##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5865513_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60661376
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816482237396	33.0	33.0	33.0	33.0	33.0
2	31.895129612622043	33.0	33.0	33.0	33.0	33.0
3	31.941080416639412	33.0	33.0	33.0	33.0	33.0
4	31.977827126770087	33.0	33.0	33.0	33.0	33.0
5	32.00123475603323	33.0	33.0	33.0	33.0	33.0
6	35.58785535956191	37.0	37.0	37.0	33.0	37.0
7	35.52417576218515	37.0	37.0	37.0	33.0	37.0
8	35.5313971941553	37.0	37.0	37.0	33.0	37.0
9	35.5248948029138	37.0	37.0	37.0	33.0	37.0
10	35.52454921233571	37.0	37.0	37.0	33.0	37.0
11	35.54043460867093	37.0	37.0	37.0	33.0	37.0
12	35.51166444031866	37.0	37.0	37.0	33.0	37.0
13	35.5223101928977	37.0	37.0	37.0	33.0	37.0
14	35.52318593960018	37.0	37.0	37.0	33.0	37.0
15	35.520533807212026	37.0	37.0	37.0	33.0	37.0
16	35.52476925350325	37.0	37.0	37.0	33.0	37.0
17	35.53155142738602	37.0	37.0	37.0	33.0	37.0
18	35.529259573670075	37.0	37.0	37.0	33.0	37.0
19	35.525774176306186	37.0	37.0	37.0	33.0	37.0
20	35.52665059889179	37.0	37.0	37.0	33.0	37.0
21	35.52699398048603	37.0	37.0	37.0	33.0	37.0
22	35.51768988886767	37.0	37.0	37.0	33.0	37.0
23	35.51015700665939	37.0	37.0	37.0	33.0	37.0
24	35.49059320711749	37.0	37.0	37.0	33.0	37.0
25	35.49737528209054	37.0	37.0	37.0	33.0	37.0
26	35.45082513459635	37.0	37.0	37.0	33.0	37.0
27	35.45268264274124	37.0	37.0	37.0	33.0	37.0
28	35.4609717392497	37.0	37.0	37.0	33.0	37.0
29	35.42909267340062	37.0	37.0	37.0	33.0	37.0
30	35.453913739114654	37.0	37.0	37.0	33.0	37.0
31	35.45425909890339	37.0	37.0	37.0	33.0	37.0
32	35.453393968511364	37.0	37.0	37.0	33.0	37.0
33	35.44831345401727	37.0	37.0	37.0	33.0	37.0
34	35.44739194508215	37.0	37.0	37.0	33.0	37.0
35	35.37810866670746	37.0	37.0	37.0	33.0	37.0
36	35.39920960579595	37.0	37.0	37.0	33.0	37.0
37	35.39105957636042	37.0	37.0	37.0	33.0	37.0
38	35.39626916804525	37.0	37.0	37.0	33.0	37.0
39	35.37939559762047	37.0	37.0	37.0	33.0	37.0
40	35.36215312689247	37.0	37.0	37.0	33.0	37.0
41	35.349553940220545	37.0	37.0	37.0	33.0	37.0
42	35.336489284384186	37.0	37.0	37.0	33.0	37.0
43	35.31169871583526	37.0	37.0	37.0	33.0	37.0
44	35.28067119677602	37.0	37.0	37.0	33.0	37.0
45	35.244373256551256	37.0	37.0	37.0	33.0	37.0
46	35.154863697783576	37.0	37.0	37.0	33.0	37.0
47	35.128227012193065	37.0	37.0	37.0	33.0	37.0
48	35.02314457885031	37.0	37.0	37.0	33.0	37.0
49	34.872749226789715	37.0	37.0	37.0	33.0	37.0
50	34.30991252160188	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	377837.0
3	51227.0
4	30221.0
5	36395.0
6	36293.0
7	35159.0
8	32496.0
9	31447.0
10	31524.0
11	30970.0
12	32946.0
13	33526.0
14	36182.0
15	38309.0
16	41745.0
17	46526.0
18	51437.0
19	59377.0
20	69215.0
21	83994.0
22	110427.0
23	158329.0
24	212888.0
25	273210.0
26	345556.0
27	427896.0
28	529023.0
29	650849.0
30	807794.0
31	1013897.0
32	1311845.0
33	1799360.0
34	2772096.0
35	6314861.0
36	4.2746519E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.65869099722155	30.006035434818585	27.94877258423769	22.386500983722172
2	22.46519921347141	30.085599242205497	30.501175911102024	16.948025633221068
3	21.229093791839375	28.00050107060047	31.054326414939837	19.716078722620324
4	20.94875457621321	27.539405617088835	30.749602721940988	20.76223708475696
5	24.081568110411407	27.933543370549955	28.85555994730692	19.129328571731723
6	21.699072872397764	27.410268270724096	30.79138706014518	20.09927179673296
7	22.54850111454545	28.272244777007376	29.558275408041357	19.620978700405818
