[2018-10-13 16:24:47] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:24:47] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:24:47] Checking for Bowtie index files (genome).. [2018-10-13 16:24:47] Checking for reference FASTA file [2018-10-13 16:24:47] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:24:49] Reading known junctions from GTF file [2018-10-13 16:24:51] Preparing reads left reads: min. length=100, max. length=100, 419548 kept reads (288 discarded) right reads: min. length=100, max. length=100, 419281 kept reads (555 discarded) [2018-10-13 16:25:04] Building transcriptome data files /scratch/8793353.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:25:14] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:30:02] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:30:23] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:30:45] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:30:45] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:30:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:31:03] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:31:08] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:31:12] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:31:17] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:31:31] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:31:35] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:31:41] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:31:46] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:31:51] Searching for junctions via segment mapping [2018-10-13 16:33:29] Retrieving sequences for splices [2018-10-13 16:34:36] Indexing splices [2018-10-13 16:34:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:34:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:34:52] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:34:54] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:34:57] Joining segment hits [2018-10-13 16:36:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:36:12] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:36:15] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:36:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:36:20] Joining segment hits [2018-10-13 16:37:34] Reporting output tracks ----------------------------------------------- [2018-10-13 16:39:56] A summary of the alignment counts can be found in /scratch/8793353.1.p8/tophat2/align_summary.txt [2018-10-13 16:39:56] Run complete: 00:15:09 elapsed