[2018-10-13 02:12:00] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 02:12:00] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 02:12:00] Checking for Bowtie index files (genome).. [2018-10-13 02:12:00] Checking for reference FASTA file [2018-10-13 02:12:00] Generating SAM header for Bowtie2Index/genome [2018-10-13 02:12:05] Reading known junctions from GTF file [2018-10-13 02:12:09] Preparing reads left reads: min. length=100, max. length=100, 115861 kept reads (160 discarded) right reads: min. length=100, max. length=100, 115770 kept reads (251 discarded) [2018-10-13 02:12:14] Building transcriptome data files /scratch/8792908.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:12:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:20:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:20:57] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:21:16] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:21:17] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:21:39] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:21:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:21:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:22:06] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:22:14] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:22:37] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:22:46] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:22:56] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:23:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:23:14] Searching for junctions via segment mapping [2018-10-13 02:25:28] Retrieving sequences for splices [2018-10-13 02:27:33] Indexing splices [2018-10-13 02:27:56] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:28:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:28:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:28:08] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:28:12] Joining segment hits [2018-10-13 02:30:21] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:30:25] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:30:30] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:30:34] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:30:38] Joining segment hits [2018-10-13 02:33:07] Reporting output tracks ----------------------------------------------- [2018-10-13 02:35:47] A summary of the alignment counts can be found in /scratch/8792908.1.linga/tophat2/align_summary.txt [2018-10-13 02:35:47] Run complete: 00:23:47 elapsed