8	20.81822169975085	27.928779308639758	30.941866176530542	20.31113281507885
9	22.37718056625606	27.308707372542116	30.174004739484516	20.140107321717306
10	21.30328529438711	29.309932772973706	29.504061040675055	19.88272089196413
11	23.578119590472756	27.620752483928086	28.66163568876314	20.13949223683602
12	21.437244353045397	28.874778617223047	28.685726616049656	21.002250413681903
13	23.19272698105622	27.65449081789984	28.716467426491487	20.436314774552454
14	22.49318903029519	27.78161443694893	29.095083761195372	20.6301127715605
15	22.24771271710666	27.908660937628554	28.473320308240517	21.37030603702427
16	22.833671326068313	27.753029386483792	28.575140967802405	20.83815831964549
17	22.094894594738854	27.861435893588034	29.283863125482185	20.75980638619093
18	22.664183174501925	27.761522703977615	28.592259985028473	20.982034136491983
19	21.90574268384923	27.903220725655085	29.334124837773057	20.85691175272262
20	21.721332915159778	28.59926873807276	28.68244920003164	20.996949146735822
21	22.315842729683137	28.005197283329565	28.647769563935622	21.03119042305168
22	22.26170779933928	27.976794084471983	28.75080864511446	21.010689471074272
23	22.404567581369932	28.055085783558603	28.62795964204899	20.912386993022476
24	21.613416292313232	28.728728130127163	28.507450513974465	21.150405063585143
25	21.611140422773175	28.914330178081315	28.445195457354274	21.029333941791233
26	21.716077862386225	28.89413616114198	28.380855770967667	21.008930205504125
27	22.258717587889755	28.128676243302326	28.360192319555694	21.252413849252232
28	21.60158649355217	28.771463879676634	28.545854507289793	21.081095119481407
29	22.27347223516827	28.31352095100493	28.44181486481963	20.97119194900717
30	21.673297740181624	28.236078937586367	28.78755687178663	21.30306645044538
31	22.341237378895585	28.251233966987026	28.214527699139847	21.193000954977546
32	21.794647951910743	28.264524875535667	28.68930336816241	21.25152380439118
33	21.788482181295628	28.857860222125854	27.96233280313253	21.39132479344599
34	22.470554190786416	28.18369311800599	28.02826194902517	21.317490742182425
35	21.76666431642655	28.766192180891576	28.0032090609308	21.46393444175108
36	21.88533663690933	28.09638571976427	28.671173438913183	21.34710420441321
37	21.789438751830705	28.186135847398315	28.104441338132236	21.919984062638747
38	21.74226519470767	28.252193204128012	28.74841892508603	21.257122676078282
39	21.732666540383377	28.150002535745962	28.100493934229608	22.016836989641053
40	22.513720476450803	28.160393530161983	27.94675797946926	21.379128013917956
41	22.358877130865714	28.14088599542374	28.00484286892475	21.49539400478579
42	21.855471899364655	28.051068022405694	28.573410348306766	21.52004972992288
43	22.4692855840913	28.184029899974362	27.957704063175925	21.388980452758418
44	22.588788564943055	28.291849038369694	27.790484535022802	21.32887786166445
45	21.902115153970733	28.172134824575966	28.529555953152837	21.39619406830046
46	21.68352937688816	28.2321193409198	28.011276824198518	22.07307445799352
47	22.334400681689264	28.15737134518183	28.103561599665472	21.404666373463428
48	21.78681875835286	28.18539104886938	27.93744816346195	22.090342029315806
49	21.769979556917882	28.311509691381296	27.94366410290991	21.974846648790916
50	22.28624693438772	28.202188726372977	28.017457142900582	21.49410719633872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165980.0
1	93590.0
2	21200.0
3	19431.5
