[2019-06-24 21:23:18] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-06-24 21:23:18] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-06-24 21:23:18] Checking for Bowtie index files (genome).. [2019-06-24 21:23:18] Checking for reference FASTA file [2019-06-24 21:23:18] Generating SAM header for Bowtie2Index/genome [2019-06-24 21:23:21] Reading known junctions from GTF file [2019-06-24 21:23:24] Preparing reads left reads: min. length=32, max. length=36, 13355729 kept reads (77135 discarded) right reads: min. length=32, max. length=36, 12996020 kept reads (436844 discarded) [2019-06-24 21:28:35] Building transcriptome data files /scratch/7056804.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-06-24 21:28:50] Building Bowtie index from RefSeq_GeneBody.fa [2019-06-24 21:34:45] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-24 21:38:46] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2