##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5865512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59408720
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41561110894158	33.0	33.0	33.0	33.0	33.0
2	32.49826151783778	33.0	33.0	33.0	33.0	33.0
3	32.50750504303072	33.0	33.0	33.0	33.0	33.0
4	32.52707329833061	33.0	33.0	33.0	33.0	33.0
5	32.538714451346536	33.0	33.0	33.0	33.0	33.0
6	35.97598416528752	37.0	37.0	37.0	33.0	37.0
7	36.13021877933071	37.0	37.0	37.0	37.0	37.0
8	36.22817332203084	37.0	37.0	37.0	37.0	37.0
9	36.22678835699541	37.0	37.0	37.0	37.0	37.0
10	36.26782221532462	37.0	37.0	37.0	37.0	37.0
11	36.275920773920056	37.0	37.0	37.0	37.0	37.0
12	36.1923649928832	37.0	37.0	37.0	37.0	37.0
13	36.268838783262794	37.0	37.0	37.0	37.0	37.0
14	36.29274379249376	37.0	37.0	37.0	37.0	37.0
15	36.29010505528481	37.0	37.0	37.0	37.0	37.0
16	36.285711777664964	37.0	37.0	37.0	37.0	37.0
17	36.29878300693905	37.0	37.0	37.0	37.0	37.0
18	36.3011035080372	37.0	37.0	37.0	37.0	37.0
19	36.267331496117066	37.0	37.0	37.0	37.0	37.0
20	36.28623363708223	37.0	37.0	37.0	37.0	37.0
21	36.293077447216504	37.0	37.0	37.0	37.0	37.0
22	36.20530098948437	37.0	37.0	37.0	37.0	37.0
23	36.169003355063026	37.0	37.0	37.0	37.0	37.0
24	36.199782405680516	37.0	37.0	37.0	37.0	37.0
25	36.2202762995062	37.0	37.0	37.0	37.0	37.0
26	36.19420946958628	37.0	37.0	37.0	37.0	37.0
27	36.201852825645794	37.0	37.0	37.0	37.0	37.0
28	36.190160367030295	37.0	37.0	37.0	37.0	37.0
29	36.070922736594895	37.0	37.0	37.0	37.0	37.0
30	36.14456694236132	37.0	37.0	37.0	37.0	37.0
31	36.16091529997617	37.0	37.0	37.0	37.0	37.0
32	36.16658037742607	37.0	37.0	37.0	37.0	37.0
33	36.15170276350004	37.0	37.0	37.0	37.0	37.0
34	36.12922786419233	37.0	37.0	37.0	37.0	37.0
35	36.12983440814749	37.0	37.0	37.0	37.0	37.0
36	36.112052961248786	37.0	37.0	37.0	37.0	37.0
37	36.037862017562404	37.0	37.0	37.0	37.0	37.0
38	36.012163955055755	37.0	37.0	37.0	37.0	37.0
39	35.97547001517622	37.0	37.0	37.0	37.0	37.0
40	35.94534564959488	37.0	37.0	37.0	37.0	37.0
41	35.85740361010976	37.0	37.0	37.0	37.0	37.0
42	35.871416384665416	37.0	37.0	37.0	37.0	37.0
43	35.864452373321626	37.0	37.0	37.0	37.0	37.0
44	35.787955842172664	37.0	37.0	37.0	37.0	37.0
45	35.73084695310722	37.0	37.0	37.0	37.0	37.0
46	35.72270769341605	37.0	37.0	37.0	37.0	37.0
47	35.62200347019764	37.0	37.0	37.0	33.0	37.0
48	35.60057585822418	37.0	37.0	37.0	33.0	37.0
49	35.4643914563384	37.0	37.0	37.0	37.0	37.0
50	34.86380102112956	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7383.0
3	2527.0
4	3247.0
5	3984.0
6	3360.0
7	3708.0
8	3820.0
9	4146.0
10	4808.0
11	5910.0
12	7476.0
13	9422.0
14	12350.0
15	14699.0
16	17757.0
17	20959.0
18	24217.0
19	28519.0
20	32882.0
21	40551.0
22	48405.0
23	62384.0
24	85647.0
25	120347.0
26	174139.0
27	246935.0
28	347118.0
29	473883.0
30	640133.0
31	859180.0
32	1166993.0
33	1658799.0
34	2651311.0
35	6739003.0
36	4.3882718E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.25410361799543	30.798474252570013	26.330461018700614	22.616961110733943
2	22.913416924983764	29.259123954956383	30.40667619996234	17.420782920097516
3	20.501015675813246	27.88709300587523	31.619237041296294	19.992654277015227
4	21.060465013874413	28.37500931682727	30.491693160570122	20.07283250872819
5	23.054844474009876	28.739331532475365	28.63237416998717	19.57344982352759
