[2018-10-13 16:12:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:12:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:12:03] Checking for Bowtie index files (genome).. [2018-10-13 16:12:03] Checking for reference FASTA file [2018-10-13 16:12:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:12:05] Reading known junctions from GTF file [2018-10-13 16:12:07] Preparing reads left reads: min. length=100, max. length=100, 75876 kept reads (189 discarded) right reads: min. length=100, max. length=100, 75778 kept reads (287 discarded) [2018-10-13 16:12:09] Building transcriptome data files /scratch/8793347.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:12:19] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:17:01] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:17:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:17:18] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:17:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:17:27] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:17:30] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:17:35] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:17:38] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:17:42] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:17:51] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:17:54] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:17:58] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:18:02] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:18:06] Searching for junctions via segment mapping [2018-10-13 16:19:13] Retrieving sequences for splices [2018-10-13 16:20:20] Indexing splices [2018-10-13 16:20:31] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:20:32] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:20:35] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:20:37] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:20:39] Joining segment hits [2018-10-13 16:21:47] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:21:49] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:21:51] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:21:53] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:21:55] Joining segment hits [2018-10-13 16:23:04] Reporting output tracks ----------------------------------------------- [2018-10-13 16:24:14] A summary of the alignment counts can be found in /scratch/8793347.1.p16/tophat2/align_summary.txt [2018-10-13 16:24:14] Run complete: 00:12:11 elapsed