##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5865512_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59408720
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.819607323638685	33.0	33.0	33.0	33.0	33.0
2	31.89895387411141	33.0	33.0	33.0	33.0	33.0
3	31.933029141176583	33.0	33.0	33.0	33.0	33.0
4	31.9635750946999	33.0	33.0	33.0	33.0	33.0
5	31.986956611756657	33.0	33.0	33.0	33.0	33.0
6	35.562470273724124	37.0	37.0	37.0	33.0	37.0
7	35.508225374995455	37.0	37.0	37.0	33.0	37.0
8	35.516504799295454	37.0	37.0	37.0	33.0	37.0
9	35.5315867098298	37.0	37.0	37.0	33.0	37.0
10	35.53952692466695	37.0	37.0	37.0	33.0	37.0
11	35.549235196449274	37.0	37.0	37.0	33.0	37.0
12	35.52960006544494	37.0	37.0	37.0	33.0	37.0
13	35.533091118610194	37.0	37.0	37.0	33.0	37.0
14	35.533017324729435	37.0	37.0	37.0	33.0	37.0
15	35.533531693663825	37.0	37.0	37.0	33.0	37.0
16	35.53212168180025	37.0	37.0	37.0	33.0	37.0
17	35.53738569018151	37.0	37.0	37.0	33.0	37.0
18	35.534334370442586	37.0	37.0	37.0	33.0	37.0
19	35.52826502574033	37.0	37.0	37.0	33.0	37.0
20	35.52909244636141	37.0	37.0	37.0	33.0	37.0
21	35.52597059825561	37.0	37.0	37.0	33.0	37.0
22	35.5129833128874	37.0	37.0	37.0	33.0	37.0
23	35.504023954732574	37.0	37.0	37.0	33.0	37.0
24	35.49771622751677	37.0	37.0	37.0	33.0	37.0
25	35.49641146956204	37.0	37.0	37.0	33.0	37.0
26	35.44716842914643	37.0	37.0	37.0	33.0	37.0
27	35.44770589233365	37.0	37.0	37.0	33.0	37.0
28	35.46093834373136	37.0	37.0	37.0	33.0	37.0
29	35.42478326414035	37.0	37.0	37.0	33.0	37.0
30	35.44854800776721	37.0	37.0	37.0	33.0	37.0
31	35.44902517677539	37.0	37.0	37.0	33.0	37.0
32	35.44854810876249	37.0	37.0	37.0	33.0	37.0
33	35.443519924347804	37.0	37.0	37.0	33.0	37.0
34	35.44195424510072	37.0	37.0	37.0	33.0	37.0
35	35.37576426490926	37.0	37.0	37.0	33.0	37.0
36	35.39453972413477	37.0	37.0	37.0	33.0	37.0
37	35.38666227786089	37.0	37.0	37.0	33.0	37.0
38	35.38779921533405	37.0	37.0	37.0	33.0	37.0
39	35.373600861961	37.0	37.0	37.0	33.0	37.0
40	35.35296017150344	37.0	37.0	37.0	33.0	37.0
41	35.341423346606355	37.0	37.0	37.0	33.0	37.0
42	35.32533262793745	37.0	37.0	37.0	33.0	37.0
43	35.30267669459972	37.0	37.0	37.0	33.0	37.0
44	35.271241410351884	37.0	37.0	37.0	33.0	37.0
45	35.236847637855185	37.0	37.0	37.0	33.0	37.0
46	35.15436132944793	37.0	37.0	37.0	33.0	37.0
47	35.12080617121527	37.0	37.0	37.0	33.0	37.0
48	35.02256863975524	37.0	37.0	37.0	33.0	37.0
49	34.86193270954163	37.0	37.0	37.0	33.0	37.0
50	34.28519220747392	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	360449.0
3	48960.0
4	29664.0
5	35127.0
6	34684.0
7	33613.0
8	31592.0
9	31309.0
10	31170.0
11	31127.0
12	32781.0
13	33676.0
14	36079.0
15	37692.0
16	41253.0
17	45923.0
18	51549.0
19	59448.0
20	68596.0
21	83739.0
22	110249.0
23	157402.0
24	212842.0
25	272015.0
26	345031.0
27	426059.0
28	524276.0
29	645001.0
30	796746.0
31	998739.0
32	1291627.0
33	1764822.0
34	2709040.0
35	6164846.0
36	4.1831594E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.85038203585562	31.517955136005433	26.558672596006545	22.0729902321324
2	22.75718625611344	30.33116851768667	30.202800255558728	16.70884497064117
3	21.0904638312646	28.64474637233001	30.726868451740657	19.537921344664735
4	21.159411201604055	28.591212791083098	30.23775932953149	20.011616677781358
5	23.132076595672153	29.03582751148612	28.61653132069002	19.21556457215171
6	21.699991568143282	28.358721793967035	29.389261061066403	20.552025576823286
