[2018-10-13 07:27:23] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:27:23] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:27:23] Checking for Bowtie index files (genome).. [2018-10-13 07:27:23] Checking for reference FASTA file [2018-10-13 07:27:23] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:27:25] Reading known junctions from GTF file [2018-10-13 07:27:28] Preparing reads left reads: min. length=100, max. length=100, 1241621 kept reads (185 discarded) right reads: min. length=100, max. length=100, 1241235 kept reads (571 discarded) [2018-10-13 07:28:00] Building transcriptome data files /scratch/8793088.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:28:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:32:59] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:34:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:35:21] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:35:21] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:35:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:35:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:35:58] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:36:05] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:36:12] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:36:37] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:36:43] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:36:52] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:36:59] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:37:08] Searching for junctions via segment mapping [2018-10-13 07:42:27] Retrieving sequences for splices [2018-10-13 07:43:34] Indexing splices Building a SMALL index [2018-10-13 07:43:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:43:51] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:43:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:44:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:44:09] Joining segment hits [2018-10-13 07:45:26] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:45:30] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:45:37] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:45:43] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:45:49] Joining segment hits [2018-10-13 07:47:07] Reporting output tracks ----------------------------------------------- [2018-10-13 08:05:02] A summary of the alignment counts can be found in /scratch/8793088.1.p8/tophat2/align_summary.txt [2018-10-13 08:05:02] Run complete: 00:37:38 elapsed [samopen] SAM header is present: 114 sequences.