[2018-10-13 16:28:40] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:28:40] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:28:40] Checking for Bowtie index files (genome).. [2018-10-13 16:28:40] Checking for reference FASTA file [2018-10-13 16:28:40] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:28:46] Reading known junctions from GTF file [2018-10-13 16:28:50] Preparing reads left reads: min. length=100, max. length=100, 349447 kept reads (467 discarded) right reads: min. length=100, max. length=100, 349184 kept reads (730 discarded) [2018-10-13 16:29:06] Building transcriptome data files /scratch/8793357.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:29:27] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:37:37] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:38:21] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:39:06] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:39:06] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:39:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:39:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:40:06] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:40:17] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:40:27] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:41:04] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:41:14] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:41:30] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:41:42] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:41:52] Searching for junctions via segment mapping [2018-10-13 16:45:21] Retrieving sequences for splices [2018-10-13 16:47:34] Indexing splices [2018-10-13 16:47:58] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:48:03] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:48:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:48:15] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:48:20] Joining segment hits [2018-10-13 16:51:02] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:51:07] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:51:13] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:51:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:51:24] Joining segment hits [2018-10-13 16:53:48] Reporting output tracks ----------------------------------------------- [2018-10-13 16:59:00] A summary of the alignment counts can be found in /scratch/8793357.1.linga/tophat2/align_summary.txt [2018-10-13 16:59:00] Run complete: 00:30:19 elapsed