-----------------------
Start of variable list:
-----------------------
Sample_ID:
F17-MAA000907-3_11_M-1-1
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/00_Setup_Pipeline
Bowtie2Index_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/Bowtie2
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/GTF_Files
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
STRANDEDNESS:
fr-unstranded
DISTANCE_BT_READ_PAIR:
-41
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Sat Oct 13 18:12:55 EDT 2018
Running on node : scc-ka6
Current directory : /restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/04_TopHat_Paired_End
Current job ID : 8793429
Current job name : Step_04_F17-MAA000907-3_11_M-1-1
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/8793429.1.linga

Loading required modules...


Here is the Left_List:
SRR6576339_1.fastq.gz


Here is the Right_List:
SRR6576339_2.fastq.gz


List files in the scratch directory:

total 105M
drwx------    4 kkarri waxmanlab 4.0K Oct 13 18:13 .
drwxrwxrwt. 107 root   root       92K Oct 13 18:12 ..
drwxr-xr-x    2 kkarri waxmanlab 4.0K Oct 13 18:13 Bowtie2Index
-rw-r--r--    1 kkarri waxmanlab  72M Oct 13 18:13 RefSeq_GeneBody.gtf
-rw-r--r--    1 kkarri waxmanlab  16M Oct 13 18:12 SRR6576339_1.fastq.gz
-rw-r--r--    1 kkarri waxmanlab  17M Oct 13 18:12 SRR6576339_2.fastq.gz
drwxr-xr-x    2 kkarri waxmanlab 4.0K Oct 13 18:13 tophat2

Starting to run my commands


Starting tophat

tophat --no-coverage-search -o /scratch/8793429.1.linga/tophat2 -G RefSeq_GeneBody.gtf -p 16 --library-type fr-unstranded -r -41 --mate-std-dev 150 Bowtie2Index/genome SRR6576339_1.fastq.gz SRR6576339_2.fastq.gz

Ending tophat


Starting samtools commands


Ending samtools commands


Starting spliced_read_counts


Ending spliced_read_counts


List files in scratch

total 105M
drwx------    4 kkarri waxmanlab 4.0K Oct 13 18:40 .
drwxrwxrwt. 104 root   root       92K Oct 13 18:35 ..
drwxr-xr-x    2 kkarri waxmanlab 4.0K Oct 13 18:13 Bowtie2Index
-rw-r--r--    1 kkarri waxmanlab  72M Oct 13 18:13 RefSeq_GeneBody.gtf
-rw-r--r--    1 kkarri waxmanlab  16M Oct 13 18:12 SRR6576339_1.fastq.gz
-rw-r--r--    1 kkarri waxmanlab  17M Oct 13 18:12 SRR6576339_2.fastq.gz
-rw-r--r--    1 kkarri waxmanlab    7 Oct 13 18:40 numunique_hits.txt
drwxr-xr-x    3 kkarri waxmanlab 4.0K Oct 13 18:41 tophat2
==========================================================
Finished on : Sat Oct 13 18:41:02 EDT 2018
0 hours, 28 minutes and 7 seconds elapsed.
==========================================================