##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5865514_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57237978
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751655867368342	33.0	33.0	33.0	33.0	33.0
2	31.82614619964388	33.0	33.0	33.0	33.0	33.0
3	31.874593508526804	33.0	33.0	33.0	33.0	33.0
4	31.91402844454079	33.0	33.0	33.0	33.0	33.0
5	31.93725597714161	33.0	33.0	33.0	33.0	33.0
6	35.50996025750595	37.0	37.0	37.0	33.0	37.0
7	35.45389845881698	37.0	37.0	37.0	33.0	37.0
8	35.452656241630336	37.0	37.0	37.0	33.0	37.0
9	35.45345633977497	37.0	37.0	37.0	33.0	37.0
10	35.44223691829226	37.0	37.0	37.0	33.0	37.0
11	35.45509512932131	37.0	37.0	37.0	33.0	37.0
12	35.42645386599785	37.0	37.0	37.0	33.0	37.0
13	35.44201755345026	37.0	37.0	37.0	33.0	37.0
14	35.445679055958266	37.0	37.0	37.0	33.0	37.0
15	35.4444434427785	37.0	37.0	37.0	33.0	37.0
16	35.446510287278144	37.0	37.0	37.0	33.0	37.0
17	35.45495957247127	37.0	37.0	37.0	33.0	37.0
18	35.451552201931385	37.0	37.0	37.0	33.0	37.0
19	35.448669780054075	37.0	37.0	37.0	33.0	37.0
20	35.45041425118127	37.0	37.0	37.0	33.0	37.0
21	35.45257748273358	37.0	37.0	37.0	33.0	37.0
22	35.44371971001491	37.0	37.0	37.0	33.0	37.0
23	35.43745374792939	37.0	37.0	37.0	33.0	37.0
24	35.42041946345484	37.0	37.0	37.0	33.0	37.0
25	35.42692390356626	37.0	37.0	37.0	33.0	37.0
26	35.382295160741	37.0	37.0	37.0	33.0	37.0
27	35.38308888200069	37.0	37.0	37.0	33.0	37.0
28	35.391312547763306	37.0	37.0	37.0	33.0	37.0
29	35.35904479365082	37.0	37.0	37.0	33.0	37.0
30	35.38203079081515	37.0	37.0	37.0	33.0	37.0
31	35.38346197344707	37.0	37.0	37.0	33.0	37.0
32	35.38309172976027	37.0	37.0	37.0	33.0	37.0
33	35.37825067824723	37.0	37.0	37.0	33.0	37.0
34	35.3781467088163	37.0	37.0	37.0	33.0	37.0
35	35.31058431169598	37.0	37.0	37.0	33.0	37.0
36	35.33232328717133	37.0	37.0	37.0	33.0	37.0
37	35.323843637523325	37.0	37.0	37.0	33.0	37.0
38	35.329180635975646	37.0	37.0	37.0	33.0	37.0
39	35.311292984528556	37.0	37.0	37.0	33.0	37.0
40	35.29210161826471	37.0	37.0	37.0	33.0	37.0
41	35.27711261917743	37.0	37.0	37.0	33.0	37.0
42	35.26183805794118	37.0	37.0	37.0	33.0	37.0
43	35.23661251625625	37.0	37.0	37.0	33.0	37.0
44	35.20511402411874	37.0	37.0	37.0	33.0	37.0
45	35.17028253513777	37.0	37.0	37.0	33.0	37.0
46	35.08106187818165	37.0	37.0	37.0	33.0	37.0
47	35.05020042811435	37.0	37.0	37.0	33.0	37.0
48	34.942075539425936	37.0	37.0	37.0	33.0	37.0
49	34.782476767435774	37.0	37.0	37.0	33.0	37.0
50	34.202731829555546	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	364033.0
3	50596.0
4	30407.0
5	36278.0
6	36209.0
7	35205.0
8	31999.0
9	30973.0
10	31132.0
11	31507.0
12	32595.0
13	32983.0
14	35606.0
15	37919.0
16	40996.0
17	45321.0
18	51520.0
19	59464.0
20	69324.0
21	84961.0
22	112927.0
23	161830.0
24	217645.0
25	279945.0
26	351521.0
27	435540.0
28	536001.0
29	653997.0
30	803654.0
31	1005007.0
32	1294840.0
33	1766154.0
34	2698946.0
35	6044134.0
36	3.9706809E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.210411474242267	29.33899762808737	27.80826891602554	22.642321981644816
2	22.673100529124252	29.848616007174574	30.580459046519227	16.897824417181955
3	21.319135386662154	27.837985006895455	31.015399090437473	19.82748051600492
4	21.080791673320252	27.196612940394736	30.78498864761655	20.937606738668464
5	24.38910309527918	27.61639750739907	28.713822424752273	19.280676972569484
6	21.84251344496202	27.08317246373702	30.76999274499648	20.30432134630448
