-----------------------
Start of variable list:
-----------------------
Sample_ID:
C12-MAA100042-3_9_M-1-1
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/00_Setup_Pipeline
BU_User:
kkarri
VM_DIR_FASTQC:
/net/waxman-server/mnt/data/waxmanlabvm_home/waxmanlab/FASTQC/SRR
OUTPUT_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC/Job_Summary
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Fri Oct 12 19:44:29 EDT 2018
Running on node : scc-kb4
Current directory : /restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC
Current job ID : 8791011
Current job name : Step_02_C12-MAA100042-3_9_M-1-1
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/8791011.1.linga

Loading required modules...


List files in scratch directory:

total 129M
drwx------    3 kkarri waxmanlab 4.0K Oct 12 19:44 .
drwxrwxrwt. 108 root   root      164K Oct 12 19:44 ..
drwxr-xr-x    2 kkarri waxmanlab 4.0K Oct 12 19:44 C12-MAA100042-3_9_M-1-1_FASTQC
-rw-r--r--    1 kkarri waxmanlab  63M Oct 12 19:44 SRR6575590_1.fastq.gz
-rw-r--r--    1 kkarri waxmanlab  66M Oct 12 19:44 SRR6575590_2.fastq.gz

Starting to run my commands


Unzip files:


Finished unzipping


Print first 20 lines of FASTQ file:

==> SRR6575590_1.fastq <==
@SRR6575590.1 1/1
TCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGATACATTTCGGATGTCTCACCTCCCTTCCTAATGATCTCCTCACACTCTTTCC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.2 2/1
ACCTTCTTCTCAACAATGTCAGGGGCATTTTGGAATCAACCAAGGCCAGGGACATCCCTGTGGTCATTGATGCGGACGGCCTGTGGCTCGTTGCTCAGCA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.3 3/1
GCAGCGGACCGTGGAGGAGTCACTGCACACCCGAGTATCGTTTGAGCAGATCCTCAGCCTCCCAGAGCTCAAGGCTAACCCTTTCAAGGAGCGAATCTGC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.4 4/1
GGTCAGTATTAATAACCCTTTTTTGTCAGCACTCTCCCAGAGTGTCCTCCTGAGCTGATGCTATGGACACTTAATTCACACTGTTGAGATGTGAGACTGT
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.5 5/1
TTTTTTTTTAGTAACTGCTTTAATCTGTCCTCACACTCTCCCTGCAGGAAGCTCTTTATAGAAAACCCAAATCCTCATCTTTGAGCTTGTCCTTTAGCCA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFF-FFFFFF

==> SRR6575590_2.fastq <==
@SRR6575590.1 1/2
NTCTTCGTGTGCTCCGCTCAATCCTGGAGGACCTGAGAAGCAAAATACAAAAATTAGAGTCTGACATCTCAGCTCAAATGGAATACTGCCGCACTCCATG
+
#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.2 2/2
GGTTCAGGTCGTGAAGAGCCTGCTGAAGGCAGTTCCTACCTCTGTGATCATGTCGGTAGTGGTTGTAGAGCGCCCGTACTTCTGGAAGGCCTGCCGGTTA
+
FFFFFFFFFFFFFFFFFFFFFF-FFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFF
@SRR6575590.3 3/2
GTCCAGGGTTCCATCGTCATCAAAGTCAAAGATGCGGAAGGCATAGTGTGACTTGATGTCTGGGGTTGCTGTGTCACTGAAGACACTCAGGAGGTCCAGG
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.4 4/2
GCTATGTTATCCTTTCTCTTGTAACAAACCCACCCCAGAGATAAAGCCTTTAATCAACCCAAGAAACTCCTGGGCTAAGTATCTGACAGTCTCACATCTC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575590.5 5/2
TGTCTGCAGAGACCAGAGGCGGCAACAGCTGGTACCCTCTTATGCCCGGGACTTCCACATCAACCTCTTCCAGGTGCTGCCCTGGCTAAAGGACAAGCTC
+
FF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFF-F-FFFF8F8FFF

Starting FASTQC command


Processing SRR6575590_1.fastq

Printing command:
fastqc --outdir SRR6575590_1.fastq

Analysis complete for SRR6575590_1.fastq

Processing SRR6575590_2.fastq

Printing command:
fastqc --outdir SRR6575590_2.fastq

Analysis complete for SRR6575590_2.fastq

Finished FASTQC command


Copy output to storage dir


List files in scratch

total 717M
drwx------    3 kkarri waxmanlab 4.0K Oct 12 19:44 .
drwxrwxrwt. 108 root   root      164K Oct 12 19:44 ..
drwxr-xr-x    2 kkarri waxmanlab 4.0K Oct 12 19:45 C12-MAA100042-3_9_M-1-1_FASTQC
-rw-r--r--    1 kkarri waxmanlab 358M Oct 12 19:44 SRR6575590_1.fastq
-rw-r--r--    1 kkarri waxmanlab 358M Oct 12 19:44 SRR6575590_2.fastq
==========================================================
Finished on : Fri Oct 12 19:45:27 EDT 2018
0 hours, 0 minutes and 58 seconds elapsed.
==========================================================