7	22.39223971092449	28.6490070301492	28.951225222660536	20.00752803626578
8	20.7193630735388	28.233512951732248	30.23869218335396	20.808431791374993
9	21.595162809395358	27.801437022380128	29.991549964450385	20.611850203774132
10	21.345797919368913	29.026537634214094	29.35718065031584	20.27048379610115
11	22.546471924825955	28.321808978336293	28.810879055911016	20.32084004092674
12	21.39281762893777	29.06529555555968	28.860109781804827	20.681777033697728
13	22.330195966645856	28.295028476369744	28.84584367432496	20.52893188265944
14	22.077633609873565	28.299616725888647	28.970859369711267	20.651890294526517
15	21.91832709128311	28.264868079718873	28.83040763294062	20.986397196057396
16	22.053209263971638	28.177509408447055	28.857243981259355	20.91203734632195
17	21.88832337497987	28.27964002498003	29.009154766904626	20.822881833135472
18	22.00610184406467	28.22432485015703	28.75082599478414	21.01874731099416
19	21.81151212883858	28.307493302518367	28.9356203471204	20.945374221522652
20	21.698881877754246	28.52921997192525	28.742674889413472	21.02922326090703
21	21.827916486484053	28.33044855612203	28.712277148303862	21.129357809090056
22	21.829173432983723	28.29306729457819	28.811346320028807	21.06641295240928
23	21.972048560536123	28.33431477511545	28.677418500551504	21.016218163796925
24	21.686950133951935	28.55048783974516	28.572324977976894	21.190237048326015
25	21.665036910312573	28.694157791565157	28.538043607523182	21.102761690599092
26	21.779135789992207	28.65586159975861	28.50676860631452	21.058234003934665
27	21.81972314958755	28.355524393988752	28.505335077179954	21.319417379243742
28	21.670622543153396	28.502317966097547	28.63257772293981	21.19448176780925
29	21.852703894946206	28.553218623482124	28.532823346825765	21.06125413474591
30	21.71790047300452	28.408733487325282	28.482523312433077	21.390842727237118
31	21.92566318260544	28.47378563223158	28.338956675057354	21.261594510105628
32	21.83573121831647	28.48198236337675	28.38578051446784	21.29650590383894
33	21.819974322046242	28.606776816173312	28.136054822556122	21.437194039224323
34	21.992651161488013	28.449478759889406	28.185135862234077	21.372734216388505
35	21.758997425142038	28.59230209795043	28.196663685432156	21.45203679147538
36	21.845775661902977	28.369286679920634	28.35466897418196	21.430268683994427
37	21.765227164207978	28.47495875304346	28.237427779334862	21.522386303413697
38	21.74040320569712	28.495116269644182	28.43930230485453	21.32517821980416
39	21.729992702718906	28.435609699081603	28.231968530118827	21.60242906808066
40	22.02006223032564	28.406339553283694	28.114766092281524	21.458832124109144
41	21.897443377098696	28.391963631539692	28.202875566747025	21.507717424614583
42	21.808624685690386	28.350461060552405	28.257215797551645	21.583698456205564
43	22.02562420572779	28.462931500258964	28.080295627649864	21.43114866636338
44	22.057429956907917	28.53759177896693	28.001405607665873	21.40357265645928
45	21.872960162357348	28.417091304425814	28.269793143916544	21.4401553893003
46	21.74136297681397	28.43620279505943	28.204955839086875	21.61747838903972
47	21.887533503107157	28.430532560194226	28.25868549702716	21.423248439671458
48	21.785766230856165	28.430209857891107	28.11513280335951	21.668891107893216
49	21.727216590574812	28.59657081705802	28.103846923884213	21.572365668482956
50	21.85036696559918	28.485467036472915	28.156472705342704	21.507693292585195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158372.0
