[2018-10-13 17:35:59] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:35:59] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:35:59] Checking for Bowtie index files (genome).. [2018-10-13 17:35:59] Checking for reference FASTA file [2018-10-13 17:35:59] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:36:04] Reading known junctions from GTF file [2018-10-13 17:36:08] Preparing reads left reads: min. length=100, max. length=100, 114293 kept reads (185 discarded) right reads: min. length=100, max. length=100, 114180 kept reads (298 discarded) [2018-10-13 17:36:14] Building transcriptome data files /scratch/8793405.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:36:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:44:39] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:45:00] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:45:20] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:45:20] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:45:39] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:45:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:45:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:46:05] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:46:14] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:46:33] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:46:42] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:46:52] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:47:01] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:47:10] Searching for junctions via segment mapping [2018-10-13 17:49:32] Retrieving sequences for splices [2018-10-13 17:51:47] Indexing splices [2018-10-13 17:52:04] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:52:08] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:52:13] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:52:17] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:52:21] Joining segment hits [2018-10-13 17:54:50] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:54:54] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:54:58] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:55:02] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:55:07] Joining segment hits [2018-10-13 17:57:26] Reporting output tracks ----------------------------------------------- [2018-10-13 17:59:56] A summary of the alignment counts can be found in /scratch/8793405.1.linga/tophat2/align_summary.txt [2018-10-13 17:59:56] Run complete: 00:23:57 elapsed