[2018-10-13 16:12:09] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:12:09] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:12:09] Checking for Bowtie index files (genome).. [2018-10-13 16:12:09] Checking for reference FASTA file [2018-10-13 16:12:09] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:12:13] Reading known junctions from GTF file [2018-10-13 16:12:18] Preparing reads left reads: min. length=100, max. length=100, 410017 kept reads (229 discarded) right reads: min. length=100, max. length=100, 409642 kept reads (604 discarded) [2018-10-13 16:12:36] Building transcriptome data files /scratch/8793346.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:12:55] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:20:45] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:21:24] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:22:04] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:22:04] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:22:28] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:22:37] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:22:49] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:22:58] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:23:08] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:23:35] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:23:45] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:23:58] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:24:09] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:24:20] Searching for junctions via segment mapping [2018-10-13 16:28:25] Retrieving sequences for splices [2018-10-13 16:30:36] Indexing splices [2018-10-13 16:30:57] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:31:01] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:31:08] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:31:13] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:31:19] Joining segment hits [2018-10-13 16:33:45] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:33:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:33:56] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:34:02] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:34:08] Joining segment hits [2018-10-13 16:36:32] Reporting output tracks ----------------------------------------------- [2018-10-13 16:45:04] A summary of the alignment counts can be found in /scratch/8793346.1.linga/tophat2/align_summary.txt [2018-10-13 16:45:04] Run complete: 00:32:55 elapsed