6	21.938861837117514	28.183296997477814	29.002165002040105	20.87567616336457
7	22.576670562839933	28.58736225927776	28.46354036915793	20.372426808724377
8	20.794159510590365	27.978279956208446	29.941835138006677	21.28572539519451
9	21.406860811005522	27.567326816669336	30.01099501891305	21.01481735341209
10	21.371571378747092	28.453203502785453	29.483799684625424	20.691425433842035
11	22.491991074710917	28.08924009808661	28.746418034254905	20.672350792947565
12	21.422949358276025	28.829957959033624	28.69730066562619	21.049792017064163
13	22.277978720968907	28.108042051739208	28.773750722116215	20.84022850517567
14	22.049111645563144	28.058695760487684	28.932643221399147	20.959549372550022
15	21.864547830688828	27.829382622618365	29.15468806599435	21.151381480698454
16	21.783000879332192	27.902679943281054	29.128565638175672	21.185753539211078
17	21.850280564873305	28.034768296640628	28.906620105600663	21.208331032885408
18	21.82657844347284	28.136590260594087	28.81659990766338	21.220231388269696
19	21.919093695336308	28.062683390586436	28.86410614468718	21.154116769390082
20	21.660415755779773	28.138170056814094	28.84495235754711	21.356461829859022
21	21.62626799567471	28.20665047151327	28.82205002901931	21.345031503792708
22	21.859838084375493	28.010017721304216	28.89070998331558	21.239434211004713
23	22.02719398768396	28.076790747216908	28.73543311486933	21.160582150229796
24	21.668234898849867	28.31213498624444	28.684139298069372	21.335490816836316
25	21.667270393975834	28.433871323940323	28.685649177427152	21.21320910465669
26	21.759630572750936	28.237114012892384	28.801595119369683	21.201660294986997
27	21.761689193101617	28.087105731279856	28.69978009962174	21.451424975996787
28	21.638303265917866	28.07319194892602	28.819738920481708	21.46876586467441
29	21.648510521687726	28.288288318617198	28.727308045014265	21.335893114680808
30	21.814088908160283	28.17509113140293	28.533615940555528	21.477204019881256
31	21.712201508465423	28.33706903633002	28.58098777418534	21.369741681019217
32	21.893691650707588	28.25530585910204	28.476276820751085	21.374725669439286
33	21.72636777900618	28.37017023763515	28.400780222162673	21.502681761195998
34	21.763549189411926	28.232885677388776	28.422726495369705	21.580838637829597
35	21.66691186075041	28.243892479083875	28.44667415827172	21.642521501894
36	21.908055601746877	28.1468058832923	28.447834138257406	21.497304376703422
37	21.723778933462967	28.233643141949532	28.47467846471023	21.56789945987727
38	21.658212801083746	28.28683566991512	28.55177657421335	21.50317495478778
39	21.661498514022856	28.20770923864375	28.504913083466537	21.625879163866855
40	21.78617044770532	28.317821020213866	28.402655367764197	21.49335316431662
41	21.71364910740376	28.335693817338598	28.440833264880983	21.50982381037666
42	21.869035387397677	28.13278926056646	28.392784762910228	21.605390589125637
43	21.792240263718863	28.219668762430832	28.504919816484854	21.48317115736545
44	21.85784006118967	28.419876745366672	28.27137834311192	21.450904850331735
45	21.817346005771544	28.19081609568427	28.520829938769932	21.471007959774255
46	21.674530270977055	28.166376922445057	28.540827003173945	21.618265803403943
47	21.696693475508855	28.17593698882114	28.654697373696514	21.472672161973495
48	21.711351464902794	28.20368794345342	28.40333035285056	21.68163023879323
