[2018-10-13 16:30:10] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:30:10] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:30:10] Checking for Bowtie index files (genome).. [2018-10-13 16:30:10] Checking for reference FASTA file [2018-10-13 16:30:10] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:30:15] Reading known junctions from GTF file [2018-10-13 16:30:21] Preparing reads left reads: min. length=100, max. length=100, 38401 kept reads (54 discarded) right reads: min. length=100, max. length=100, 38394 kept reads (61 discarded) [2018-10-13 16:30:23] Building transcriptome data files /scratch/8793358.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:30:51] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:41:07] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:41:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:41:24] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:41:24] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:41:33] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:41:40] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:41:47] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:41:55] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:42:02] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:42:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:42:19] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:42:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:42:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:42:40] Searching for junctions via segment mapping [2018-10-13 16:44:55] Retrieving sequences for splices [2018-10-13 16:47:00] Indexing splices [2018-10-13 16:47:18] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:47:22] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:47:26] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:47:30] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:47:34] Joining segment hits [2018-10-13 16:49:45] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:49:49] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:49:52] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:49:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:50:00] Joining segment hits [2018-10-13 16:52:06] Reporting output tracks ----------------------------------------------- [2018-10-13 16:54:26] A summary of the alignment counts can be found in /scratch/8793358.1.linga/tophat2/align_summary.txt [2018-10-13 16:54:26] Run complete: 00:24:15 elapsed