[2018-10-13 02:50:34] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:50:34] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:50:34] Checking for Bowtie index files (genome).. [2018-10-13 02:50:34] Checking for reference FASTA file [2018-10-13 02:50:34] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:50:38] Reading known junctions from GTF file [2018-10-13 02:50:42] Preparing reads left reads: min. length=100, max. length=100, 48851 kept reads (64 discarded) right reads: min. length=100, max. length=100, 48859 kept reads (56 discarded) [2018-10-13 02:50:44] Building transcriptome data files /scratch/8792931.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:51:04] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:59:17] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:59:28] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:59:39] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:59:39] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:59:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:59:58] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:00:05] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:00:13] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:00:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 03:00:33] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 03:00:40] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 03:00:47] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 03:00:55] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 03:01:03] Searching for junctions via segment mapping [2018-10-13 03:03:13] Retrieving sequences for splices [2018-10-13 03:05:24] Indexing splices [2018-10-13 03:05:45] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:05:48] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:05:52] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:05:57] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:06:01] Joining segment hits [2018-10-13 03:08:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:08:14] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:08:18] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:08:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:08:25] Joining segment hits [2018-10-13 03:10:36] Reporting output tracks ----------------------------------------------- [2018-10-13 03:12:54] A summary of the alignment counts can be found in /scratch/8792931.1.linga/tophat2/align_summary.txt [2018-10-13 03:12:54] Run complete: 00:22:20 elapsed