1	88910.5
2	19449.0
3	17910.0
4	16371.0
5	17953.5
6	19536.0
7	20758.5
8	21981.0
9	23957.5
10	25934.0
11	32940.0
12	39946.0
13	61323.5
14	82701.0
15	153691.0
16	224681.0
17	366274.5
18	507868.0
19	567327.5
20	626787.0
21	667397.5
22	708008.0
23	875270.5
24	1042533.0
25	1393756.5
26	1744980.0
27	2052719.5
28	2360459.0
29	2411362.0
30	2462265.0
31	2475479.0
32	2488693.0
33	2623884.5
34	2759076.0
35	3031583.5
36	3304091.0
37	3552745.5
38	3801400.0
39	3909371.0
40	4017342.0
41	4055045.5
42	4092749.0
43	4019697.0
44	3946645.0
45	3896453.5
46	3846262.0
47	3663695.5
48	3481129.0
49	3448296.0
50	3415463.0
51	3205618.0
52	2995773.0
53	2770782.5
54	2545792.0
55	2344110.0
56	2142428.0
57	1979479.5
58	1816531.0
59	1582816.5
60	1349102.0
61	1148955.0
62	948808.0
63	809067.5
64	669327.0
65	549648.0
66	429969.0
67	384077.5
68	338186.0
69	301765.0
70	265344.0
71	229068.5
72	192793.0
73	178037.5
74	163282.0
75	124679.5
76	86077.0
77	75538.5
78	65000.0
79	63389.5
80	61779.0
81	54612.0
82	47445.0
83	41071.0
84	34697.0
85	26492.0
86	18287.0
87	17694.5
88	17102.0
89	11240.5
90	5379.0
91	2840.5
92	302.0
93	237.0
94	172.0
95	157.0
96	142.0
97	139.5
98	137.0
99	141.0
100	145.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23841449538047613
2	0.24513068115253112
3	0.2499734045776445
4	0.2538583561470438
5	0.254718499237149
6	0.24470986750766555
7	0.23947326251095796
8	0.2448950255114064
9	0.2441796423151349
10	0.2462870770486218
11	0.24204864201753548
12	0.242203501438846
13	0.23996982261189936
14	0.24165476044594128
15	0.23835053170645656
16	0.22252625540493046
17	0.21236276425413642
18	0.21151440394608736
19	0.2045322639504773
20	0.20174479436688755
21	0.20476118657328418
22	0.21433217211210745
23	0.21072664080289896
24	0.20938845341222637
25	0.21180392373375492
26	0.21703716222130354
27	0.21978423369498618
28	0.21923212619292254
29	0.2193095559035778
30	0.21349391133153517
31	0.21867328567254102
32	0.2209658784097688
33	0.23168652682636487
34	0.23479718128921143
35	0.23600575807726543
36	0.24402814940298326
37	0.23889253968104343
38	0.24235836086015655
39	0.24269332852146958
40	0.24192408117865524
41	0.2419543797610856
42	0.24156554795322976
43	0.2353846371374438
44	0.24591507778656063
45	0.24220855120258442
46	0.24083164895658415
47	0.24501453658654823
48	0.2405370794051782
49	0.2456120919622574
50	0.2426798624848339
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5.940872E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.35261624308117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3590168352318	40.15280335783575
2	12.429055440516892	12.268128068396209
3	3.109435139666124	4.603762775420811
4	1.1600695780423236	2.2900987480158363
5	0.5597660351983762	1.3812959160528266
6	0.3049525532484938	0.9030123799692416
7	0.21001334706819627	0.7255295686647195
8	0.14028409046367227	0.553870950533064
9	0.09647023689395538	0.428495272227586
>10	0.521742420811704	4.59695362343101
>50	0.04186173962434647	1.4642765341337125
>100	0.04654718920400036	4.815484814490656
>500	0.006728891069485695	2.311228136911466
>1k	0.0115415042383217	13.983799954133419
>5k	0.0022066209508468876	7.342554958968906
>10k+	3.083777711787641E-4	2.178704940814654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	103731	0.17460568078221514	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	79512	0.13383893812221506	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3466036635699272E-4	3.366509158924818E-6	0.0	8.416272897312045E-6	0.0
