##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5865515_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68440176
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.791180928582065	33.0	33.0	33.0	33.0	33.0
2	31.86651266063372	33.0	33.0	33.0	33.0	33.0
3	31.90795142023013	33.0	33.0	33.0	33.0	33.0
4	31.943588119937036	33.0	33.0	33.0	33.0	33.0
5	31.971128347186017	33.0	33.0	33.0	33.0	33.0
6	35.557865952887084	37.0	37.0	37.0	33.0	37.0
7	35.511665034292136	37.0	37.0	37.0	33.0	37.0
8	35.515829795645175	37.0	37.0	37.0	33.0	37.0
9	35.52716021653714	37.0	37.0	37.0	33.0	37.0
10	35.52324836219007	37.0	37.0	37.0	33.0	37.0
11	35.53283987755964	37.0	37.0	37.0	33.0	37.0
12	35.50751742660627	37.0	37.0	37.0	33.0	37.0
13	35.51538957176265	37.0	37.0	37.0	33.0	37.0
14	35.51482258023416	37.0	37.0	37.0	33.0	37.0
15	35.51688953868266	37.0	37.0	37.0	33.0	37.0
16	35.51500149561275	37.0	37.0	37.0	33.0	37.0
17	35.521102809554435	37.0	37.0	37.0	33.0	37.0
18	35.51742812584234	37.0	37.0	37.0	33.0	37.0
19	35.513587077859064	37.0	37.0	37.0	33.0	37.0
20	35.51761029954102	37.0	37.0	37.0	33.0	37.0
21	35.51710270295038	37.0	37.0	37.0	33.0	37.0
22	35.50579045851665	37.0	37.0	37.0	33.0	37.0
23	35.49739077234401	37.0	37.0	37.0	33.0	37.0
24	35.490731555102954	37.0	37.0	37.0	33.0	37.0
25	35.490079584248875	37.0	37.0	37.0	33.0	37.0
26	35.442867651304695	37.0	37.0	37.0	33.0	37.0
27	35.44262799674858	37.0	37.0	37.0	33.0	37.0
28	35.45598857022226	37.0	37.0	37.0	33.0	37.0
29	35.41879931752367	37.0	37.0	37.0	33.0	37.0
30	35.441617026233246	37.0	37.0	37.0	33.0	37.0
31	35.44415221842796	37.0	37.0	37.0	33.0	37.0
32	35.44159337053721	37.0	37.0	37.0	33.0	37.0
33	35.43869862345181	37.0	37.0	37.0	33.0	37.0
34	35.43714316281127	37.0	37.0	37.0	33.0	37.0
35	35.37236455967033	37.0	37.0	37.0	33.0	37.0
36	35.3928846559366	37.0	37.0	37.0	33.0	37.0
37	35.38645704242491	37.0	37.0	37.0	33.0	37.0
38	35.38903000483225	37.0	37.0	37.0	33.0	37.0
39	35.374156679550325	37.0	37.0	37.0	33.0	37.0
40	35.353249310755714	37.0	37.0	37.0	33.0	37.0
41	35.340926008138844	37.0	37.0	37.0	33.0	37.0
42	35.32667996061261	37.0	37.0	37.0	33.0	37.0
43	35.30443580390559	37.0	37.0	37.0	33.0	37.0
44	35.27287476583929	37.0	37.0	37.0	33.0	37.0
45	35.239389828570864	37.0	37.0	37.0	33.0	37.0
46	35.15582833977516	37.0	37.0	37.0	33.0	37.0
47	35.12316613563355	37.0	37.0	37.0	33.0	37.0
48	35.02397338954827	37.0	37.0	37.0	33.0	37.0
49	34.86498994391832	37.0	37.0	37.0	33.0	37.0
50	34.30329403302528	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	416112.0
3	55860.0
4	34228.0
5	40979.0
6	40525.0
7	39585.0
8	37055.0
9	35786.0
10	36576.0
11	36308.0
12	37978.0
13	38911.0
14	41345.0
15	43316.0
16	47463.0
17	53053.0
18	59210.0
19	68521.0
20	79465.0
21	97608.0
22	128587.0
23	184640.0
24	248066.0
25	319808.0
26	404528.0
27	499305.0
28	613613.0
29	753956.0
30	927055.0
31	1159476.0
32	1490811.0
33	2031795.0
34	3101109.0
35	6992915.0
36	4.8244628E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.605399459844282	30.805622752759497	27.839415051535426	21.749562735860795
2	22.159592422778243	30.46603553667349	31.096968767084764	16.277403273463502
3	20.844983536459615	28.89392755093032	31.19656739354032	19.06452151906974
4	20.749468920282393	28.459660368554925	30.97887479157525	19.81199591958743
5	23.291783931430075	28.89721699571115	28.981175492492227	18.829823580366543
6	21.299046938851138	28.305276030630015	30.36566454655589	20.03001248396295