49	21.763279868679213	28.376851075061033	28.40075497334398	21.45911408291577
50	21.659256453795056	28.25596269854796	28.569374009758807	21.515406837898173
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46461.0
1	29638.5
2	12816.0
3	15092.0
4	17368.0
5	22041.5
6	26715.0
7	32163.5
8	37612.0
9	31586.5
10	25561.0
11	29701.0
12	33841.0
13	53757.5
14	73674.0
15	144229.5
16	214785.0
17	354239.0
18	493693.0
19	552626.5
20	611560.0
21	649788.0
22	688016.0
23	853228.0
24	1018440.0
25	1374735.0
26	1731030.0
27	2035480.5
28	2339931.0
29	2382332.0
30	2424733.0
31	2436370.0
32	2448007.0
33	2583540.0
34	2719073.0
35	2997847.0
36	3276621.0
37	3533503.0
38	3790385.0
39	3903574.0
40	4016763.0
41	4054498.5
42	4092234.0
43	4021798.0
44	3951362.0
45	3907272.0
46	3863182.0
47	3683260.0
48	3503338.0
49	3477352.0
50	3451366.0
51	3242687.5
52	3034009.0
53	2809122.0
54	2584235.0
55	2384851.0
56	2185467.0
57	2020381.0
58	1855295.0
59	1624999.0
60	1394703.0
61	1187291.0
62	979879.0
63	834516.0
64	689153.0
65	564679.5
66	440206.0
67	394051.5
68	347897.0
69	311324.0
70	274751.0
71	236268.5
72	197786.0
73	184110.5
74	170435.0
75	129554.0
76	88673.0
77	77311.5
78	65950.0
79	64848.0
80	63746.0
81	56637.5
82	49529.0
83	42727.0
84	35925.0
85	27489.5
86	19054.0
87	18504.0
88	17954.0
89	11691.0
90	5428.0
91	2741.5
92	55.0
93	32.0
94	9.0
95	8.5
96	8.0
97	5.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05637724562993446
2	0.0013348208815136902
3	0.0
4	1.4139338467484235E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	6.733018317849636E-6
19	0.0
20	7.57464560758084E-5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.5149291215161679E-5
33	0.0
34	0.0
35	0.0
36	1.8515800374086497E-5
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	1.4644314841322958E-4
48	0.0
49	0.0
50	0.0014223501196457356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5.940872E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.246276989826626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52440738443275	38.85002863110983
2	12.974266386429909	12.519200996389888
3	3.26185265337324	4.7211673984393885
4	1.2017108291488736	2.319122940991631
5	0.6102321452550398	1.4720714554035381
6	0.31745204481351175	0.9189527571035724
7	0.20794691179964173	0.7022865014105193
8	0.13786345466179842	0.5321118736310025
9	0.11043078319916592	0.47950867389874063
>10	0.5405502812439811	4.616461897134146
>50	0.04391288363358331	1.4998851191105849
>100	0.04749712913007884	4.818112887914356
>500	0.0070854452996154515	2.3793673518569425
>1k	0.01207327409729907	14.338386900862222
>5k	0.0024018930376165574	7.832850030450741
>10k+	3.1650044372796344E-4	2.0004845842930483
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCCAAGTATCTCGT	71679	0.12065400500128601	Illumina Paired End PCR Primer 2 (97% over 36bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4139338467484235E-4	0.0	0.0	1.0099527476774454E-5	0.0
2	1.9357427663817703E-4	0.0	0.0	1.514929121516168E-5	0.0
3	2.67637478134523E-4	0.0	0.0	2.0199054953548908E-5	0.0
4	2.9625280598538395E-4	1.683254579462409E-6	0.0	2.1882309533011315E-5	0.0
5	3.6694949832280515E-4	1.683254579462409E-6	0.0	2.6932073271398544E-5	0.0
6	4.1239737196829016E-4	1.683254579462409E-6	0.0	2.6932073271398544E-5	0.0
7	4.5111222729592556E-4	1.683254579462409E-6	0.0	2.6932073271398544E-5	1.683254579462409E-6
8	4.999266101003354E-4	3.366509158924818E-6	0.0	2.6932073271398544E-5	1.683254579462409E-6
