FastQCFastQC Report
Mon 17 Jun 2019
SRR5865516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5865516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58757698
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2035230.34637674198876883Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090350.1855671745343053No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1014060.17258334388797872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)