7	22.872208335472795	27.951320309856136	29.386280201344984	19.79019115332608
8	20.995593438939224	27.59331382159112	30.889722099621693	20.521370639847966
9	22.505174031200617	26.982964139303423	30.155489594037764	20.356372235458192
10	21.515244864262073	28.997971206825884	29.43565068384528	20.051133245066758
11	23.90125151541007	27.32955113038768	28.434942340511892	20.334255013690356
12	21.62483542465317	28.742303019128585	28.474851114674525	21.158010441543716
13	23.483154737207624	27.346781182262898	28.513951246903837	20.656112833625645
14	22.822988317975636	27.50674543567186	28.808810184253673	20.86145606209883
15	22.492028636996704	27.68610192490425	28.22662384544544	21.595245592653608
16	23.064510480755658	27.4869036517091	28.31728514862372	21.13130071891152
17	22.353412037417996	27.597628722847357	29.01149397113639	21.03746526859826
18	22.900359689652667	27.494281723250374	28.34715649611908	21.258202090977875
19	22.17474280989709	27.640140818761232	29.067401854500467	21.11771451684121
20	21.944829228641783	28.312261934472264	28.463126049841144	21.27978278704481
21	22.499953432363906	27.745484269802663	28.416905550470073	21.33765674736336
22	22.43623242747409	27.70880258416471	28.549565493747934	21.305399494613265
23	22.57686904770096	27.824207139363246	28.439924254285664	21.158999558650134
24	21.829772426454742	28.44951185869685	28.286923561125604	21.4337921537228
25	21.83115818319303	28.63463202720445	28.223508429082482	21.31070136052004
26	21.92814565217201	28.619631037142284	28.166160224742903	21.2860630859428
27	22.41741938181419	27.890847788295993	28.153711823178817	21.538021006711
28	21.822916888266427	28.486463080109655	28.350403234087985	21.340216797535934
29	22.4336265320392	28.08301708160301	28.276238417356613	21.207117969001175
30	21.882322048661187	28.010485526908603	28.521054786272316	21.586137638157897
31	22.513734778591125	28.025409176245958	27.996520945893007	21.464335099269917
32	22.02875363614846	28.008900485296053	28.42103145433226	21.54131442422322
33	22.020359310923283	28.56031662738932	27.744719097314384	21.67460496437301
34	22.663533345800634	27.9511699142497	27.794659143044775	21.590637596904894
35	22.018018287483752	28.438704735160925	27.787322270835467	21.755954706519855
36	22.147830114605387	27.855211105228328	28.39125915765759	21.6056996225087
37	22.02666251407882	27.939271975275293	27.898008785791507	22.136056724854384
38	21.995494593250957	28.02681282516401	28.47864772438325	21.49904485720179
39	21.97355256168731	27.92922270829236	27.873978956445804	22.223245773574522
40	22.71517662313308	27.91852081700898	27.73513642087697	21.63116613898097
41	22.571518738391067	27.897525097013297	27.775913031813076	21.75504313278256
42	22.0829132924386	27.795997768219248	28.313372448281665	21.807716491060486
43	22.640978725398373	27.952230370375197	27.751672260305853	21.65511864392058
44	22.795034800141316	28.037405274338294	27.57544724299137	21.59211268252902
45	22.14426226545332	27.918411711443525	28.30740647843703	21.629919544666123
46	21.891694034321233	27.99632166673771	27.812415511345524	22.299568787595536
47	22.53052791333168	27.906560896207022	27.898888154793433	21.664023035667864
48	21.987496399799973	27.944823517432987	27.75388463465277	22.313795448114266
49	21.99780377981054	28.116338372029894	27.73134832857836	22.15450951958121
50	22.435828876115636	27.9533053312058	27.842353802538643	21.768511990139917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157306.0
