[2018-10-13 03:06:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:06:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:06:25] Checking for Bowtie index files (genome).. [2018-10-13 03:06:25] Checking for reference FASTA file [2018-10-13 03:06:25] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:06:27] Reading known junctions from GTF file [2018-10-13 03:06:30] Preparing reads left reads: min. length=100, max. length=100, 54037 kept reads (83 discarded) right reads: min. length=100, max. length=100, 54025 kept reads (95 discarded) [2018-10-13 03:06:31] Building transcriptome data files /scratch/8792942.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:06:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:12:31] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:12:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:12:44] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:12:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:12:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:12:54] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:12:58] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:13:01] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:13:05] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:13:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:13:15] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:13:19] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:13:23] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:13:26] Searching for junctions via segment mapping [2018-10-13 03:14:39] Retrieving sequences for splices [2018-10-13 03:15:52] Indexing splices [2018-10-13 03:16:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:16:05] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:16:07] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:16:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:16:11] Joining segment hits [2018-10-13 03:17:24] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:17:26] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:17:29] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:17:31] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:17:33] Joining segment hits [2018-10-13 03:18:47] Reporting output tracks ----------------------------------------------- [2018-10-13 03:20:02] A summary of the alignment counts can be found in /scratch/8792942.1.c/tophat2/align_summary.txt [2018-10-13 03:20:02] Run complete: 00:13:37 elapsed