[2018-10-13 18:38:53] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:38:53] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:38:53] Checking for Bowtie index files (genome).. [2018-10-13 18:38:53] Checking for reference FASTA file [2018-10-13 18:38:53] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:38:55] Reading known junctions from GTF file [2018-10-13 18:38:57] Preparing reads left reads: min. length=100, max. length=100, 419866 kept reads (258 discarded) right reads: min. length=100, max. length=100, 419599 kept reads (525 discarded) [2018-10-13 18:39:09] Building transcriptome data files /scratch/8793449.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:39:19] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:44:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:44:57] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:45:18] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:45:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:45:35] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:45:39] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:45:44] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:45:49] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:45:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:46:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:46:16] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:46:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:46:27] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:46:32] Searching for junctions via segment mapping [2018-10-13 18:47:54] Retrieving sequences for splices [2018-10-13 18:49:01] Indexing splices [2018-10-13 18:49:11] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:49:14] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:49:16] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:49:18] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:49:21] Joining segment hits [2018-10-13 18:50:35] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:50:38] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:50:40] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:50:42] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:50:45] Joining segment hits [2018-10-13 18:51:59] Reporting output tracks ----------------------------------------------- [2018-10-13 18:53:46] A summary of the alignment counts can be found in /scratch/8793449.1.p8/tophat2/align_summary.txt [2018-10-13 18:53:46] Run complete: 00:14:53 elapsed