7	22.271172067122567	28.63384308481423	29.547830761141324	19.54715408692188
8	20.462370134772733	28.268628712754325	30.947880395862686	20.321120756610256
9	21.54709065152662	27.856274541447934	30.48488169615583	20.111753110869614
10	20.997034931016486	29.39191368019144	29.849680360885134	19.76137102790694
11	22.659095474604754	28.31861695509274	29.101194962247913	19.921092608054593
12	21.114384509221264	29.374784085643178	29.12370752746911	20.387123877666443
13	22.363285137648216	28.31150229959284	29.12934622021225	20.195866342546694
14	22.047162106809	28.339154034527926	29.235464194976096	20.378219663686984
15	21.801305073792708	28.40963146251744	29.048044798649997	20.741018665039856
16	21.97371267219705	28.263744370478648	29.15960593010053	20.602937027223778
17	21.695990064551104	28.405487169574478	29.40962162466571	20.488901141208707
18	21.861264739818072	28.352721492453835	29.07323061453388	20.712783153194216
19	21.57044574717259	28.452965203869855	29.387890783488007	20.58869826546954
20	21.409964210368777	28.767139172218204	29.11385264303411	20.709043974378908
21	21.600270305176352	28.54788611486661	29.07746264705483	20.774380932902204
22	21.584840489890762	28.477184084890645	29.20396016615991	20.734015259058687
23	21.73504482990369	28.540747982642984	29.07846142071431	20.645745766739015
24	21.333813344432528	28.86128621704316	28.947382485292657	20.857517953231657
25	21.3262940885833	29.01027320542286	28.890526350099304	20.772906355894534
26	21.451746615871436	28.994068595466754	28.849301546064993	20.704883242596814
27	21.55284633766852	28.58921585520825	28.880641589691937	20.977296217431295
28	21.342896899001886	28.784196452573873	29.073119261919906	20.799787386504338
29	21.566687172119416	28.809278100139856	28.958817222407195	20.665217505333533
30	21.38880991960019	28.68087137678308	28.87254381209222	21.057774891524506
31	21.66394411461408	28.72395530433157	28.6912826296044	20.920817951449948
32	21.535004966410302	28.75028411024155	28.740132948875914	20.97457797447223
33	21.505737820149285	28.96629167506159	28.40515092089494	21.122819583894188
34	21.777396469594862	28.700527762837314	28.457789195868045	21.064286571699782
35	21.452633220237484	28.872172982709394	28.47549315782481	21.199700639228308
36	21.55609497404451	28.61071896166202	28.74445192466813	21.088734139625345
37	21.475692456483035	28.697590648623667	28.575287723040667	21.25142917185264
38	21.397493986808065	28.790068563336046	28.822425318599283	20.99001213125661
39	21.388564756691594	28.70700171538053	28.557301024485586	21.347132503442285
40	21.811954833501122	28.6440938458676	28.41693990216383	21.127011418467447
41	21.6482881564715	28.64476181371401	28.488926162960986	21.218023866853507
42	21.542018302420555	28.560130021498004	28.60734841048707	21.29050326559437
43	21.809931768294135	28.69845686866449	28.373336360157957	21.118275002883415
44	21.857806845779148	28.842442543505587	28.241225429583555	21.05852518113171
45	21.586558291208803	28.69614915012737	28.624692707710842	21.092599850952986
46	21.368850228747885	28.71893729125354	28.526372016051493	21.38584046394708
47	21.63784191013055	28.683385731462085	28.58027538920254	21.098496969204817
48	21.470230787239434	28.739893493989115	28.404778791692962	21.385096927078486
49	21.411976597648508	28.89274387044774	28.386106077229368	21.30917345467439
50	21.614908744595496	28.730444739509824	28.443918721412082	21.210727794482594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190285.0