4	17663.0
5	19575.5
6	21488.0
7	22526.0
8	23564.0
9	24524.5
10	25485.0
11	31423.5
12	37362.0
13	59188.5
14	81015.0
15	164298.0
16	247581.0
17	412668.5
18	577756.0
19	633254.0
20	688752.0
21	712696.5
22	736641.0
23	914053.5
24	1091466.0
25	1473532.5
26	1855599.0
27	2201446.0
28	2547293.0
29	2599011.0
30	2650729.0
31	2622387.5
32	2594046.0
33	2715606.5
34	2837167.0
35	3080698.0
36	3324229.0
37	3582449.0
38	3840669.0
39	3912565.0
40	3984461.0
41	4003346.5
42	4022232.0
43	3923101.0
44	3823970.0
45	3754676.5
46	3685383.0
47	3469977.5
48	3254572.0
49	3266322.0
50	3278072.0
51	3068204.0
52	2858336.0
53	2650402.0
54	2442468.0
55	2278476.5
56	2114485.0
57	2102724.5
58	2090964.0
59	1751867.5
60	1412771.0
61	1217589.5
62	1022408.0
63	886923.5
64	751439.0
65	632299.0
66	513159.0
67	476897.0
68	440635.0
69	413544.0
70	386453.0
71	341014.5
72	295576.0
73	273529.0
74	251482.0
75	196659.0
76	141836.0
77	125273.0
78	108710.0
79	112771.0
80	116832.0
81	100652.5
82	84473.0
83	76460.5
84	68448.0
85	55929.5
86	43411.0
87	51741.5
88	60072.0
89	40865.5
90	21659.0
91	11092.0
92	525.0
93	401.5
94	278.0
95	245.5
96	213.0
97	191.5
98	170.0
99	184.0
100	198.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2370223187815588
2	0.24376796200600526
3	0.2488255459289285
4	0.25284292924710444
5	0.25387488737479347
6	0.2438371988132943
7	0.23866422021155603
8	0.24417349187726964
9	0.24335418965768266
10	0.24530930521589225
11	0.24074132443022722
12	0.2411419088152567
13	0.23883896072519029
14	0.24029128518284845
15	0.2371014465613177
16	0.2216138321689241
17	0.21149701582766603
18	0.21067441661725578
19	0.20358423785177573
20	0.2009829120922018
21	0.20374579040211682
22	0.21359390199127698
23	0.20982379298484757
24	0.20863193080222908
25	0.2110370856078174
26	0.21645074453965568
27	0.21848169088680086
28	0.21829211391446182
29	0.2182509015291707
30	0.21256524085441122
31	0.21793768740095842
32	0.2202290960231433
33	0.23125258484080544
34	0.23405832403142324
35	0.23530128957180266
36	0.24324703745592582
37	0.23813670167983003
38	0.24129851587936282
39	0.2417337186680368
40	0.24095562883374091
41	0.2406918695678779
42	0.24018248448568
43	0.23407480898553967
44	0.24482794455569223
45	0.24102156865020669
46	0.2396945298438334
47	0.24426415912491006
48	0.23972914824747796
49	0.2447768411979313
50	0.24158370558557724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6.0661376E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.2699271445063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64582451933762	33.69517229566209
2	11.734954323677533	9.68601419964562
3	2.996860131026838	3.710405978092596
4	1.2221943200206906	2.0175948217473323
5	0.5957319589730307	1.2292907272235496
6	0.382008709170045	0.945928295760879
7	0.2579056203790988	0.7450622314242874
8	0.1805790926007092	0.596198879636186
9	0.13014888472719213	0.48341114915669714
>10	0.6959034622139911	5.167275244667923
>50	0.0572996231842317	1.681915707011472
>100	0.06786088326343416	6.147729125981659
>500	0.012699776131568983	3.6445338455339678
>1k	0.015747771899387298	15.01772616852603
>5k	0.0033518555939270806	9.542420975568424
>10k+	9.290678009647613E-4	5.689320354361645
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	347418	0.5727169789224695	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	105459	0.17384867761654466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2310510068218694E-4	3.296990823287622E-6	0.0	1.648495411643811E-6	0.0
