[2019-06-24 19:37:24] Beginning TopHat run (v2.1.1)
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[2019-06-24 19:37:24] Checking for Bowtie
		  Bowtie version:	 2.3.4.1
[2019-06-24 19:37:25] Checking for Bowtie index files (genome)..
[2019-06-24 19:37:25] Checking for reference FASTA file
[2019-06-24 19:37:25] Generating SAM header for Bowtie2Index/genome
[2019-06-24 19:37:27] Reading known junctions from GTF file
[2019-06-24 19:37:29] Preparing reads
	 left reads: min. length=32, max. length=36, 20294722 kept reads (25089 discarded)
	right reads: min. length=32, max. length=36, 20204975 kept reads (114836 discarded)
[2019-06-24 19:42:24] Building transcriptome data files /scratch/7056796.1.c/tophat2/tmp/RefSeq_GeneBody
[2019-06-24 19:42:33] Building Bowtie index from RefSeq_GeneBody.fa
[2019-06-24 19:47:22] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 
[2019-06-24 19:51:50] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 
[2019-06-24 19:56:19] Resuming TopHat pipeline with unmapped reads
Warning: you have only one segment per read.
	If the read length is greater than or equal to 45bp,
	we strongly recommend that you decrease --segment-length to about half the read length because TopHat will work better with multiple segments
[2019-06-24 19:56:20] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 
[2019-06-24 19:57:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 
[2019-06-24 19:59:01] Searching for junctions via segment mapping
[2019-06-24 20:00:12] Retrieving sequences for splices
[2019-06-24 20:01:25] Indexing splices
[2019-06-24 20:01:33] Mapping left_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1)
open: No such file or directory
Error: bam2fastx failed to open BAM file /scratch/7056796.1.c/tophat2/tmp/left_kept_reads.m2g_um_unmapped.bam
[2019-06-24 20:01:35] Joining segment hits
[2019-06-24 20:04:11] Mapping right_kept_reads.m2g_um_unmapped to genome segment_juncs with Bowtie2 (1/1)
open: No such file or directory
Error: bam2fastx failed to open BAM file /scratch/7056796.1.c/tophat2/tmp/right_kept_reads.m2g_um_unmapped.bam
[2019-06-24 20:04:13] Joining segment hits
[2019-06-24 20:06:52] Reporting output tracks
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[2019-06-24 20:20:16] A summary of the alignment counts can be found in /scratch/7056796.1.c/tophat2/align_summary.txt
[2019-06-24 20:20:16] Run complete: 00:42:51 elapsed