[2018-10-12 21:30:59] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:30:59] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:30:59] Checking for Bowtie index files (genome).. [2018-10-12 21:30:59] Checking for reference FASTA file [2018-10-12 21:30:59] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:31:04] Reading known junctions from GTF file [2018-10-12 21:31:09] Preparing reads left reads: min. length=100, max. length=100, 311851 kept reads (264 discarded) right reads: min. length=100, max. length=100, 311859 kept reads (256 discarded) [2018-10-12 21:31:24] Building transcriptome data files /scratch/8792759.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:31:44] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:40:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:41:25] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:41:57] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:41:58] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:42:34] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:42:43] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:42:56] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:43:06] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:43:16] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:43:52] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:44:03] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:44:16] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:44:27] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:44:38] Searching for junctions via segment mapping [2018-10-12 21:47:21] Retrieving sequences for splices [2018-10-12 21:49:32] Indexing splices [2018-10-12 21:49:55] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:49:59] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:50:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:50:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:50:14] Joining segment hits [2018-10-12 21:52:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:52:49] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:52:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:52:58] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:53:03] Joining segment hits [2018-10-12 21:55:48] Reporting output tracks ----------------------------------------------- [2018-10-12 21:59:12] A summary of the alignment counts can be found in /scratch/8792759.1.linga/tophat2/align_summary.txt [2018-10-12 21:59:12] Run complete: 00:28:13 elapsed