[2018-10-13 03:00:26] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 03:00:26] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 03:00:26] Checking for Bowtie index files (genome).. [2018-10-13 03:00:26] Checking for reference FASTA file [2018-10-13 03:00:26] Generating SAM header for Bowtie2Index/genome [2018-10-13 03:00:31] Reading known junctions from GTF file [2018-10-13 03:00:35] Preparing reads left reads: min. length=100, max. length=100, 671814 kept reads (429 discarded) right reads: min. length=100, max. length=100, 671376 kept reads (867 discarded) [2018-10-13 03:01:04] Building transcriptome data files /scratch/8792940.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 03:01:23] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 03:09:18] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:10:13] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:11:09] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:11:09] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:12:04] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:12:15] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:12:31] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:12:42] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:12:54] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:13:52] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:14:04] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:14:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:14:35] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:14:47] Searching for junctions via segment mapping [2018-10-13 03:17:48] Retrieving sequences for splices [2018-10-13 03:20:01] Indexing splices [2018-10-13 03:20:22] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:20:26] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:20:32] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:20:37] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:20:41] Joining segment hits [2018-10-13 03:23:14] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:23:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:23:25] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:23:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:23:35] Joining segment hits [2018-10-13 03:26:07] Reporting output tracks ----------------------------------------------- [2018-10-13 03:30:57] A summary of the alignment counts can be found in /scratch/8792940.1.linga/tophat2/align_summary.txt [2018-10-13 03:30:57] Run complete: 00:30:30 elapsed