[2018-10-13 15:56:20] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:56:20] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:56:20] Checking for Bowtie index files (genome).. [2018-10-13 15:56:20] Checking for reference FASTA file [2018-10-13 15:56:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:56:22] Reading known junctions from GTF file [2018-10-13 15:56:24] Preparing reads left reads: min. length=100, max. length=100, 260659 kept reads (172 discarded) right reads: min. length=100, max. length=100, 260521 kept reads (310 discarded) [2018-10-13 15:56:32] Building transcriptome data files /scratch/8793338.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:56:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 16:01:24] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:01:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 16:01:51] Resuming TopHat pipeline with unmapped reads [2018-10-13 16:01:51] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:02:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:02:07] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:02:11] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:02:15] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:02:19] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 16:02:31] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 16:02:35] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 16:02:40] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 16:02:44] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 16:02:48] Searching for junctions via segment mapping [2018-10-13 16:04:06] Retrieving sequences for splices [2018-10-13 16:05:13] Indexing splices [2018-10-13 16:05:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:05:25] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:05:27] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:05:29] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:05:32] Joining segment hits [2018-10-13 16:06:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:06:42] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:06:45] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:06:47] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:06:49] Joining segment hits [2018-10-13 16:07:58] Reporting output tracks ----------------------------------------------- [2018-10-13 16:09:31] A summary of the alignment counts can be found in /scratch/8793338.1.p16/tophat2/align_summary.txt [2018-10-13 16:09:31] Run complete: 00:13:11 elapsed