summary of the alignment counts can be found in /scratch/7056803.1.linga/tophat2/align_summary.txt [2019-06-24 23:15:28] Run complete: 01:55:58 elapsed [samopen] SAM header is present: 22 sequences. [bam_sort_core] merging from 13 files...