[2018-10-13 03:14:52] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:14:52] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:14:52] Checking for Bowtie index files (genome).. [2018-10-13 03:14:52] Checking for reference FASTA file [2018-10-13 03:14:52] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:14:54] Reading known junctions from GTF file [2018-10-13 03:14:57] Preparing reads left reads: min. length=100, max. length=100, 300756 kept reads (336 discarded) right reads: min. length=100, max. length=100, 300537 kept reads (555 discarded) [2018-10-13 03:15:05] Building transcriptome data files /scratch/8792949.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:15:15] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:20:05] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:20:28] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:20:52] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:20:52] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:21:16] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:21:20] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:21:26] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:21:32] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:21:37] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:22:01] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:22:06] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:22:12] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:22:17] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:22:23] Searching for junctions via segment mapping [2018-10-13 03:23:37] Retrieving sequences for splices [2018-10-13 03:24:44] Indexing splices [2018-10-13 03:24:55] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:24:57] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:25:00] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:25:02] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:25:04] Joining segment hits [2018-10-13 03:26:14] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:26:17] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:26:19] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:26:21] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:26:23] Joining segment hits [2018-10-13 03:27:34] Reporting output tracks ----------------------------------------------- [2018-10-13 03:29:07] A summary of the alignment counts can be found in /scratch/8792949.1.p8/tophat2/align_summary.txt [2018-10-13 03:29:07] Run complete: 00:14:15 elapsed