1	88078.0
2	18850.0
3	17242.0
4	15634.0
5	17742.5
6	19851.0
7	20688.5
8	21526.0
9	20707.5
10	19889.0
11	23498.0
12	27107.0
13	46257.0
14	65407.0
15	147169.5
16	228932.0
17	382997.0
18	537062.0
19	583541.0
20	630020.0
21	650614.5
22	671209.0
23	838454.5
24	1005700.0
25	1379591.0
26	1753482.0
27	2088036.0
28	2422590.0
29	2447763.0
30	2472936.0
31	2429718.5
32	2386501.0
33	2473200.5
34	2559900.0
35	2810012.0
36	3060124.0
37	3302260.0
38	3544396.0
39	3637619.0
40	3730842.0
41	3743098.0
42	3755354.0
43	3658382.5
44	3561411.0
45	3481113.5
46	3400816.0
47	3179205.0
48	2957594.0
49	3002620.5
50	3047647.0
51	2851254.5
52	2654862.0
53	2473104.0
54	2291346.0
55	2156134.0
56	2020922.0
57	2017971.0
58	2015020.0
59	1717925.0
60	1420830.0
61	1232714.0
62	1044598.0
63	917717.0
64	790836.0
65	670451.0
66	550066.0
67	519113.5
68	488161.0
69	462223.0
70	436285.0
71	389036.0
72	341787.0
73	319055.0
74	296323.0
75	234136.0
76	171949.0
77	152378.0
78	132807.0
79	138444.5
80	144082.0
81	124026.5
82	103971.0
83	95370.0
84	86769.0
85	72139.0
86	57509.0
87	71812.5
88	86116.0
89	58208.5
90	30301.0
91	15429.5
92	558.0
93	401.0
94	244.0
95	227.5
96	211.0
97	186.5
98	162.0
99	169.5
100	177.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2375328492561355
2	0.24401455970369884
3	0.24878237312995227
4	0.2527709836290863
5	0.25389611072564444
6	0.243637187882493
7	0.23827186907266362
8	0.24408793755782218
9	0.24348519089895174
10	0.24544368076733947
11	0.2409623903905201
12	0.241084686814059
13	0.23883093843741302
14	0.24025132404222943
15	0.2371869250866968
16	0.22124820691604444
17	0.21153437670352365
18	0.2108442754564111
19	0.2037318648817399
20	0.2010029075450569
21	0.20399392864646615
22	0.21334436377189983
23	0.20969818325867487
24	0.208735535696247
25	0.21113254559761005
26	0.21633887905683882
27	0.2187044413064347
28	0.2181680841346282
29	0.21789728491107774
30	0.21233279764005641
31	0.2179549389393175
32	0.22019645767360965
33	0.2308187756038482
34	0.2339722762393878
35	0.23526337705360592
36	0.2431584847389263
37	0.2380167936749967
38	0.24134150930349077
39	0.24163676781174906
40	0.2405745360187252
41	0.24032644898811764
42	0.2400276962963297
43	0.2338132908887872
44	0.24447753902138192
45	0.2406164662210814
46	0.23955423442805754
47	0.2441420974025323
48	0.2393236183150984
49	0.24437970188255076
50	0.24156863123292022
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5.7237978E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.81056422508706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72180299660722	24.844750804595712
2	14.317952255763094	9.395601841188903
3	4.435990671648378	4.366420705020186
4	1.8748986668672494	2.46065932499112
5	0.9906645970859336	1.625213219410401
6	0.5897504153029685	1.161002632684189
7	0.38691106611181847	0.8886339268841107
8	0.2856525570885703	0.7497937256331907
9	0.18977126196284858	0.5603851960837077
>10	0.9800512945351362	5.756844437428004
>50	0.0814054647246101	1.8721882972633115
>100	0.09691197286480444	7.0110427437349925
>500	0.018816767929135125	4.3161533872070095
>1k	0.02313316804567166	17.550166961118936
>5k	0.005040175889492789	11.633943624682749
>10k+	0.001246667574244548	5.8071991720734815
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	300086	0.5242777793443367	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	99648	0.17409420018296246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2146488473090365E-4	0.0	0.0	3.4941835296837354E-6	0.0
