[2018-10-13 16:00:47] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:00:47] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:00:47] Checking for Bowtie index files (genome).. [2018-10-13 16:00:47] Checking for reference FASTA file [2018-10-13 16:00:47] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:00:53] Reading known junctions from GTF file [2018-10-13 16:00:59] Preparing reads left reads: min. length=100, max. length=100, 150538 kept reads (143 discarded) right reads: min. length=100, max. length=100, 150444 kept reads (237 discarded) [2018-10-13 16:01:07] Building transcriptome data files /scratch/8793342.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 16:01:34] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:11:09] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:11:32] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:11:56] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:11:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:12:26] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:12:34] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:12:45] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:12:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:13:02] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:13:29] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:13:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:13:48] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:13:57] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:14:06] Searching for junctions via segment mapping [2018-10-13 16:17:14] Retrieving sequences for splices [2018-10-13 16:20:06] Indexing splices [2018-10-13 16:20:31] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:20:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:20:40] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:20:45] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:20:50] Joining segment hits [2018-10-13 16:23:20] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:23:25] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:23:29] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:23:34] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:23:38] Joining segment hits [2018-10-13 16:26:20] Reporting output tracks ----------------------------------------------- [2018-10-13 16:29:27] A summary of the alignment counts can be found in /scratch/8793342.1.linga/tophat2/align_summary.txt [2018-10-13 16:29:27] Run complete: 00:28:39 elapsed