-----------------------
Start of variable list:
-----------------------
Sample_ID:
E3-MAA100042-3_9_M-1-1
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/00_Setup_Pipeline
Bowtie2Index_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/Bowtie2
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/tabula_muris/GTF_Files
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
STRANDEDNESS:
fr-unstranded
DISTANCE_BT_READ_PAIR:
-41
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Fri Oct 12 23:03:20 EDT 2018
Running on node : scc-pf3
Current directory : /restricted/projectnb/waxmanlab/kkarri/tabula_muris/smartseq_fastq/Scripts/04_TopHat_Paired_End
Current job ID : 8792819
Current job name : Step_04_E3-MAA100042-3_9_M-1-1
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/8792819.1.p8

Loading required modules...


Here is the Left_List:
SRR6575624_1.fastq.gz


Here is the Right_List:
SRR6575624_2.fastq.gz


List files in the scratch directory:

total 328M
drwx------   4 kkarri waxmanlab 4.0K Oct 12 23:03 .
drwxrwxrwt. 23 root   root       16K Oct 12 23:03 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 23:03 Bowtie2Index
-rw-r--r--   1 kkarri waxmanlab  72M Oct 12 23:03 RefSeq_GeneBody.gtf
-rw-r--r--   1 kkarri waxmanlab 125M Oct 12 23:03 SRR6575624_1.fastq.gz
-rw-r--r--   1 kkarri waxmanlab 132M Oct 12 23:03 SRR6575624_2.fastq.gz
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 23:03 tophat2

Starting to run my commands


Starting tophat

tophat --no-coverage-search -o /scratch/8792819.1.p8/tophat2 -G RefSeq_GeneBody.gtf -p 16 --library-type fr-unstranded -r -41 --mate-std-dev 150 Bowtie2Index/genome SRR6575624_1.fastq.gz SRR6575624_2.fastq.gz

Ending tophat


Starting samtools commands


Ending samtools commands


Starting spliced_read_counts


Ending spliced_read_counts


List files in scratch

total 328M
drwx------   4 kkarri waxmanlab 4.0K Oct 12 23:52 .
drwxrwxrwt. 23 root   root       16K Oct 12 23:03 ..
drwxr-xr-x   2 kkarri waxmanlab 4.0K Oct 12 23:03 Bowtie2Index
-rw-r--r--   1 kkarri waxmanlab  72M Oct 12 23:03 RefSeq_GeneBody.gtf
-rw-r--r--   1 kkarri waxmanlab 125M Oct 12 23:03 SRR6575624_1.fastq.gz
-rw-r--r--   1 kkarri waxmanlab 132M Oct 12 23:03 SRR6575624_2.fastq.gz
-rw-r--r--   1 kkarri waxmanlab    8 Oct 12 23:52 numunique_hits.txt
drwxr-xr-x   3 kkarri waxmanlab 4.0K Oct 12 23:53 tophat2
==========================================================
Finished on : Fri Oct 12 23:53:55 EDT 2018
0 hours, 50 minutes and 35 seconds elapsed.
==========================================================