[2019-06-18 11:07:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-06-18 11:07:57] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-06-18 11:07:57] Checking for Bowtie index files (genome).. [2019-06-18 11:07:57] Checking for reference FASTA file [2019-06-18 11:07:57] Generating SAM header for Bowtie2Index/genome [2019-06-18 11:08:03] Reading known junctions from GTF file [2019-06-18 11:08:07] Preparing reads left reads: min. length=50, max. length=50, 68322598 kept reads (117578 discarded) right reads: min. length=50, max. length=50, 68129352 kept reads (310824 discarded) [2019-06-18 11:40:19] Building transcriptome data files /scratch/6794466.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-06-18 11:40:40] Building Bowtie index from RefSeq_GeneBody.fa [2019-06-18 11:48:26] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-18 12:07:31] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-18 12:26:37] Resuming TopHat pipeline with unmapped reads [2019-06-18 12:26:38] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-18 12:55:56] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-06-18 12:59:36] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-06-18 13:05:18] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-18 13:35:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-06-18 13:40:38] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-06-18 13:48:21] Searching for junctions via segment mapping [2019-06-18 13:58:21] Retrieving sequences for splices [2019-06-18 14:00:48] Indexing splices [2019-06-18 14:01:09] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-06-18 14:01:43] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-06-18 14:02:38] Joining segment hits [2019-06-18 14:06:48] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-06-18 14:07:34] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-06-18 14:08:47] Joining segment hits [2019-06-18 14:13:13] Reporting output tracks ----------------------------------------------- [2019-06-18 15:19:45] A summary of the alignment counts can be found in /scratch/6794466.1.linga/tophat2/align_summary.txt [2019-06-18 15:19:45] Run complete: 04:11:47 elapsed