2	1.8684125832032738E-4	5.049763738387227E-6	0.0	1.1782782056236863E-5	0.0
3	2.558546960782862E-4	5.049763738387227E-6	0.0	1.514929121516168E-5	0.0
4	2.794202601907599E-4	6.733018317849636E-6	0.0	2.1882309533011315E-5	0.0
5	3.3160115215409455E-4	8.416272897312045E-6	0.0	2.3565564112473726E-5	0.0
6	3.652662437433427E-4	1.0099527476774454E-5	0.0	2.5248818691936133E-5	0.0
7	4.022978444915157E-4	1.0099527476774454E-5	0.0	2.861532785086095E-5	0.0
8	4.4437920897807596E-4	1.1782782056236863E-5	0.0	3.534834616871059E-5	0.0
9	5.066596284181851E-4	1.3466036635699272E-5	0.0	4.208136448656022E-5	0.0
10	5.63890284119907E-4	1.514929121516168E-5	0.0	4.208136448656022E-5	0.0
11	6.295372127189409E-4	1.514929121516168E-5	0.0	4.544787364548504E-5	0.0
12	7.389487603839975E-4	1.514929121516168E-5	0.0	4.881438280440986E-5	0.0
13	8.214282347776556E-4	1.514929121516168E-5	0.0	5.0497637383872266E-5	0.0
14	9.173737458070128E-4	1.514929121516168E-5	0.0	5.386414654279709E-5	0.0
15	0.0013920515372154122	1.683254579462409E-5	0.0	6.059716486064672E-5	0.0
16	0.0014879970482447695	1.683254579462409E-5	0.0	6.396367401957154E-5	0.0
17	0.0015856258138535891	2.1882309533011315E-5	0.0	6.901343775795876E-5	0.0
18	0.0016984038706775706	2.1882309533011315E-5	0.0	7.74297106552708E-5	0.0
19	0.0018801953652595107	2.5248818691936133E-5	0.0	8.584598355258286E-5	3.366509158924818E-6
20	0.005891391028118431	2.861532785086095E-5	0.0	8.584598355258286E-5	5.049763738387227E-6
21	0.006049616958587897	3.029858243032336E-5	0.0	9.257900187043249E-5	5.049763738387227E-6
22	0.006228041944010913	3.198183700978577E-5	0.0	9.42622564498949E-5	5.049763738387227E-6
23	0.006386267874480379	3.366509158924818E-5	0.0	1.0772829308559417E-4	5.049763738387227E-6
24	0.006539444041211459	3.534834616871059E-5	0.0	1.1109480224451899E-4	5.049763738387227E-6
25	0.006746484354485335	3.87148553276354E-5	0.0	1.1614456598290622E-4	5.049763738387227E-6
26	0.006933325612805662	4.0398109907097815E-5	0.0	1.1951107514183103E-4	5.049763738387227E-6
27	0.007214429127575884	4.208136448656022E-5	0.0	1.2119432972129345E-4	5.049763738387227E-6
28	0.007887730959360848	4.208136448656022E-5	0.0	1.2456083888021826E-4	5.049763738387227E-6
29	0.008106554054690962	4.208136448656022E-5	1.683254579462409E-6	1.279273480391431E-4	5.049763738387227E-6
30	0.0085526165182485	4.208136448656022E-5	3.366509158924818E-6	1.329771117775303E-4	6.733018317849636E-6
31	0.018192615494829714	4.208136448656022E-5	3.366509158924818E-6	1.4139338467484235E-4	6.733018317849636E-6
32	0.018556198483993595	4.544787364548504E-5	6.733018317849636E-6	1.4644314841322958E-4	6.733018317849636E-6
33	0.018793537379697797	4.881438280440986E-5	6.733018317849636E-6	1.514929121516168E-4	6.733018317849636E-6
34	0.01905612509409393	5.218089196333467E-5	6.733018317849636E-6	1.531761667310792E-4	6.733018317849636E-6
35	0.019296830498957057	5.218089196333467E-5	6.733018317849636E-6	1.683254579462409E-4	6.733018317849636E-6
36	0.019551001940455878	5.386414654279709E-5	6.733018317849636E-6	1.7169196710516572E-4	6.733018317849636E-6
37	0.019835471964385027	5.5547401122259494E-5	6.733018317849636E-6	1.7505847626409052E-4	6.733018317849636E-6
38	0.020116575479155248	5.5547401122259494E-5	6.733018317849636E-6	1.8010824000247775E-4	8.416272897312045E-6
39	0.02044481012215042	5.72306557017219E-5	8.416272897312045E-6	1.8684125832032738E-4	1.0099527476774454E-5
>>END_MODULE