2	3.857479263246518E-4	1.1539467881506677E-5	0.0	3.296990823287622E-6	0.0
3	4.483907519671166E-4	1.1539467881506677E-5	0.0	6.593981646575244E-6	0.0
4	4.929001280814995E-4	1.3187963293150488E-5	0.0	1.1539467881506677E-5	3.296990823287622E-6
5	5.505974674890328E-4	1.648495411643811E-5	0.0	1.3187963293150488E-5	4.945486234931433E-6
6	6.066463114849225E-4	1.648495411643811E-5	0.0	1.9781944939725733E-5	6.593981646575244E-6
7	6.857740912438254E-4	1.648495411643811E-5	0.0	2.4727431174657165E-5	6.593981646575244E-6
8	7.23689485711633E-4	1.813344952808192E-5	0.0	2.9672917409588597E-5	6.593981646575244E-6
9	8.044657608821798E-4	1.9781944939725733E-5	0.0	3.626689905616384E-5	6.593981646575244E-6
10	8.819450452294389E-4	2.1430440351369545E-5	0.0	4.6157871526026706E-5	8.242477058219054E-6
11	9.643698158116295E-4	2.1430440351369545E-5	0.0	5.110335776095814E-5	8.242477058219054E-6
12	0.0011275708615643668	2.4727431174657165E-5	0.0	5.9345834819177195E-5	9.890972469862866E-6
13	0.0012693414669657345	2.802442199794479E-5	0.0	6.429132105410863E-5	9.890972469862866E-6
14	0.0014210030448369652	2.802442199794479E-5	0.0	6.758831187739625E-5	9.890972469862866E-6
15	0.0025502224018129757	2.9672917409588597E-5	0.0	7.253379811232768E-5	1.1539467881506677E-5
16	0.0026722110622746176	3.296990823287622E-5	0.0	8.077627517054674E-5	1.3187963293150488E-5
17	0.00282881812638078	3.7915394467807653E-5	0.0	8.407326599383436E-5	1.3187963293150488E-5
18	0.0030216920895431054	3.9563889879451465E-5	0.0	9.561273387534104E-5	1.648495411643811E-5
19	0.0033250152452855668	4.286088070273909E-5	0.0	1.0220671552191628E-4	1.648495411643811E-5
20	0.014055071879675134	4.6157871526026706E-5	0.0	1.1539467881506678E-4	1.813344952808192E-5
21	0.014388067952827183	4.6157871526026706E-5	0.0	1.1539467881506678E-4	1.813344952808192E-5
22	0.0146633666865717	4.6157871526026706E-5	0.0	1.2363715587328583E-4	1.813344952808192E-5
23	0.014928774447846353	4.6157871526026706E-5	0.0	1.2693414669657344E-4	1.9781944939725733E-5
24	0.01517440026418128	4.780636693767052E-5	0.0	1.335281283431487E-4	2.3078935763013353E-5
25	0.01548102041074703	4.780636693767052E-5	0.0	1.4177060540136776E-4	2.3078935763013353E-5
26	0.015787640557312778	5.2751853172601954E-5	0.0	1.4341910081301157E-4	2.4727431174657165E-5
27	0.016305268116568936	5.2751853172601954E-5	0.0	1.500130824595868E-4	2.4727431174657165E-5
28	0.01762241595047234	5.604884399588958E-5	0.0	1.5660706410616204E-4	2.6375926586300977E-5
29	0.018090588647379183	5.769733940753339E-5	0.0	1.632010457527373E-4	2.9672917409588597E-5
30	0.01921321402270862	6.0994330230821007E-5	0.0	1.6649803657602493E-4	3.132141282123241E-5
31	0.046584831837642456	6.264282564246483E-5	0.0	1.7474051363424397E-4	3.461840364452003E-5
32	0.04717993868124587	6.264282564246483E-5	0.0	1.780375044575316E-4	3.626689905616384E-5
33	0.047544256167219155	6.593981646575244E-5	0.0	1.8133449528081921E-4	4.121238529109528E-5
34	0.04790692515778079	6.758831187739625E-5	0.0	1.8463148610410682E-4	4.121238529109528E-5
35	0.048302564056575306	6.758831187739625E-5	0.0	1.945224585739697E-4	4.121238529109528E-5
36	0.04868501499207667	6.758831187739625E-5	0.0	2.0441343104383257E-4	4.121238529109528E-5
37	0.04909713884498763	7.088530270068388E-5	0.0	2.1265590810205163E-4	4.286088070273909E-5
38	0.04956860853271775	7.253379811232768E-5	0.0	2.1430440351369543E-4	4.286088070273909E-5
39	0.04995930194527734	7.583078893561531E-5	0.0	2.2089838516027067E-4	4.6157871526026706E-5
>>END_MODULE