9	5.689400478582942E-4	6.733018317849636E-6	0.0	2.6932073271398544E-5	1.683254579462409E-6
10	6.430032493546402E-4	1.1782782056236863E-5	0.0	3.198183700978577E-5	1.683254579462409E-6
11	7.069669233742117E-4	1.3466036635699272E-5	0.0	4.544787364548504E-5	1.683254579462409E-6
12	8.3152776225443E-4	1.514929121516168E-5	0.0	5.5547401122259494E-5	1.683254579462409E-6
13	9.257900187043249E-4	1.8515800374086497E-5	0.0	6.901343775795876E-5	1.683254579462409E-6
14	0.0010284685480515317	1.8515800374086497E-5	0.0	7.911296523473322E-5	3.366509158924818E-6
15	0.0014997798303010062	2.0199054953548908E-5	1.683254579462409E-6	8.921249271150767E-5	3.366509158924818E-6
16	0.0016007751050687508	2.0199054953548908E-5	1.683254579462409E-6	9.089574729097007E-5	3.366509158924818E-6
17	0.0017068201435748826	2.3565564112473726E-5	1.683254579462409E-6	9.257900187043249E-5	3.366509158924818E-6
18	0.0018212814549783264	2.5248818691936133E-5	1.683254579462409E-6	9.762876560881972E-5	3.366509158924818E-6
19	0.002008122713298654	2.5248818691936133E-5	1.683254579462409E-6	9.931202018828212E-5	3.366509158924818E-6
20	0.005936838901763916	2.6932073271398544E-5	1.683254579462409E-6	1.0099527476774453E-4	5.049763738387227E-6
21	0.006108530868869082	2.6932073271398544E-5	1.683254579462409E-6	1.0772829308559417E-4	6.733018317849636E-6
22	0.006285272599712635	2.6932073271398544E-5	1.683254579462409E-6	1.1109480224451899E-4	6.733018317849636E-6
23	0.006451914803079413	2.6932073271398544E-5	1.683254579462409E-6	1.1277805682398139E-4	6.733018317849636E-6
24	0.006608457478969417	2.861532785086095E-5	1.683254579462409E-6	1.1951107514183103E-4	6.733018317849636E-6
25	0.0068138145376638315	2.861532785086095E-5	1.683254579462409E-6	1.2287758430075586E-4	6.733018317849636E-6
26	0.007017488341778783	3.198183700978577E-5	1.683254579462409E-6	1.2624409345968066E-4	8.416272897312045E-6
27	0.007300275111128468	3.198183700978577E-5	1.683254579462409E-6	1.312938571980679E-4	8.416272897312045E-6
28	0.007983676470390205	3.366509158924818E-5	1.683254579462409E-6	1.312938571980679E-4	8.416272897312045E-6
29	0.00821764885693548	3.7031600748172994E-5	1.683254579462409E-6	1.3466036635699272E-4	1.0099527476774454E-5
30	0.00867886061170818	4.208136448656022E-5	1.683254579462409E-6	1.4644314841322958E-4	1.0099527476774454E-5
31	0.01947525548438007	4.208136448656022E-5	1.683254579462409E-6	1.498096575721544E-4	1.0099527476774454E-5
32	0.019864087292235887	4.544787364548504E-5	1.683254579462409E-6	1.514929121516168E-4	1.0099527476774454E-5
33	0.020101426187940086	4.713112822494745E-5	1.683254579462409E-6	1.5990918504892883E-4	1.0099527476774454E-5
34	0.020342131592803212	5.0497637383872266E-5	1.683254579462409E-6	1.6327569420785366E-4	1.0099527476774454E-5
35	0.020594619779722573	5.0497637383872266E-5	1.683254579462409E-6	1.700087125257033E-4	1.0099527476774454E-5
36	0.020845424712062473	5.5547401122259494E-5	1.683254579462409E-6	1.7842498542301535E-4	1.0099527476774454E-5
37	0.02115009379094517	5.891391028118431E-5	1.683254579462409E-6	1.8010824000247775E-4	1.0099527476774454E-5
38	0.02143119730571539	5.891391028118431E-5	1.683254579462409E-6	1.885245128997898E-4	1.0099527476774454E-5
39	0.021757748694131097	6.733018317849636E-5	3.366509158924818E-6	2.0030729495602666E-4	1.1782782056236863E-5
>>END_MODULE
