[2018-10-13 18:35:16] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:35:16] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:35:16] Checking for Bowtie index files (genome).. [2018-10-13 18:35:16] Checking for reference FASTA file [2018-10-13 18:35:16] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:35:18] Reading known junctions from GTF file [2018-10-13 18:35:21] Preparing reads left reads: min. length=100, max. length=100, 322962 kept reads (172 discarded) right reads: min. length=100, max. length=100, 322736 kept reads (398 discarded) [2018-10-13 18:35:29] Building transcriptome data files /scratch/8793445.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:35:39] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:40:22] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:40:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:40:54] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:40:54] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:41:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:41:12] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:41:17] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:41:21] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:41:25] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:41:40] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:41:44] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:41:49] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:41:54] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:41:58] Searching for junctions via segment mapping [2018-10-13 18:43:17] Retrieving sequences for splices [2018-10-13 18:44:24] Indexing splices [2018-10-13 18:44:34] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:44:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:44:39] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:44:41] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:44:43] Joining segment hits [2018-10-13 18:45:52] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:45:54] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:45:57] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:45:59] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:46:02] Joining segment hits [2018-10-13 18:47:11] Reporting output tracks ----------------------------------------------- [2018-10-13 18:48:45] A summary of the alignment counts can be found in /scratch/8793445.1.p16/tophat2/align_summary.txt [2018-10-13 18:48:45] Run complete: 00:13:29 elapsed