1	107060.0
2	23835.0
3	21681.0
4	19527.0
5	22045.5
6	24564.0
7	25195.5
8	25827.0
9	26096.0
10	26365.0
11	32078.0
12	37791.0
13	63789.5
14	89788.0
15	193507.5
16	297227.0
17	508778.5
18	720330.0
19	779647.0
20	838964.0
21	855243.0
22	871522.0
23	1096671.0
24	1321820.0
25	1817758.5
26	2313697.0
27	2730201.0
28	3146705.0
29	3154199.5
30	3161694.0
31	3096207.0
32	3030720.0
33	3164668.5
34	3298617.0
35	3609791.0
36	3920965.0
37	4218950.5
38	4516936.0
39	4596768.0
40	4676600.0
41	4664856.5
42	4653113.0
43	4491608.0
44	4330103.0
45	4229253.0
46	4128403.0
47	3856289.5
48	3584176.0
49	3583728.0
50	3583280.0
51	3350109.0
52	3116938.0
53	2878663.0
54	2640388.0
55	2456911.5
56	2273435.0
57	2144971.5
58	2016508.0
59	1756865.0
60	1497222.0
61	1284397.0
62	1071572.0
63	922715.0
64	773858.0
65	641208.5
66	508559.0
67	459034.0
68	409509.0
69	371852.0
70	334195.0
71	291526.0
72	248857.0
73	230491.5
74	212126.0
75	165151.5
76	118177.0
77	103512.5
78	88848.0
79	89484.5
80	90121.0
81	78983.0
82	67845.0
83	60866.5
84	53888.0
85	43272.0
86	32656.0
87	35957.5
88	39259.0
89	25811.5
90	12364.0
91	6360.5
92	357.0
93	274.5
94	192.0
95	165.0
96	138.0
97	136.0
98	134.0
99	155.0
100	176.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23953620458252475
2	0.2461288235144223
3	0.2513918140713139
4	0.2550110332854784
5	0.25642102381501763
6	0.24632607607554957
7	0.24119750948624094
8	0.2465291731570065
9	0.24609813978269138
10	0.2477915895482209
11	0.24323578595122258
12	0.24339212687004194
13	0.2411536755837682
14	0.2426089611458626
15	0.2399029482332132
16	0.22429661782284138
17	0.21410377436785083
18	0.2130751387898243
19	0.2059360572071001
20	0.20325634463593428
21	0.20613184863814493
22	0.21608652788970031
23	0.2122276833420183
24	0.21118151420300263
25	0.21348133295273816
26	0.21883929696498733
27	0.2212487004709047
28	0.22063356470620413
29	0.22066424843793503
30	0.21493369625466774
31	0.22018499777089995
32	0.22270983055332877
33	0.23349297056161866
34	0.2363816247345711
35	0.2377156364998243
36	0.24591111513214112
37	0.2405341564288204
38	0.24393858952086855
39	0.2444149179277388
40	0.24350171162622375
41	0.24302246095918864
42	0.2424423923164663
43	0.236412308466302
44	0.2469295227995907
45	0.24315542379668925
46	0.24205811510478875
47	0.24642543292115437
48	0.2415890923483306
49	0.24680971013283193
50	0.243709192097928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6.8440176E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.42814644453702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02825252321558	30.753374076625178
2	12.358661519738169	9.498409094779255
3	3.3397275286616304	3.8501861566878257
4	1.4270822237518057	2.193604987309197
5	0.7551858550288912	1.4510196314946566
6	0.43575793624523396	1.0047221873040644
7	0.2983320123390876	0.8025042381481918
8	0.20958734531198514	0.6443242558856571
9	0.148266682151319	0.5127852397100858
>10	0.8389471571841738	5.847325913068273
>50	0.06465100303113543	1.7556314874984276
>100	0.06611485669724596	5.5345393672907255
>500	0.010837137340871621	2.865370227568884
>1k	0.01261404227470495	12.105757295298723
>5k	0.004210814524481711	11.268339430566218
>10k+	0.001771362504040542	9.91210641076492
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	133252	0.19469850574317635	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	120184	0.1756044578260582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5341865865453063E-4	0.0	0.0	4.383390247272304E-6	0.0
