[2018-10-13 02:41:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:41:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:41:03] Checking for Bowtie index files (genome).. [2018-10-13 02:41:03] Checking for reference FASTA file [2018-10-13 02:41:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:41:07] Reading known junctions from GTF file [2018-10-13 02:41:12] Preparing reads left reads: min. length=100, max. length=100, 511136 kept reads (427 discarded) right reads: min. length=100, max. length=100, 511140 kept reads (423 discarded) [2018-10-13 02:41:37] Building transcriptome data files /scratch/8792923.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:41:56] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:50:30] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:51:13] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:51:57] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:51:57] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:52:50] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:53:01] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:53:14] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:53:24] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:53:34] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:54:26] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:54:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:54:52] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:55:03] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:55:14] Searching for junctions via segment mapping [2018-10-13 02:57:57] Retrieving sequences for splices [2018-10-13 03:00:22] Indexing splices [2018-10-13 03:00:43] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:00:48] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:00:53] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:00:57] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:01:01] Joining segment hits [2018-10-13 03:03:34] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 03:03:38] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 03:03:43] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 03:03:48] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 03:03:52] Joining segment hits [2018-10-13 03:06:28] Reporting output tracks ----------------------------------------------- [2018-10-13 03:10:24] A summary of the alignment counts can be found in /scratch/8792923.1.linga/tophat2/align_summary.txt [2018-10-13 03:10:24] Run complete: 00:29:21 elapsed