[2018-10-13 03:22:51] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:22:51] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:22:51] Checking for Bowtie index files (genome).. [2018-10-13 03:22:51] Checking for reference FASTA file [2018-10-13 03:22:51] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:22:53] Reading known junctions from GTF file [2018-10-13 03:22:56] Preparing reads left reads: min. length=100, max. length=100, 246811 kept reads (234 discarded) right reads: min. length=100, max. length=100, 246540 kept reads (505 discarded) [2018-10-13 03:23:03] Building transcriptome data files /scratch/8792956.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:23:13] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:29:02] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:29:22] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:29:43] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:29:43] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:30:02] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:30:06] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:30:12] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:30:17] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:30:22] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:30:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:30:46] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:30:52] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:30:57] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:31:02] Searching for junctions via segment mapping [2018-10-13 03:32:23] Retrieving sequences for splices [2018-10-13 03:33:36] Indexing splices [2018-10-13 03:33:46] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:33:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:33:51] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:33:53] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:33:55] Joining segment hits [2018-10-13 03:35:11] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:35:13] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:35:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:35:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:35:20] Joining segment hits [2018-10-13 03:36:36] Reporting output tracks ----------------------------------------------- [2018-10-13 03:38:10] A summary of the alignment counts can be found in /scratch/8792956.1.c/tophat2/align_summary.txt [2018-10-13 03:38:10] Run complete: 00:15:18 elapsed