[2018-10-13 02:19:49] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:19:49] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:19:49] Checking for Bowtie index files (genome).. [2018-10-13 02:19:49] Checking for reference FASTA file [2018-10-13 02:19:49] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:19:53] Reading known junctions from GTF file [2018-10-13 02:19:58] Preparing reads left reads: min. length=100, max. length=100, 409239 kept reads (291 discarded) right reads: min. length=100, max. length=100, 408910 kept reads (620 discarded) [2018-10-13 02:20:16] Building transcriptome data files /scratch/8792911.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:20:35] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:29:03] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:29:44] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:30:25] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:30:25] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:31:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:31:19] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:31:31] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:31:41] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:31:51] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:32:32] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:32:42] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:32:55] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:33:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:33:14] Searching for junctions via segment mapping [2018-10-13 02:36:01] Retrieving sequences for splices [2018-10-13 02:38:15] Indexing splices [2018-10-13 02:38:34] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:38:38] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:38:43] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:38:47] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:38:51] Joining segment hits [2018-10-13 02:41:08] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:41:12] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:41:17] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:41:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:41:26] Joining segment hits [2018-10-13 02:44:05] Reporting output tracks ----------------------------------------------- [2018-10-13 02:48:00] A summary of the alignment counts can be found in /scratch/8792911.1.linga/tophat2/align_summary.txt [2018-10-13 02:48:00] Run complete: 00:28:11 elapsed