[2018-10-13 16:17:55] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:17:55] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:17:55] Checking for Bowtie index files (genome).. [2018-10-13 16:17:55] Checking for reference FASTA file [2018-10-13 16:17:55] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:18:01] Reading known junctions from GTF file [2018-10-13 16:18:06] Preparing reads left reads: min. length=100, max. length=100, 149238 kept reads (169 discarded) right reads: min. length=100, max. length=100, 149224 kept reads (183 discarded) [2018-10-13 16:18:14] Building transcriptome data files /scratch/8793350.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:18:38] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:29:31] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:29:50] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:30:09] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:30:09] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:30:24] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:30:31] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:30:41] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:30:48] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:30:55] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:31:15] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:31:24] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:31:38] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:31:46] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:31:58] Searching for junctions via segment mapping [2018-10-13 16:35:54] Retrieving sequences for splices [2018-10-13 16:38:12] Indexing splices [2018-10-13 16:38:32] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:38:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:38:43] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:38:49] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:38:55] Joining segment hits [2018-10-13 16:42:00] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:42:05] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:42:14] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:42:19] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:42:26] Joining segment hits [2018-10-13 16:45:09] Reporting output tracks ----------------------------------------------- [2018-10-13 16:49:03] A summary of the alignment counts can be found in /scratch/8793350.1.linga/tophat2/align_summary.txt [2018-10-13 16:49:03] Run complete: 00:31:08 elapsed