[2018-10-13 03:26:30] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:26:30] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:26:30] Checking for Bowtie index files (genome).. [2018-10-13 03:26:30] Checking for reference FASTA file [2018-10-13 03:26:30] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:26:34] Reading known junctions from GTF file [2018-10-13 03:26:38] Preparing reads left reads: min. length=100, max. length=100, 372455 kept reads (271 discarded) right reads: min. length=100, max. length=100, 372210 kept reads (516 discarded) [2018-10-13 03:26:55] Building transcriptome data files /scratch/8792957.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:27:14] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:36:00] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:36:40] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:37:19] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:37:19] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:38:01] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:38:10] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:38:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:38:33] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:38:43] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:39:26] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:39:36] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:39:49] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:40:00] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:40:10] Searching for junctions via segment mapping [2018-10-13 03:42:53] Retrieving sequences for splices [2018-10-13 03:45:02] Indexing splices [2018-10-13 03:45:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:45:28] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:45:32] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:45:37] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:45:41] Joining segment hits [2018-10-13 03:48:17] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:48:21] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:48:26] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:48:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:48:35] Joining segment hits [2018-10-13 03:51:05] Reporting output tracks ----------------------------------------------- [2018-10-13 03:54:39] A summary of the alignment counts can be found in /scratch/8792957.1.linga/tophat2/align_summary.txt [2018-10-13 03:54:39] Run complete: 00:28:09 elapsed