[2018-10-13 02:43:20] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:43:20] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:43:20] Checking for Bowtie index files (genome).. [2018-10-13 02:43:20] Checking for reference FASTA file [2018-10-13 02:43:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:43:22] Reading known junctions from GTF file [2018-10-13 02:43:24] Preparing reads left reads: min. length=100, max. length=100, 239362 kept reads (137 discarded) right reads: min. length=100, max. length=100, 239172 kept reads (327 discarded) [2018-10-13 02:43:32] Building transcriptome data files /scratch/8792927.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:43:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:48:30] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:48:46] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:49:02] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:49:02] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:49:17] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:49:21] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:49:26] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:49:31] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:49:39] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:49:56] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:50:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:50:06] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:50:11] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:50:15] Searching for junctions via segment mapping [2018-10-13 02:51:35] Retrieving sequences for splices [2018-10-13 02:52:42] Indexing splices [2018-10-13 02:52:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:52:54] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:52:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:52:59] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:53:01] Joining segment hits [2018-10-13 02:54:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:54:12] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:54:14] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:54:17] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:54:19] Joining segment hits [2018-10-13 02:55:28] Reporting output tracks ----------------------------------------------- [2018-10-13 02:57:02] A summary of the alignment counts can be found in /scratch/8792927.1.p8/tophat2/align_summary.txt [2018-10-13 02:57:02] Run complete: 00:13:41 elapsed