-----------------------
Start of variable list:
-----------------------
Sample_ID:
B3-MAA100138-3_56_F-1-1
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/00_Setup_Pipeline
BU_User:
kkarri
VM_DIR_FASTQC:
/net/waxman-server/mnt/data/waxmanlabvm_home/waxmanlab/FASTQC/SRR
OUTPUT_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC/Job_Summary
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Fri Oct 12 19:42:21 EDT 2018
Running on node : scc-ka3
Current directory : /restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/02_FASTQC
Current job ID : 8790976
Current job name : Step_02_B3-MAA100138-3_56_F-1-1
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/8790976.1.linga

Loading required modules...


List files in scratch directory:

total 64M
drwx------   3 kkarri waxmanlab 4.0K Oct 12 19:42 .
drwxrwxrwt. 89 root   root      108K Oct 12 19:42 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 19:42 B3-MAA100138-3_56_F-1-1_FASTQC
-rw-r--r--   1 kkarri waxmanlab  32M Oct 12 19:42 SRR6575753_1.fastq.gz
-rw-r--r--   1 kkarri waxmanlab  33M Oct 12 19:42 SRR6575753_2.fastq.gz

Starting to run my commands


Unzip files:


Finished unzipping


Print first 20 lines of FASTQ file:

==> SRR6575753_1.fastq <==
@SRR6575753.1 1/1
GCACTGCGGCCTTGAGGTGGCTTGAACGCTACTACTGCTTCCAGGGTAACAAGTTCCTGCGATTCAACCCTGTCACAGGAGAGGTGCCTCCCAGATACCC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.2 2/1
GAAGAAACATGTGCCAGCCTTCTTCAAAATGCAGATCTGTGAAAAAACGCTCAAAGTCTTGGACGGTGTAGTTGAAGTTTGGTGTGGAAATGAACACATG
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.3 3/1
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.4 4/1
GTACCAGACTGAGGCCTTCACAGCAGACTTCCAGCAGCCTACTGAGGCCAAAAACCTCATCAATGACTATGTGAGCAATCAGACCCAGGGGATGATCAAG
+
8FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.5 5/1
CTGCTTGGTGACTCGAAGGAACTGGTCGGCGAAGTCCTCGGCCGTGCCCATCAGGTCGTTCTGTCCCATGGCGGCGGCGGAAGGCTCAGCTGGCAGGTCC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

==> SRR6575753_2.fastq <==
@SRR6575753.1 1/2
NGCTATGCCATCCATCACGGCTGGAGTCCAGACGCCAGTAGTGGGAGCCAGTGAAGGCATAGGTGGCACCGCGATGGTCAGACAGCAGTGCAGTCAGGCC
+
#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-F-FFFFFFFFFFFFFF--FFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.2 2/2
NATCAAGGCCATGCGGATCCTGGCCTCAGGTGACAACCAGGGCATCCCCATCCTGTCCAACATAAAGCTGAAAGAGGAGCAGCGCATAACCACGACTTCC
+
#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.3 3/2
NCCGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTA
+
#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF8FFF
@SRR6575753.4 4/2
GTGTCCTGGGGGTCAAAGGATATCTTCCATTTGCCTTTAAAGTAGATGTAATTCACCAGCACCATCAATGTCCCATCATCCAGTTCTGAGATCAGTTTCT
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR6575753.5 5/2
ACGCAGAGTACATGGGAGAGCAGCCGCGGCTCAGCGAGGCCGGCCGCCGGGTACTTCCTGTGGAGGCCGCAGCAGGCGCGAGCGCCCAGGACCTGCCAGC
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF-FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Starting FASTQC command


Processing SRR6575753_1.fastq

Printing command:
fastqc --outdir SRR6575753_1.fastq

Analysis complete for SRR6575753_1.fastq

Processing SRR6575753_2.fastq

Printing command:
fastqc --outdir SRR6575753_2.fastq

Analysis complete for SRR6575753_2.fastq

Finished FASTQC command


Copy output to storage dir


List files in scratch

total 365M
drwx------   3 kkarri waxmanlab 4.0K Oct 12 19:42 .
drwxrwxrwt. 89 root   root      108K Oct 12 19:42 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 19:42 B3-MAA100138-3_56_F-1-1_FASTQC
-rw-r--r--   1 kkarri waxmanlab 182M Oct 12 19:42 SRR6575753_1.fastq
-rw-r--r--   1 kkarri waxmanlab 182M Oct 12 19:42 SRR6575753_2.fastq
==========================================================
Finished on : Fri Oct 12 19:43:00 EDT 2018
0 hours, 0 minutes and 39 seconds elapsed.
==========================================================