[2018-10-13 02:54:08] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:54:08] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:54:08] Checking for Bowtie index files (genome).. [2018-10-13 02:54:08] Checking for reference FASTA file [2018-10-13 02:54:08] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:54:11] Reading known junctions from GTF file [2018-10-13 02:54:13] Preparing reads left reads: min. length=100, max. length=100, 374851 kept reads (199 discarded) right reads: min. length=100, max. length=100, 374576 kept reads (474 discarded) [2018-10-13 02:54:24] Building transcriptome data files /scratch/8792932.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:54:35] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:59:17] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:59:38] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 03:00:01] Resuming TopHat pipeline with unmapped reads [2018-10-13 03:00:01] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:00:22] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:00:27] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:00:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:00:37] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:00:42] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:01:04] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:01:09] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:01:15] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:01:20] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:01:25] Searching for junctions via segment mapping [2018-10-13 03:02:44] Retrieving sequences for splices [2018-10-13 03:03:51] Indexing splices [2018-10-13 03:04:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:04:04] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:04:06] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:04:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:04:11] Joining segment hits [2018-10-13 03:05:21] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:05:23] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:05:25] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:05:27] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:05:29] Joining segment hits [2018-10-13 03:06:39] Reporting output tracks ----------------------------------------------- [2018-10-13 03:08:25] A summary of the alignment counts can be found in /scratch/8792932.1.p16/tophat2/align_summary.txt [2018-10-13 03:08:25] Run complete: 00:14:16 elapsed