2	3.6863636238163407E-4	1.7470917648418677E-6	0.0	8.73545882420934E-6	0.0
3	4.734618682721462E-4	1.7470917648418677E-6	0.0	1.3976734118734942E-5	0.0
4	5.293688047470859E-4	3.4941835296837354E-6	0.0	1.572382588357681E-5	0.0
5	5.695519153384489E-4	5.241275294525603E-6	0.0	2.0965101178102412E-5	0.0
6	6.132292094594956E-4	1.0482550589051206E-5	0.0	2.970056000231175E-5	0.0
7	6.901012471125377E-4	1.572382588357681E-5	0.0	3.144765176715362E-5	0.0
8	7.250430824093751E-4	1.572382588357681E-5	0.0	3.144765176715362E-5	0.0
9	8.141447624163104E-4	1.572382588357681E-5	0.0	3.494183529683736E-5	1.7470917648418677E-6
10	8.9800516712872E-4	1.9218009413260544E-5	0.0	4.1930202356204825E-5	1.7470917648418677E-6
11	9.661417459575529E-4	2.0965101178102412E-5	0.0	4.89185694155723E-5	1.7470917648418677E-6
12	0.0011583218400901584	2.271219294294428E-5	0.0	5.066566118041417E-5	1.7470917648418677E-6
13	0.0012631473459806704	2.445928470778615E-5	0.0	5.066566118041417E-5	1.7470917648418677E-6
14	0.0013697199436360243	2.445928470778615E-5	0.0	5.94011200046235E-5	1.7470917648418677E-6
15	0.0029665618167014915	2.445928470778615E-5	0.0	6.464239529914911E-5	3.4941835296837354E-6
16	0.003122052983772418	2.445928470778615E-5	0.0	6.988367059367471E-5	3.4941835296837354E-6
17	0.0032845325179027113	2.7953468237469883E-5	0.0	7.512494588820032E-5	3.4941835296837354E-6
18	0.003509907355567312	2.7953468237469883E-5	0.0	8.036622118272591E-5	3.4941835296837354E-6
19	0.003829625148533374	2.970056000231175E-5	0.0	8.560749647725152E-5	3.4941835296837354E-6
20	0.019303616909737798	3.144765176715362E-5	0.0	8.560749647725152E-5	5.241275294525603E-6
21	0.019630323069763225	3.144765176715362E-5	0.0	9.259586353661899E-5	5.241275294525603E-6
22	0.019899375201548875	3.319474353199549E-5	0.0	9.609004706630273E-5	5.241275294525603E-6
23	0.02010378493803537	3.319474353199549E-5	0.0	9.78371388311446E-5	5.241275294525603E-6
24	0.02036235451923197	3.319474353199549E-5	0.0	1.030784141256702E-4	5.241275294525603E-6
25	0.020647130476901192	3.319474353199549E-5	0.0	1.0482550589051207E-4	5.241275294525603E-6
26	0.02092666515927589	3.494183529683736E-5	0.0	1.0482550589051207E-4	5.241275294525603E-6
27	0.021363438100486357	3.843601882652109E-5	0.0	1.083196894201958E-4	5.241275294525603E-6
28	0.022846719008837106	4.0183110591362956E-5	0.0	1.1006678118503766E-4	5.241275294525603E-6
29	0.023360363987700614	4.0183110591362956E-5	0.0	1.1705514824440514E-4	5.241275294525603E-6
30	0.024677671178391383	4.0183110591362956E-5	0.0	1.2054933177408887E-4	5.241275294525603E-6
31	0.060604866230599554	4.367729412104669E-5	0.0	1.2579060706861448E-4	5.241275294525603E-6
32	0.06136310405654092	4.367729412104669E-5	0.0	1.2753769883345635E-4	5.241275294525603E-6
33	0.061667098023623405	4.89185694155723E-5	0.0	1.3103188236314009E-4	5.241275294525603E-6
34	0.06196410362364652	4.89185694155723E-5	0.0	1.3103188236314009E-4	5.241275294525603E-6
35	0.06228731560014227	4.89185694155723E-5	0.0	1.3802024942250756E-4	5.241275294525603E-6
36	0.06269613507311526	4.89185694155723E-5	0.0	1.4326152471703317E-4	6.988367059367471E-6
37	0.06305778306843754	4.89185694155723E-5	0.0	1.467557082467169E-4	6.988367059367471E-6
38	0.0634403961649379	5.5906936474939766E-5	0.0	1.467557082467169E-4	6.988367059367471E-6
39	0.0638911458402671	5.7654028239781635E-5	0.0	1.572382588357681E-4	8.73545882420934E-6
>>END_MODULE
