[2018-10-13 03:17:02] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:17:02] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:17:03] Checking for Bowtie index files (genome).. [2018-10-13 03:17:03] Checking for reference FASTA file [2018-10-13 03:17:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:17:07] Reading known junctions from GTF file [2018-10-13 03:17:12] Preparing reads left reads: min. length=100, max. length=100, 386051 kept reads (326 discarded) right reads: min. length=100, max. length=100, 385715 kept reads (662 discarded) [2018-10-13 03:17:27] Building transcriptome data files /scratch/8792954.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:17:47] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:25:40] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:26:20] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:27:01] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:27:01] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:27:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:27:54] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:28:08] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:28:18] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:28:29] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:29:13] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:29:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:29:38] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:29:50] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:30:01] Searching for junctions via segment mapping [2018-10-13 03:32:41] Retrieving sequences for splices [2018-10-13 03:34:47] Indexing splices [2018-10-13 03:35:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:35:10] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:35:15] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:35:19] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:35:23] Joining segment hits [2018-10-13 03:37:33] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:37:37] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:37:42] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:37:46] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:37:50] Joining segment hits [2018-10-13 03:40:00] Reporting output tracks ----------------------------------------------- [2018-10-13 03:43:21] A summary of the alignment counts can be found in /scratch/8792954.1.linga/tophat2/align_summary.txt [2018-10-13 03:43:21] Run complete: 00:26:18 elapsed