-----------------------
Start of variable list:
-----------------------
Sample_ID:
G4-MAA100039-3_11_M-1-1
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/00_Setup_Pipeline
BU_User:
kkarri
VM_DIR_FASTQC:
/net/waxman-server/mnt/data/waxmanlabvm_home/waxmanlab/FASTQC/SRR
OUTPUT_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC/Job_Summary
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Fri Oct 12 19:46:09 EDT 2018
Running on node : scc-tm2
Current directory : /restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC
Current job ID : 8791198
Current job name : Step_02_G4-MAA100039-3_11_M-1-1
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/8791198.1.linga

Loading required modules...


List files in scratch directory:

total 60M
drwx------   3 kkarri waxmanlab 4.0K Oct 12 19:46 .
drwxrwxrwt. 48 root   root      116K Oct 12 19:46 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 19:46 G4-MAA100039-3_11_M-1-1_FASTQC
-rw-r--r--   1 kkarri waxmanlab  29M Oct 12 19:46 SRR6576945_1.fastq.gz
-rw-r--r--   1 kkarri waxmanlab  31M Oct 12 19:46 SRR6576945_2.fastq.gz

Starting to run my commands


Unzip files:


Finished unzipping


Print first 20 lines of FASTQ file:

==> SRR6576945_1.fastq <==
@SRR6576945.1 1/1
TNTCATGATGGATCAGCACTGGCTGCCTATGGGGATGTGGTAGTTGTCACTGTCCAGTATCGCCTTGGGATCTTTGGCTTCCTCAGCACTGGAGACAAGC
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.2 2/1
ATCATTTATTGAACAGGAAGAGGAAGAATTTCATGAAAAATTGTCAAGGGTGTATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCT
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.3 3/1
GTTTAATCCAGATGTCAGCTGGGACAGAAAGAACAACCCAGAGCCATGGAACAAACTGGGTCCCAATGAACAATATAAGTTCTACTCTGTGAATGTGGAC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.4 4/1
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGG
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.5 5/1
CCCGAGAGGGTCATCATGGGCACCTTCACTGTGGTGGACTCGTCCACATGGAACTCAGCTTCCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

==> SRR6576945_2.fastq <==
@SRR6576945.1 1/2
NTCTGTGGAAGAGCCCAGCAGACATTGGAGACAGGAGCAGGGATGAGACAATAATGCCTCCAGCAGAGTTACCGAAGATAGTGACACAGTTGGGATCACC
+
#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.2 2/2
GTGGAGACTTCTCACCAGGACTCCACCATCATCCCTTCCTATCCATACAGCATCCCCAGTATAAATTCTGTGATCTGCATTCCATCCTGTCTTACTGAGA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6576945.3 3/2
GTTTAGAAGTCTGGGCCTTCTTTCTTCAGTTTGCTGTAGTCCACATTCACAGAGTAGAACTTATATTGTTCATTGGGACCCAGTTTGTTCCATGGCTCTG
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF8F
@SRR6576945.4 4/2
AAGTACGCCCGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA
+
FFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFF-FFFF-FFFFFFFFFFFFFFFFFFF-FFFFFFFFFFF
@SRR6576945.5 5/2
GAGGAAGCTGAGTTCCATGTGGACGAGTCCACCACAGTGAAGGTGCCCATGATGACCCTCTCGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAAA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Starting FASTQC command


Processing SRR6576945_1.fastq

Printing command:
fastqc --outdir SRR6576945_1.fastq

Analysis complete for SRR6576945_1.fastq

Processing SRR6576945_2.fastq

Printing command:
fastqc --outdir SRR6576945_2.fastq

Analysis complete for SRR6576945_2.fastq

Finished FASTQC command


Copy output to storage dir


List files in scratch

total 347M
drwx------   3 kkarri waxmanlab 4.0K Oct 12 19:46 .
drwxrwxrwt. 46 root   root      116K Oct 12 19:46 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 19:46 G4-MAA100039-3_11_M-1-1_FASTQC
-rw-r--r--   1 kkarri waxmanlab 173M Oct 12 19:46 SRR6576945_1.fastq
-rw-r--r--   1 kkarri waxmanlab 173M Oct 12 19:46 SRR6576945_2.fastq
==========================================================
Finished on : Fri Oct 12 19:46:34 EDT 2018
0 hours, 0 minutes and 25 seconds elapsed.
==========================================================