2	2.0455821153937418E-4	2.9222601648482026E-6	0.0	1.168904065939281E-5	0.0
3	2.5131437417694544E-4	4.383390247272304E-6	0.0	1.7533560989089217E-5	0.0
4	2.6592567500118643E-4	5.844520329696405E-6	0.0	2.6300341483633822E-5	0.0
5	2.9807053681451666E-4	8.766780494544609E-6	0.0	3.068373173090613E-5	0.0
6	3.345987888751192E-4	1.022791057696871E-5	0.0	3.945051222545074E-5	2.9222601648482026E-6
7	3.915828620896592E-4	1.022791057696871E-5	0.0	4.821729271999534E-5	2.9222601648482026E-6
8	4.251888539854135E-4	1.168904065939281E-5	0.0	5.2600682967267645E-5	2.9222601648482026E-6
9	4.573337157987437E-4	1.3150170741816911E-5	0.0	5.406181304969175E-5	4.383390247272304E-6
10	5.099343987660114E-4	1.3150170741816911E-5	0.0	6.136746346181226E-5	4.383390247272304E-6
11	5.654573418981272E-4	1.3150170741816911E-5	0.0	6.428972362666046E-5	4.383390247272304E-6
12	6.881922688217518E-4	1.6072430906665115E-5	0.0	7.013424395635687E-5	4.383390247272304E-6
13	7.65632163190229E-4	1.6072430906665115E-5	0.0	7.451763420362917E-5	4.383390247272304E-6
14	8.313830168993136E-4	1.6072430906665115E-5	0.0	8.620667486302197E-5	4.383390247272304E-6
15	0.0013939180986325926	2.045582115393742E-5	0.0	9.205119519271838E-5	4.383390247272304E-6
16	0.0014772025133307665	2.337808131878562E-5	0.0	9.935684560483889E-5	4.383390247272304E-6
17	0.001586787269512574	2.4839211401209723E-5	0.0	1.0081797568726299E-4	4.383390247272304E-6
18	0.0017139055866834708	2.6300341483633822E-5	0.0	1.0520136593453529E-4	4.383390247272304E-6
19	0.0018994691071513316	2.6300341483633822E-5	0.0	1.15429276511504E-4	4.383390247272304E-6
20	0.007562809306627148	2.7761471566057925E-5	0.0	1.1981266675877631E-4	5.844520329696405E-6
21	0.007733761526270768	2.9222601648482027E-5	0.0	1.2565718708847272E-4	5.844520329696405E-6
22	0.007895946965419844	3.068373173090613E-5	0.0	1.3150170741816911E-4	5.844520329696405E-6
23	0.00805813240456892	3.214486181333023E-5	0.0	1.373462277478655E-4	5.844520329696405E-6
24	0.008214473323388297	3.214486181333023E-5	0.0	1.4465187815998603E-4	7.305650412120507E-6
25	0.008388347803196765	3.5067121978178434E-5	0.0	1.4757413832483423E-4	7.305650412120507E-6
26	0.008603133925313108	3.5067121978178434E-5	0.0	1.5049639848968243E-4	7.305650412120507E-6
27	0.008892437681633081	3.5067121978178434E-5	0.0	1.5487978873695473E-4	7.305650412120507E-6
28	0.00951926248699302	3.798938214302663E-5	0.0	1.6656882939634755E-4	1.022791057696871E-5
29	0.009810027373395416	3.798938214302663E-5	0.0	1.7095221964361985E-4	1.022791057696871E-5
30	0.010664788471613515	3.945051222545074E-5	0.0	1.7533560989089214E-4	1.168904065939281E-5
31	0.03397127441636036	4.091164230787484E-5	0.0	1.8556352046786087E-4	1.3150170741816911E-5
32	0.03445344734356031	4.091164230787484E-5	0.0	1.9433030096240546E-4	1.3150170741816911E-5
33	0.034690150416913014	4.091164230787484E-5	0.0	1.9871369120967778E-4	1.3150170741816911E-5
34	0.03493123688051299	4.091164230787484E-5	0.0	2.1040273186907058E-4	1.4611300824241014E-5
35	0.03516647882378327	4.091164230787484E-5	0.0	2.220917725284634E-4	1.6072430906665115E-5
36	0.03543240449878446	4.237277239029894E-5	0.0	2.3524194327028032E-4	1.7533560989089217E-5
37	0.035723169385186854	4.237277239029894E-5	0.0	2.4254759368240081E-4	1.7533560989089217E-5
38	0.03609721868628742	4.237277239029894E-5	0.0	2.4546985384724904E-4	1.8994691071513316E-5
39	0.03644204538573951	4.3833902472723036E-5	0.0	2.5131437417694544E-4	2.045582115393742E-5
>>END_MODULE
